Anticorps Polyclonal de lapin anti-mCherry

mCherry Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Protéine recombinante et plus (5)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

57

Avis (6)

N° de cat : 26765-1-AP

Synonymes

mCherry

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Galerie de données de validation

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  WB analysis of Transfected HEK-293 using 26765-1-AP

  WB result of mCherry antibody (26765-1-AP; 1:2000; room temperature for 1.5 hours) with negative control and mCherry overexpressed Transfected HEK-293 cells.

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  WB analysis of Transfected HEK-293 using 26765-1-AP

  WB result of mCherry antibody (26765-1-AP; 1:300; room temperature for 1.5 hours) with negative control and mCherry overexpressed Transfected HEK-293 cells.

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  IF Staining of Transfected HEK-293 using 26765-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using mCherry antibody (26765-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293 transfectées,
• Résultats positifs en IF: cellules HEK-293 transfectées,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 37 publications below
• IHC: See 2 publications below
• IF: See 21 publications below
• IP: See 6 publications below
• CoIP: See 3 publications below

Informations sur le produit

26765-1-AP cible mCherry dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Réactivité citée: Humain, levure, plante, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: mCherry Protéine recombinante Ag25320
• Nom complet: mCherry
• Masse moléculaire calculée: 27 kDa
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

mCherry is a fluorophore (a fluorescent protein) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry and the majority of red fluorescent proteins derive from a protein isolated from Discosoma sp. mCherry is a monomeric fluorescent construct with peak absorption/emission at 587 nm and 610 nm, respectively.

Publications

Species	Application	Title
human	IF	J Extracell Vesicles; Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion; Authors - Chuqi Liu
human	IP	Nat Commun; DELE1 tracks perturbed protein import and processing in human mitochondria.; Authors - Evelyn Fessler
human	IP	Protein Cell; cGAS guards against chromosome end-to-end fusions during mitosis and facilitates replicative senescence.; Authors - Xiaocui Li
human	IF,IP	Dev Cell; ORF3a of SARS-CoV-2 promotes lysosomal exocytosis-mediated viral egress; Authors - Di Chen
	IF	Dev Cell; Centrosomal Localization of RXRα Promotes PLK1 Activation and Mitotic Progression and Constitutes a Tumor Vulnerability.; Authors - Guobin Xie
	WB,IF	Autophagy; Proteotoxic stress disrupts epithelial integrity by inducing MTOR sequestration and autophagy overactivation.; Authors - Xiaoxiang Cheng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Verdiana (Verified Customer) (10-12-2023)

Nice and clear bands!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS

FH

Andrea (Verified Customer) (10-05-2023)

Good and strong signal for the mCherry fusion protein. In my case it was a 1:1000 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HCT-116

mCherry Antibody Western Blot validation (1:1000 dilution) in HCT-116 (Cat no:26765-1-AP)

FH

Tatyana (Verified Customer) (01-09-2023)

Excellent antibody, very good bright signal, no background or non-specific bands. HeLa cells were transfected with plasmids for expression of mCherry tagged proteins, lysed after 24 hours, followed by WB (semi-dry transfer, blocking in 4% milk). Antibody was diluted in 4% milk.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Human / HeLa cells

mCherry Antibody Western Blot validation (1:2000 dilution) in Human / HeLa cells (Cat no:26765-1-AP)

FH

Laura (Verified Customer) (01-14-2020)

Antibody was used in 5% TBST overnight, gave a clean blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: HEK293T

FH

Benjamin (Verified Customer) (12-10-2019)

This antibody has been used to image mCherry fusion proteins expressed in HEK293T cells via western blot with high rates of success.Antibody was diluted in 3% milk TBST and incubated overnight at 4 degrees.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: HEK293T Lysate

FH

Liang (Verified Customer) (05-27-2019)

The antibody works perfect. According the attached image, The 2nd from the left is the negative control protein (mGFP) and the 3rd lane is the mCherry protein as the positive control, the others are the target protein.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: purified protein expressed from Ecoli

mCherry Antibody Western Blot,Immunoprecipitation, validation (1:3000 dilution) in purified protein expressed from Ecoli (Cat no:26765-1-AP)