Anticorps Monoclonal anti-Alpha Synuclein

Alpha Synuclein Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

1B10E9

Avis (4)

N° de cat : 66412-1-Ig

Synonymes

Alpha synuclein, a-Synuclein, NACP, PARK1, PARK4, PD1, SNCA

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Galerie de données de validation

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  WB analysis of mouse brain using 66412-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66412-1-Ig (a-Synuclein Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66412-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66412-1-Ig (a-Synuclein Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 66412-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 66412-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 66412-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66412-1-Ig (Alpha Synuclein antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 66412-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66412-1-Ig (Alpha Synuclein antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 66412-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66412-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66412-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu de cervelet de souris, tissu cérébral de rat, tissu cérébral de souris, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

66412-1-Ig cible Alpha Synuclein dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Alpha Synuclein Protéine recombinante Ag1285
• Nom complet: synuclein, alpha (non A4 component of amyloid precursor)
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 18-19 kDa
• Numéro d’acquisition GenBank: BC013293
• Symbole du gène: SNCA
• Identification du gène (NCBI): 6622
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Alpha Synuclein (α-syn) is a 14-19 kDa phosphoprotein that is primarily localize to the presynaptic terminals of mature neurons, where it is involved in synaptic function and plasticity. α-syn has drawn intense interest ever since the late 1990s, when the first α-synuclein missense mutation was identified as a cause of familial Parkinson's disease (PD). Aggregated and hyper-phosphorylated forms of α-syn protein are the pathological hallmark of Lewy body disease, which includes Parkinson's disease (PD), diffuse Lewy body disease (DLBD), and Lewy body variant of Alzheimer's disease (LBV). This antibody can recognize all the isoforms of α-syn.

Publications

Species	Application	Title
human	IF	Appl Microbiol Biotechnol; The alteration of intestinal mucosal α-synuclein expression and mucosal microbiota in Parkinson's disease; Authors - Jihua Shi
rat	IHC	Biomed Pharmacother; Nardosinone regulates the slc38a2 gene to alleviate Parkinson's symptoms in rats through the GABAergic synaptic and cAMP pathways.; Authors - Li-Hua Bian
rat	IHC	J Neuropathol Exp Neurol; Regular Aerobic Exercise-Alleviated Dysregulation of CAMKIIα Carbonylation to Mitigate Parkinsonism via Homeostasis of Apoptosis With Autophagy.; Authors - Wenfeng Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Janelle (Verified Customer) (09-12-2022)

Perfect for WB!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

BISHR (Verified Customer) (05-10-2022)

I used it in WB with 1:1000 ration, the band was clear and almost perfect. However, it says the expected kd ranges from 14 to 18, unfortunately I am getting far higher bands up to 50 kd sometimes. Although I am using concentrated acrylamide in the gel, it is making it difficult for reviewers to understand that.

• Applications: Western Blot, Immunofluorescence
• Cell Tissue Type: SH-SY5Y

Alpha Synuclein Antibody Western Blot,Immunofluorescence validation ( dilution) in SH-SY5Y (Cat no:66412-1-Ig)

FH

q (Verified Customer) (01-05-2022)

It is good in WB with a specific band at 17 kd around.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mouse brain lysate

Alpha Synuclein Antibody Western Blot validation (1:3000 dilution) in mouse brain lysate (Cat no:66412-1-Ig)

FH

Sheela (Verified Customer) (07-16-2019)

This product worked as expected

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse brain lysate

Alpha Synuclein Antibody Western Blot, validation (1:1000 dilution) in Mouse brain lysate (Cat no:66412-1-Ig)