Anticorps Monoclonal anti-Acetyl-Tubulin (Lys40)

Acetyl-Tubulin (Lys40) Monoclonal Antibody for IF/ICC, IF-Fro

Hôte / Isotype

Mouse / IgG1

Réactivité testée

canin, Humain, porc, rat, souris

Applications

IF/ICC, IF-Fro

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications(3)

CloneNo.

7E5H8

N° de cat : CL488-66200

Synonymes

acetylated alpha tubulin, TUBA1A, Tubulin alpha 1A chain, 7E5H8, Detyrosinated tubulin alpha-1A chain

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Galerie de données de validation

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  IF Staining of mouse kidney using CL488-66200

  Frozen tissue section of adult mouse kidney was stained for acetylated ɑ-tubulin (magenta, Cat. No CL488-66200), CD-31/PECAM-1 (white), and Shh (green, Cat. No 20697-1-AP) with DAPI as a counterstain for visualizing the nucleus (blue). acetylated ɑ-tubulin stains primary cilia and was conjugated to Coralite-488 fluorescent dye and psedocolored to magenta. CD-31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody and psedocolored to white. Shh stains Sonic Hedgehog and was visualized with an Alexa Fluor 555 secondary antibody and psedocolored to green.The Image and figure legends are intellectual property of @LAF_in_the_LAB.

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  IF Staining of MDCK using CL488-66200

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CL488-66200 (GREEN, Acetyl-Tubulin (Lys40) antibody) at dilution of 1:50 and CL594-17711 (RED, ARL13B antibody) at dilution of 1:50.

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  IF Staining of hTERT-RPE1 using CL488-66200

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 488 Acetyl-Tubulin (Lys40) antibody (CL488-66200, Clone: 7E5H8 ) at dilution of 1:200, CoraLite®594 ARL13B antibody (CL594-17711, red).

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Applications testées

• Résultats positifs en IF-Fro: tissu rénal de souris,
• Résultats positifs en IF/ICC: cellules MDCK, cellules hTERT-RPE1

Dilution recommandée

• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 3 publications below

Informations sur le produit

CL488-66200 cible Acetyl-Tubulin (Lys40) dans les applications de IF/ICC, IF-Fro et montre une réactivité avec des échantillons canin, Humain, porc, rat, souris

• Réactivité: canin, Humain, porc, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tubulin, alpha 1a
• Masse moléculaire calculée: 52 kDa
• Numéro d’acquisition GenBank: NM_006009
• Symbole du gène: Alpha Tubulin
• Identification du gène (NCBI): 7846
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tubulin, composed of heterodimers of alpha and beta tubulin, is the mainly component of microtubules which play important roles in cell motility, mitosis, and intracellular vesicle transport. Both alpha and beta tubulin undergo several posttranslational modifications such as polyglutamylation and acetylation/deacetylation. Tubulin acetylation occurs on lysine-40 at the N-terminal of alpha tubulin and is conserved across species. The histone deacetylase HDAC6 and SIRT2 has been identified as tubulin deacetylases. Reversible acetylation of alpha tubulin may be implicated in regulating microtubule stability, cell motility, and axon regeneration. The level of acetylated tubulin has been linked to epithelial malignancies and sensitivity to chemotherapy. In addition, acetylated tubulin has been widely used as a marker for primary cilia. This antibody is specific to the acetylated tubulin; it does not recognize non-acetylated tubulin. (24268707, 23881549). This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Publications

Species	Application	Title
rat	IF	Front Cell Dev Biol; PIH1D3-knockout rats exhibit full ciliopathy features and dysfunctional pre-assembly and loading of dynein arms in motile cilia; Authors - Tingting Zhang
human	IF	STAR Protoc; Isolation, expansion, differentiation, and histological processing of human nasal epithelial cells; Authors - Vincent Manna
human	IF	BBA Adv; The dynamic nature of the coronavirus receptor, angiotensin-converting enzyme 2 (ACE2) in differentiating airway epithelia.; Authors - Vincent J Manna

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