Anticorps Polyclonal de lapin anti-calreticulin

• Phare
• Validé par KD/KO

calreticulin Polyclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 555 Fluorescent Dye

Publications(1)

N° de cat : CL555-27298

Synonymes

CALR, Calregulin, CRT

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Galerie de données de validation

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  IF Staining of HepG2 using CL555-27298

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 555 calreticulin antibody (CL555-27298) at dilution of 1:200, CL488-phalloidin (green).

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  IF Staining of SKOV-3 using CL555-27298

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using CoraLite® Plus 555 calreticulin antibody (CL555-27298) at dilution of 1:200.

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  IF Staining of U2OS using CL555-27298

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CoraLite® Plus 555 calreticulin antibody (CL555-27298) at dilution of 1:200.

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  FC experiment of HeLa using CL555-27298

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 555 Anti-Human calreticulin (CL555-27298) (red), or 0.4 ug CoraLite®555-conjugated Rabbit IgG control Rabbit PolyAb (CL555-30000, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Applications testées

• Résultats positifs en IF/ICC: cellules U2OS, cellules HepG2, cellules SKOV-3
• Résultats positifs en FC (Intra): cellules HeLa

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

CL555-27298 cible calreticulin dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: calreticulin Protéine recombinante Ag26064
• Nom complet: calreticulin
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC002500
• Symbole du gène: Calreticulin
• Identification du gène (NCBI): 811
• Conjugaison: CoraLite® Plus 555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 554 nm / 570 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CALR, also named as grp60, ERp60, HACBP, CRP55, CRTC and Calregulin, belongs to the calreticulin family. It is a molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. CALR is an ER marker. It interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. CALR interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. The MW of CALR migrates aberrantly at 60 kD by SDS-PAGE. Some study provided that it's a new possibility for CRT-mediated tumor immune prevention and treatment.

Publications

Species	Application	Title
mouse	IF	Biomaterials; A cuproptosis-based nanomedicine suppresses triple negative breast cancers by regulating tumor microenvironment and eliminating cancer stem cells; Authors - Chen Xiao

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