Anticorps Polyclonal de lapin anti-mCherry

mCherry Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Protéine recombinante et plus (7)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(111)

Avis (8)

N° de cat : 26765-1-AP

Synonymes

RFP-tag, RFP, red fluorescent protein, Red fluorescence protein

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Galerie de données de validation

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  WB analysis using 26765-1-AP

  mCherry tagged protein was subjected to SDS PAGE followed by western blot with 26765-1-AP (mCherry antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 26765-1-AP

  WB result of mCherry antibody (26765-1-AP; 1:2000; room temperature for 1.5 hours) with negative control and mCherry overexpressed Transfected HEK-293 cells.

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  WB analysis of Transfected HEK-293 using 26765-1-AP

  WB result of mCherry antibody (26765-1-AP; 1:300; room temperature for 1.5 hours) with negative control and mCherry overexpressed Transfected HEK-293 cells.

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  IP experiment of Transfected HEK-293T using 26765-1-AP

  IP result of anti-mCherry (IP:26765-1-AP, 4ug; Detection:26765-1-AP 1:2000) with Transfected HEK-293T cells lysate 1280 ug.

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  IF Staining of Transfected HEK-293 using 26765-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using mCherry antibody (26765-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293 transfectées, Protéine recombinante
• Résultats positifs en IP: Transfected HEK-293T cells,
• Résultats positifs en IF/ICC: cellules HEK-293 transfectées,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 67 publications below
• IHC: See 4 publications below
• IF: See 39 publications below
• IP: See 10 publications below
• CoIP: See 4 publications below

Informations sur le produit

26765-1-AP cible mCherry dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Réactivité citée: Humain, Lapin, levure, plante, poisson-zèbre, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: mCherry Protéine recombinante Ag25320
• Nom complet: mCherry
• Masse moléculaire calculée: 27 kDa
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Red fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages.

The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm).

When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a rabbit polyclonal antibody raised against mCherry. It can be used to detect mCherry, dsRed, tdTomato, and mScarlet.

Publications

Species	Application	Title
	WB,IP,IF	Cell; The WDR11 complex is a receptor for acidic-cluster-containing cargo proteins; Authors - Huaqing Deng
	WB	Nat Struct Mol Biol; Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis; Authors - Wei Zhang
	WB	Nat Commun; TBC1D23 mediates Golgi-specific LKB1 signaling; Authors - Yingfeng Tu
human	WB	Nat Commun; Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis; Authors - Ji Geng
human	IF	J Extracell Vesicles; Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion; Authors - Chuqi Liu
	WB,IP	Cell Death Differ; RING1 dictates GSDMD-mediated inflammatory response and host susceptibility to pathogen infection; Authors - Yuanyuan Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christine (Verified Customer) (09-23-2025)

Very strong and clean western blot on cells transfected with a mCHERRY tagged protein. Very sensitive antibody, as good as the home made one we were trying to replace. The monoclonal rabbit one sold by Proteintech (81202-2-RR) is also excellent and clean (but a slightly less strong), but would another great choice.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5,000

FH

Alexandra (Verified Customer) (07-02-2024)

Not the strongest antibody I've ever used but it works fine, the maximum recomended dilution is 1000x, but possibly 500x would be more effective.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation, Cell culture
• Primary Antibody Dilution: 1000x
• Cell Tissue Type: U2OS human osteosarcoma cell line

mCherry Antibody Western Blot,Immunofluorescence,Immunoprecipitation,Cell culture validation (1000x dilution) in U2OS human osteosarcoma cell line (Cat no:26765-1-AP)

FH

Verdiana (Verified Customer) (10-12-2023)

Nice and clear bands!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS

FH

Andrea (Verified Customer) (10-05-2023)

Good and strong signal for the mCherry fusion protein. In my case it was a 1:1000 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HCT-116

mCherry Antibody Western Blot validation (1:1000 dilution) in HCT-116 (Cat no:26765-1-AP)

FH

Tatyana (Verified Customer) (01-09-2023)

Excellent antibody, very good bright signal, no background or non-specific bands. HeLa cells were transfected with plasmids for expression of mCherry tagged proteins, lysed after 24 hours, followed by WB (semi-dry transfer, blocking in 4% milk). Antibody was diluted in 4% milk.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Human / HeLa cells

mCherry Antibody Western Blot validation (1:2000 dilution) in Human / HeLa cells (Cat no:26765-1-AP)

FH

Laura (Verified Customer) (01-14-2020)

Antibody was used in 5% TBST overnight, gave a clean blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: HEK293T

FH

Benjamin (Verified Customer) (12-10-2019)

This antibody has been used to image mCherry fusion proteins expressed in HEK293T cells via western blot with high rates of success.Antibody was diluted in 3% milk TBST and incubated overnight at 4 degrees.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: HEK293T Lysate

FH

Liang (Verified Customer) (05-27-2019)

The antibody works perfect. According the attached image, The 2nd from the left is the negative control protein (mGFP) and the 3rd lane is the mCherry protein as the positive control, the others are the target protein.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: purified protein expressed from Ecoli

mCherry Antibody Western Blot,Immunoprecipitation, validation (1:3000 dilution) in purified protein expressed from Ecoli (Cat no:26765-1-AP)

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