Anticorps Polyclonal de lapin anti-Nestin

Nestin Polyclonal Antibody for WB, IF/ICC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 29285-1-AP

Synonymes

NES

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Galerie de données de validation

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  WB analysis using 29285-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29285-1-AP (nestin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 29285-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 29285-1-AP (nestin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of rat brain using 29285-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using nestin antibody (29285-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), NeuN antibody (66836-1-Ig, Clone: 3A4C1, red).

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  IF Staining of human kidney using 29285-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Nestin antibody (29285-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of rat brain using 29285-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using nestin antibody (29285-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), GFAP antibody (60190-1-Ig, Clone: 4B2E10, red).

• 

  IF Staining of U-251 using 29285-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using Nestin antibody (29285-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of SH-SY5Y using 29285-1-AP

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human Nestin (29285-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules SGC-7901, cellules MG U-87, cellules U-251
• Résultats positifs en IF-P: tissu cérébral de rat, tissu rénal humain
• Résultats positifs en IF/ICC: cellules U-251,
• Résultats positifs en FC (Intra): cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 2 publications below

Informations sur le produit

29285-1-AP cible Nestin dans les applications de WB, IF/ICC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Nestin Protéine recombinante Ag29865
• Nom complet: nestin
• Masse moléculaire calculée: 177 kDa
• Poids moléculaire observé: 240-280 kDa
• Numéro d’acquisition GenBank: NM_006617
• Symbole du gène: Nestin
• Identification du gène (NCBI): 10763
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Nestin can be detected as 220-240 kDa and 240-280 kDa. Studies point toward nestin heavy posttranslational modifications as possibly serving particular functions in podocytes and in a few other particular cell types(PMID: 17210924).

Publications

Species	Application	Title
human	IF	Bioact Mater; Construction of functional neural network tissue combining CBD-NT3-modified linear-ordered collagen scaffold and TrkC-modified iPSC-derived neural stem cells for spinal cord injury repair; Authors - Zhaoping Wu
mouse,rat	IF	Brain Behav Immun; Microbial reconstitution reverses prenatal stress-induced cognitive impairment and synaptic deficits in rat offspring; Authors - Jie Chen

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