Anticorps Polyclonal de lapin anti-c-Met (Cytoplasmic)

• Phare
• Validé par KD/KO

c-Met (Cytoplasmic) Polyclonal Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

N° de cat : CL488-25869

Synonymes

MET, c-Met, c-Met (Cytoplasmic), EC:2.7.10.1, HGF receptor, HGF/SF receptor, HGFR, AUTS9, c Met, Proto oncogene c Met, Proto-oncogene c-Met, RCCP2, Scatter factor receptor, SF receptor, Tyrosine protein kinase Met, Tyrosine-protein kinase Met

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Galerie de données de validation

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  FC experiment of HeLa using CL488-25869

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human c-Met (Cytoplasmic) (CL488-25869) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-25869 cible c-Met (Cytoplasmic) dans les applications de FC (Intra) et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: c-Met (Cytoplasmic) Protéine recombinante Ag23140
• Nom complet: met proto-oncogene (hepatocyte growth factor receptor)
• Masse moléculaire calculée: 1390 aa, 155 kDa
• Poids moléculaire observé: 145 kDa
• Numéro d’acquisition GenBank: BC130420
• Symbole du gène: MET
• Identification du gène (NCBI): 4233
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

c-Met (also named as MET or HGFR) is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to HGF ligand. c-Met regulates many physiological processes including proliferation, scattering, morphogenesis and survival. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Overexpression and/or mutation of c-Met has been reported in various human malignancies, including lung cancer, breast cancer, head and neck cancer, gastric cancer, colorectal cancer, bladder cancer, uterine cervix carcinoma, and esophageal carcinoma, c-Met could serve as an important therapeutic target (PMID: 26036285). The c-met receptor is a 190-kD glycoprotein consisting of a 145-kD membrane-spanning beta chain and a 50-kD alpha chain (PMID: 7806559). In Western blot, this antibody produces bands of unknown identity at 55 and 100 kDa.

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