Anticorps Polyclonal de lapin anti-ZXDC

ZXDC Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 20530-1-AP

Synonymes

Zinc finger protein ZXDC, ZXD family zinc finger C, ZXD like zinc finger protein, ZXDC, ZXDL

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Galerie de données de validation

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  WB analysis of human kidney using 20530-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 20530-1-AP (ZXDC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20530-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20530-1-AP (ZXDC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 20530-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20530-1-AP (ZXDC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20530-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20530-1-AP (ZXDC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 20530-1-AP

  Immunofluorescent analysis of Hela cells, using ZXDC antibody 20530-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu rénal humain, cellules A431, cellules HEK-293, tissu rénal de souris
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

20530-1-AP cible ZXDC dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZXDC Protéine recombinante Ag14392
• Nom complet: ZXD family zinc finger C
• Masse moléculaire calculée: 858 aa, 90 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC002940
• Symbole du gène: ZXDC
• Identification du gène (NCBI): 79364
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IF	Nat Commun; Cell-fate transition and determination analysis of mouse male germ cells throughout development.; Authors - Jiexiang Zhao
human	WB,IHC	iScience; ZXDC enhances cervical cancer metastasis through IGF2BP3-mediated activation of RhoA/ROCK signaling; Authors - Yifang Mao
human,mouse	WB	Cancer Cell; The crosstalk between macrophages and cancer cells potentiates pancreatic cancer cachexia; Authors - Mingyang Liu

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