Anticorps Monoclonal anti-Alpha Tubulin

Alpha Tubulin Monoclonal Antibody for FC, IF, WB

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

canin, Humain, rat, souris

Applications

WB, IF, FC

Conjugaison

CoraLite®594 Fluorescent Dye

Publications

3

CloneNo.

1E4C11

N° de cat : CL594-66031

Synonymes

Alpha Tubulin, Alpha tubulin ubiquitous, alpha tubulin,a tubulin, K ALPHA 1, TUBA1B, Tubulin alpha 1B chain, Tubulin alpha ubiquitous chain, Tubulin K alpha 1, tubulin, alpha 1b, α-tubulin

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Galerie de données de validation

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  WB analysis using CL594-66031

  Various lysates were subjected to SDS PAGE followed by western blot with CL594-66031 (Alpha Tubulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using CL594-66031

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using the CoraLite®594-conjugated version of this antibody, CL594-66031 (alpha Tubulin antibody), at dilution of 1:100.

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  IF Staining of HepG2 using CL594-66031

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66031 (alpha Tubulin antibody) at dilution of 1:100.

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  IF Staining of HepG2 using CL594-66031

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66031 (alpha Tubulin antibody) at dilution of 1:50.

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  FC experiment of HepG2 using CL594-66031

  1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Alpha Tubulin (CL594-66031, Clone:1E4C11) (red), or 0.8 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HSC-T6, cellules NIH/3T3
• Résultats positifs en IF: cellules HepG2, cellules HeLa
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 3 publications below

Informations sur le produit

CL594-66031 cible Alpha Tubulin dans les applications de WB, IF, FC et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Alpha Tubulin Protéine recombinante Ag18034
• Nom complet: tubulin, alpha 1b
• Masse moléculaire calculée: 50 kDa
• Numéro d’acquisition GenBank: BC009314
• Symbole du gène: TUBA1B
• Identification du gène (NCBI): 10376
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. This anitbody is CL594(Ex/Em 593 nm/614 nm) conjugated.

Publications

Species	Application	Title
human	IF	Exp Mol Med; Identification of novel rheumatoid arthritis-associated MiRNA-204-5p from plasma exosomes.; Authors - Long-Fei Wu
mouse	IF	Biology (Basel); Effects of IGF-1 on the Three-Dimensional Culture of Ovarian Preantral Follicles and Superovulation Rates in Mice.; Authors - Shizhen Dai
mouse	IF	Int J Mol Sci; Overexpression of Tfap2a in Mouse Oocytes Impaired Spindle and Chromosome Organization; Authors - Juan Lin