Anticorps Monoclonal anti-citrate synthase

citrate synthase Monoclonal Antibody for WB, IHC, IF/ICC, IF-Fro, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-Fro, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

2F9G6

Avis (1)

N° de cat : 67784-1-Ig

Synonymes

CS, 2F9G6, CISY, Citrate, Citrate synthase, mitochondrial

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Galerie de données de validation

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  WB analysis using 67784-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67784-1-Ig (citrate synthase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 67784-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67784-1-Ig (CS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67784-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67784-1-Ig (citrate synthase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67784-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67784-1-Ig (citrate synthase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse breast cancer using 67784-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using citrate synthase antibody (67784-1-Ig, Clone: 2F9G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of HepG2 using 67784-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using citrate synthase antibody (67784-1-Ig, Clone: 2F9G6 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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  IF Staining of MCF-7 using 67784-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using citrate synthase antibody (67784-1-Ig, Clone: 2F9G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules PC-12
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-Fro: mouse breast cancer,
• Résultats positifs en IF/ICC: cellules MCF-7, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

67784-1-Ig cible citrate synthase dans les applications de WB, IHC, IF/ICC, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: citrate synthase Protéine recombinante Ag9255
• Nom complet: citrate synthase
• Masse moléculaire calculée: 466 aa, 52 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC010106
• Symbole du gène: Citrate Synthase
• Identification du gène (NCBI): 1431
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Citrate synthase (CS), the first and rate-limiting enzyme of the tricarboxylic acid cycle, plays a key role in regulating energy generation of mitochondrial respiration(PMID:19479947).It belongs to the citrate synthase family. The deduced 466-amino acid protein contains an N-terminal mitochondrial targeting sequence and a motif highly conserved in citrate synthases(PMID:12549038). It can exsit as a dimer(PMID:8749851). Northern blot analysis detected no CS expression in thymus and small intestine(PMID:12549038). This antibody is specific to CS.

Publications

Species	Application	Title
human	WB	Cell Death Dis; BNIP3-mediated mitophagy boosts the competitive growth of Lenvatinib-resistant cells via energy metabolism reprogramming in HCC; Authors - Sikai Wang
mouse	WB	Aging Cell; Ketogenic β-hydroxybutyrate regulates β-hydroxybutyrylation of TCA cycle-associated enzymes and attenuates disease-associated pathologies in Alzheimer's mice; Authors - Wanhong Han

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mi (Verified Customer) (02-21-2023)

It works very well in adipocytes.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: adipocytes

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