Anticorps Polyclonal de lapin anti-ki67

ki67 Polyclonal Antibody for FC, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris et plus (2)

Applications

WB, IHC, IF, FC, Cell treatment, ELISA

Conjugaison

Non conjugué

Publications

23

Avis (1)

N° de cat : 28074-1-AP

Synonymes

D630048A14Rik, Ki 67, Ki67, Mki67

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Galerie de données de validation

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  IHC staining of mouse spleen using 28074-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28074-1-AP (ki67 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 28074-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28074-1-AP (ki67 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 28074-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using ki67 antibody (28074-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of mouse spleen using 28074-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using ki67 antibody (28074-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse spleen using 28074-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using ki67 antibody (28074-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse colon using 28074-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using ki67 antibody (28074-1-AP) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse colon using 28074-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using ki67 antibody (28074-1-AP) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of NIH/3T3 using 28074-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Mouse ki67 (28074-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IHC: tissu splénique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules NIH/3T3, tissu de côlon de souris, tissu splénique de souris
• Résultats positifs en cytométrie: cellules NIH/3T3

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:400-1:1600
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 11 publications below
• IF: See 7 publications below

Informations sur le produit

28074-1-AP cible ki67 dans les applications de WB, IHC, IF, FC, Cell treatment, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ki67 Protéine recombinante Ag27894
• Nom complet: antigen identified by monoclonal antibody Ki 67
• Masse moléculaire calculée: 351 kDa
• Numéro d’acquisition GenBank: NM_001081117
• Symbole du gène: Mki67
• Identification du gène (NCBI): 17345
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IF	Biosens Bioelectron; 3D co-culture of macrophages and fibroblasts in a sessile drop array for unveiling the role of macrophages in skin wound-healing; Authors - Xiaoyan Lyu
mouse	IHC	Phytomedicine; Kaempferol inhibits non-homologous end joining repair via regulating Ku80 stability in glioma cancer; Authors - Meiyang Chen
mouse	IHC	Biochem Pharmacol; Targeting HMGA1 contributes to immunotherapy in aggressive breast cancer while suppressing EMT; Authors - Xing Chang
human	WB,IF	Genes Cells; Local anesthetic lidocaine induces growth suppression of HeLa cells by decreasing and changing the cellular localization of the proliferation marker Ki-67; Authors - Keiko Haraguchi-Suzuki
mouse	IHC	J Oncol; EGCG Enhances the Chemosensitivity of Colorectal Cancer to Irinotecan through GRP78-MediatedEndoplasmic Reticulum Stress; Authors - Wenbing Wu
human	IHC	In Vitro Cell Dev Biol Anim; TRIM47 promotes glioma angiogenesis by suppressing Smad4; Authors - Zifan Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ruchi (Verified Customer) (06-08-2023)

Stained Ki67 on murine diabetic skin

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Mouse skin

ki67 Antibody Immunofluorescence validation (1:50 dilution) in Mouse skin (Cat no:28074-1-AP)

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011