Anticorps Polyclonal de lapin anti-Lamin A/C

• Phare
• Validé par KD/KO

Lamin A/C Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

183

Avis (5)

N° de cat : 10298-1-AP

Synonymes

70 kDa lamin, CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, HGPS, IDC, lamin A, lamin A/C, LDP1, LFP, LGMD1B, LMN1, LMNA, LMNC, Prelamin A/C, PRO1, progerin

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• Galerie de données de validation
• Proteintech vs. Other Vendors

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  WB analysis using 10298-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10298-1-AP (Lamin A/C antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10298-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10298-1-AP (Lamin A/C antibody) at dilution of 1:35000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10298-1-AP

  WB result of Lamin A/C antibody (10298-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin A/C transfected HeLa cells.

• 

  WB analysis of C6 using 10298-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10298-1-AP (Lamin A/C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10298-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10298-1-AP (lamin-A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 10298-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10298-1-AP (lamin-A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 10298-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10298-1-AP (lamin-A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 10298-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10298-1-AP (lamin-A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10298-1-AP

  IP result of anti-Lamin A/C(IP:10298-1-AP, 4ug; Detection:10298-1-AP 1:50000) with HeLa cells lysate 1360 ug.

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  IP experiment of A375 using 10298-1-AP

  IP Result of anti-lamin-A (IP:10298-1-AP, 3ug; Detection:10298-1-AP 1:1000) with A375 cells lysate 800ug.

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  IHC staining of mouse heart using 10298-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10298-1-AP (Lamin A/C antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 10298-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10298-1-AP (Lamin A/C antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10298-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10298-1-AP (Lamin A/C antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10298-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Lamin A/C antibody (10298-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 10298-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10298-1-AP (Lamin A/C antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10298-1-AP

  Immunofluorescent analysis of (-20°C Acetone) fixed HeLa cells using 10298-1-AP (Lamin A/C antibody) at dilution of 1:100 and CL594-66467 (CL594-Mouse anti-Rabbit IgG heavy chain) as secondary antibody with dilution 1:400. .

• 

  FC experiment of HEK-293T using 10298-1-AP

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human Lamin A/C (10298-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A375, cellules C6, cellules HEK-293, cellules HeLa, cellules HUVEC, cellules NIH/3T3, cellules SKOV-3, tissu ovarien de souris
• Résultats positifs en IP: cellules HeLa, cellules A375
• Résultats positifs en IHC: tissu cardiaque de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules HeLa
• Résultats positifs en cytométrie: cellules HEK-293T

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF): IF : 1:400-1:1600
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 172 publications below
• IHC: See 1 publications below
• IF: See 8 publications below
• IP: See 3 publications below

Informations sur le produit

10298-1-AP cible Lamin A/C dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, singe, souris, duck
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Lamin A/C Protéine recombinante Ag0408
• Nom complet: lamin A/C
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 65 kDa, 70 kDa
• Numéro d’acquisition GenBank: BC003162
• Symbole du gène: LMNA
• Identification du gène (NCBI): 4000
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Lamin A/C is also named as LMNA or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure, and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb-girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa, and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.

Publications

Species	Application	Title
human	IF	Nat Microbiol; Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus.; Authors - Adarsh Dharan
human	WB	Mol Cell; Lactylation-driven METTL3-mediated RNA m6A modification promotes immunosuppression of tumor-infiltrating myeloid cells.; Authors - Jia Xiong
human	WB	J Extracell Vesicles; Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.; Authors - Xiaohan Gao
human	WB	Cancer Cell; SET1A-Mediated Mono-Methylation at K342 Regulates YAP Activation by Blocking Its Nuclear Export and Promotes Tumorigenesis.; Authors - Lan Fang
human	WB	Sci Adv; XAF1 promotes anti-RNA virus immune responses by regulating chromatin accessibility; Authors - Ming Kuang
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alejandro (Verified Customer) (08-22-2022)

Shows clear staining in IF and well staining using FACS

• Applications: Immunofluorescence, Flow Cytometry
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Bone marrow derived macrophages

Lamin A/C Antibody Immunofluorescence,Flow Cytometry validation (1/100 dilution) in Bone marrow derived macrophages (Cat no:10298-1-AP)

FH

Charlotte (Verified Customer) (07-29-2022)

Cell fraction performed on NIH-3T3 cells to show the nucleus part. Blot super clean. Antibody specific. Easy to reveal.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: NIH-3T3

Lamin A/C Antibody Western Blot validation (1/1000 dilution) in NIH-3T3 (Cat no:10298-1-AP)

FH

S (Verified Customer) (12-31-2021)

good antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

Lamin A/C Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:10298-1-AP)

FH

Azita (Verified Customer) (06-16-2021)

It was used as a marker in WB to confirm proper nuclear fractionation of cell lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: NSC-34 cell line

FH

Declan (Verified Customer) (11-29-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1/5000
• Cell Tissue Type: Human brain homogenate

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