Anticorps Monoclonal anti-Alpha Tubulin

Alpha Tubulin Monoclonal Antibody for FC (Intra), IF, WB

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

canin, Humain, rat, souris

Applications

WB, IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications

3

CloneNo.

1E4C11

N° de cat : CL488-66031

Synonymes

Alpha Tubulin, Alpha tubulin ubiquitous, alpha tubulin,a tubulin, K ALPHA 1, TUBA1B, Tubulin alpha 1B chain, Tubulin alpha ubiquitous chain, Tubulin K alpha 1, tubulin, alpha 1b, α-tubulin

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Galerie de données de validation

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  WB analysis using CL488-66031

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66031 (Alpha Tubulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse embryo using CL488-66031

  E14.5 FFPE mouse embryo section stained for smooth muscle actin (red, Cat. No 80008-1-RR) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see muscle tissue clearly stained including the tongue, heart, and arteries. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram

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  IF Staining of HeLa using CL488-66031

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using the CoraLite® Plus 488-conjugated version of this antibody, CL488-66031 (alpha Tubulin antibody), at dilution of 1:100.

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  IF Staining of mouse embryo using CL488-66031

  E14.5 FFPE mouse embryo section stained for E-cadherin (red, Cat. No 20874-1-AP) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see the epithelial layer of tissue clearly stained, particularly the skin, eyes, and respiratory system. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram

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  FC experiment of HepG2 using CL488-66031

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Alpha Tubulin (CL488-66031, Clone:1E4C11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules NIH/3T3
• Résultats positifs en IF: cellules HeLa, tissu embryonnaire de souris
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:600
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 2 publications below

Informations sur le produit

CL488-66031 cible Alpha Tubulin dans les applications de WB, IF, FC (Intra) et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Alpha Tubulin Protéine recombinante Ag18034
• Nom complet: tubulin, alpha 1b
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC009314
• Symbole du gène: TUBA1B
• Identification du gène (NCBI): 10376
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. This anitbody is CL488(Ex/Em 488 nm/515 nm) conjugated.

Publications

Species	Application	Title
	IF	Free Radic Biol Med; Iron overload modulates follicular microenvironment via ROS/HIF-1α/FSHR signaling; Authors - Yaoqiu Wu
		Clin Genet; A novel homozygous CFAP65 mutation in humans causes male infertility with multiple morphological abnormalities of the sperm flagella.; Authors - Xueguang Zhang
human	IF	Transl Androl Urol; Novel bi-allelic mutations in DNAH1 cause multiple morphological abnormalities of the sperm flagella resulting in male infertility.; Authors - Chuan Jiang

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C