Anticorps Polyclonal de lapin anti-GST Tag
GST Tag Polyclonal Antibody for FC, WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Protéine recombinante et plus (2)
Applications
WB, IP, IF, CoIP, ChIP, ELISA
Conjugaison
Non conjugué
188
N° de cat : 10000-0-AP
Synonymes
"GST Tag Antibodies" Comparison
View side-by-side comparison of GST Tag antibodies from other vendors to find the one that best suits your research needs.
Applications testées
| Résultats positifs en WB | Protéine recombinante |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 125 publications below |
| IF | See 2 publications below |
| IP | See 18 publications below |
| ELISA | See 1 publications below |
| CoIP | See 4 publications below |
| ChIP | See 2 publications below |
Informations sur le produit
10000-0-AP cible GST Tag dans les applications de WB, IP, IF, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Protéine recombinante
| Réactivité | Protéine recombinante |
| Réactivité citée | Humain, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | Protéine recombinante |
| Nom complet | GST Tag |
| Masse moléculaire calculée | 26 kDa |
| Numéro d’acquisition GenBank | FN315687 |
| Symbole du gène | |
| Identification du gène (NCBI) | |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein. This antibody can be used as Rabbit IgG control in FC.
Publications
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis | ||
Gastroenterology PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis | ||
Ann Rheum Dis Autoantibodies identify primary Sjögren's syndrome in patients lacking serum IgG specific for Ro/SS-A and La/SS-B | ||
Immunity Targeting 7-Dehydrocholesterol Reductase Integrates Cholesterol Metabolism and IRF3 Activation to Eliminate Infection. | ||
Nat Struct Mol Biol CRISPR-Cas9-based functional interrogation of unconventional translatome reveals human cancer dependency on cryptic non-canonical open reading frames | ||
Nat Commun PHGDH arginine methylation by PRMT1 promotes serine synthesis and represents a therapeutic vulnerability in hepatocellular carcinoma |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Daniel (Verified Customer) (06-10-2022) | We use this anti-GST antibody for GST fusion protein-lipid interactions. We found this antibody gives much less background than other antibodies.
 |
FH Lana (Verified Customer) (04-06-2022) | SDS-PAGE: 4-12% Bis-Tris gradient gel. Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C. Blocking: SEA Block Blocking Buffer 1h, room T. Primary Ab: O/N incubation at 4C, 1:1000. Secondary Ab: IRDye 680LT Goat anti-Rabbit, 1:15000. Lines of WB image: 1 – protein ladder, 2 – mitochondria fraction lysate, 3 – GST protein, 4 – GST fusion protein
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FH Shihab (Verified Customer) (10-28-2021) | Tried this antibody for a control experiment and worked nicely. Will be continuing to try this in immunofluorescence
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FH Amie (Verified Customer) (07-27-2021) | I compared this antibody directly to the proteintech mouse monoclonal (66001-2-Ig). In the provided image, the first 4 lanes of each gel are identical. The rabbit antibody seems to be slightly better (less background) than the mouse.
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FH Yan (Verified Customer) (02-20-2021) | It worked very well when I performed western blot after immunoprecipitation.
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