Anticorps Polyclonal de lapin anti-NF-κB p65

• Phare
• Validé par KD/KO

NF-κB p65 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (7)

Applications

WB, IP, IHC, IF, FC, CoIP, ChIP, Cell treatment, ELISA

Conjugaison

Non conjugué

Publications

1151

Avis (7)

N° de cat : 10745-1-AP

Synonymes

NF Kappa B, NF κB, NFKB 3, NFKB p65, NFKB3, NF-κB, NF-κB3, p65, RELA, Transcription factor p65

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Galerie de données de validation

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  WB analysis of Hek-293 cells using 10745-1-AP

  WB result of RELA, p65antibody (10745-1-AP, 1:500) with si-Control and si-RELA,p65 transfected HeLa cells.

• 

  WB analysis using 10745-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10745-1-AP (NF-κB p65 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10745-1-AP

  HeLa nuclear fraction and cytoplasmic fraction were subjected to SDS PAGE saparately, followed by western blot with 10745-1-AP (NF-κB p65 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped, reblotted with lamin B1 (12987-1-AP) & GAPDH (60004-1-Ig) antibodies as the loading control.

• 

  WB analysis of MCF-7 using 10745-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10745-1-AP (p65 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10745-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10745-1-AP (p65 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10745-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10745-1-AP (p65 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10745-1-AP

  IP Result of anti-p65 (IP:10745-1-AP, 3ug; Detection:10745-1-AP 1:1000) with HeLa cells lysate 5000ug.

• 

  IHC staining of human breast cancer using 10745-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10745-1-AP (NF-κB p65 antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10745-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10745-1-AP (p65 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach using 10745-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 10745-1-AP (p65 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 10745-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 10745-1-AP (p65 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 10745-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10745-1-AP (p65 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 10745-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10745-1-AP (p65 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 10745-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10745-1-AP (p65; RELA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 10745-1-AP

  1X10^6 HeLa cells were stained with 0.2ug p65; RELA antibody (10745-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562, cellules MCF-7, cellules NIH/3T3, cellules Raji
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du foie humain, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 20 publications below
• WB: See 1040 publications below
• IHC: See 90 publications below
• IF: See 181 publications below
• IP: See 10 publications below
• ELISA: See 1 publications below
• CoIP: See 6 publications below
• ChIP: See 5 publications below

Informations sur le produit

10745-1-AP cible NF-κB p65 dans les applications de WB, IP, IHC, IF, FC, CoIP, ChIP, Cell treatment, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, Lapin, porc, poulet, singe, souris, Hamster, fish
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NF-κB p65 Protéine recombinante Ag1199
• Nom complet: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC011603
• Symbole du gène: RELA
• Identification du gène (NCBI): 5970
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.

Publications

Species	Application	Title
mouse	IF	Blood; PMID: 32291445; Authors - Kunpeng Wu
mouse	WB	Mol Cell; Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation; Authors - Chuanlong Wang
human	WB	Nat Microbiol; IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection.; Authors - Zhimin Jiang
human	WB	Sci Adv; JLP-centrosome is essential for the microtubule-mediated nucleocytoplasmic transport induced by extracellular stimuli.; Authors - Clement M Lee
human	WB,IF	Nucleic Acids Res; p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway; Authors - Xinxing Zhu
mouse	WB	Nat Commun; REV-ERBα integrates colon clock with experimental colitis through regulation of NF-κB/NLRP3 axis.; Authors - Shuai Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Maximilian (Verified Customer) (06-30-2023)

Quite a bit of unspecific binding. Did not work in mouse cell lines.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mainly Human Breast Cancer Cell lines (MDA-MB-436, MDA-MB-468, MDA-MB-231)

FH

Xie (Verified Customer) (09-29-2022)

NF-κB p65 Antibody staining.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: hela

NF-κB p65 Antibody Immunofluorescence validation (1:250 dilution) in hela (Cat no:10745-1-AP)

FH

Murali (Verified Customer) (01-24-2022)

Very nice antibody for IHC

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1 to 200
• Cell Tissue Type: pharyngeal mucosal

NF-κB p65 Antibody Immunohistochemistry validation (1 to 200 dilution) in pharyngeal mucosal (Cat no:10745-1-AP)

FH

Azita (Verified Customer) (05-31-2021)

NSC-34 cells (motor neuron-like cells) strong labelling in WB

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: Human spinal cord (cervical) tissue

FH

Zehua (Verified Customer) (12-17-2019)

After siRNA treatment for 48 hours, specific signal shown here. Works excellent!

• Applications: Western Blot, Cell culture,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: K562

NF-κB p65 Antibody Western Blot,Cell culture, validation (1:1000 dilution) in K562 (Cat no:10745-1-AP)

FH

Feng-qian (Verified Customer) (12-07-2018)

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: HaCaT

FH

Fraser (Verified Customer) (06-06-2018)

• Applications: Other (please state below),

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