Anticorps Polyclonal de lapin anti-Ki-67

Ki-67 Polyclonal Antibody for IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA, Cell treatment

Conjugaison

Non conjugué

Publications(133)

Avis (1)

N° de cat : 28074-1-AP

Synonymes

Ki 67, ki67, Mki67, Proliferation related Ki 67 antigen

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Galerie de données de validation

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  IHC staining of mouse spleen using 28074-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28074-1-AP (ki67 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 28074-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28074-1-AP (ki67 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using 28074-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using ki67 antibody (28074-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse spleen using 28074-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using ki67 antibody (28074-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse colon using 28074-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using ki67 antibody (28074-1-AP) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse colon using 28074-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using ki67 antibody (28074-1-AP) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse spleen using 28074-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse spleen tissue using ki67 antibody (28074-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of mouse spleen using 28074-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse spleen tissue using ki67 antibody (28074-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of mouse liver using 28074-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse liver tissue using Ki-67 antibody (28074-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

• 

  IF Staining of NIH/3T3 using 28074-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using ki67 antibody (28074-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 28074-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ki67 antibody (28074-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

• 

  FC experiment of NIH/3T3 using 28074-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Mouse ki67 (28074-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IHC: tissu splénique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de côlon de souris, tissu splénique de souris
• Résultats positifs en IF-Fro: tissu splénique de souris, tissu hépatique de souris
• Résultats positifs en IF/ICC: cellules NIH/3T3, cellules HeLa
• Résultats positifs en FC (Intra): cellules NIH/3T3

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:1500-1:6000
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 22 publications below
• IHC: See 93 publications below
• IF: See 26 publications below

Informations sur le produit

28074-1-AP cible Ki-67 dans les applications de WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA, Cell treatment et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Ki-67 Protéine recombinante Ag27894
• Nom complet: antigen identified by monoclonal antibody Ki 67
• Masse moléculaire calculée: 351 kDa
• Numéro d’acquisition GenBank: NM_001081117
• Symbole du gène: ki67
• Identification du gène (NCBI): 17345
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry.

Publications

Species	Application	Title
mouse	IHC	Drug Resist Updat; Lansoprazole (LPZ) reverses multidrug resistance (MDR) in cancer through impeding ATP-binding cassette (ABC) transporter-mediated chemotherapeutic drug efflux and lysosomal sequestration; Authors - Ning Ji
		Cell Mol Immunol; Targeting PHGDH reverses the immunosuppressive phenotype of tumor-associated macrophages through α-ketoglutarate and mTORC1 signaling; Authors - Zhengnan Cai
mouse	IF	Biosens Bioelectron; 3D co-culture of macrophages and fibroblasts in a sessile drop array for unveiling the role of macrophages in skin wound-healing; Authors - Xiaoyan Lyu
mouse	IHC	Adv Healthc Mater; A Multifunctional Nanodrug Increases the Therapeutic Sensitivity of Lenvatinib to Hepatocellular Carcinoma by Inhibiting the Stemness of Hepatic Cancer Stem Cells; Authors - Xieqing Yang
mouse	IHC	Cancer Lett; Icaritin with autophagy/mitophagy inhibitors synergistically enhances anticancer efficacy and apoptotic effects through PINK1/Parkin-mediated mitophagy in hepatocellular carcinoma; Authors - Piao Luo
mouse	IHC	ACS Appl Mater Interfaces; Doxorubicin-Loaded Microalgal Delivery System for Combined Chemotherapy and Enhanced Photodynamic Therapy of Osteosarcoma; Authors - Xueying An

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ruchi (Verified Customer) (06-08-2023)

Stained Ki67 on murine diabetic skin

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Mouse skin

Ki-67 Antibody Immunofluorescence validation (1:50 dilution) in Mouse skin (Cat no:28074-1-AP)

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