Anticorps Polyclonal de lapin anti-p70(S6K)

• Phare
• Validé par KD/KO

p70(S6K) Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

144

N° de cat : 14485-1-AP

Synonymes

p70 alpha, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K 1, p70 S6K alpha, p70 S6KA, p70(S6K), p70(S6K) alpha, p70S6K, P70S6K1, PS6K, Ribosomal protein S6 kinase I, RPS6KB1, S6K, S6K beta 1, S6K1, STK14A

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Galerie de données de validation

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  WB analysis of MCF-7 using 14485-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14485-1-AP (p70(S6K) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14485-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14485-1-AP (p70(S6K) antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14485-1-AP

  WB result of p70(S6K) antibody (14485-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p70(S6K) transfected MCF-7 cells.

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  WB analysis using 14485-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14485-1-AP (p70(S6K) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14485-1-AP

  IP Result of anti-p70S6K (IP:14485-1-AP, 3ug; Detection:14485-1-AP 1:1000) with HeLa cells lysate 3000ug.

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  IHC staining of human liver cancer using 14485-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14485-1-AP (p70(S6K) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14485-1-AP

  Immunofluorescent analysis of HepG2 cells using 14485-1-AP (p70S6K antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of HepG2 using 14485-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using p70(S6K) antibody (14485-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 14485-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug p70S6K antibody (14485-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules C6, cellules HeLa, cellules Jurkat, cellules MCF-7 traitées à l'IGF-1, cellules NIH/3T3, cellules PC-12, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 140 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

14485-1-AP cible p70(S6K) dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, Lapin, porc, souris, Megalobrama Amblycephala
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: p70(S6K) Protéine recombinante Ag5883
• Nom complet: ribosomal protein S6 kinase, 70kDa, polypeptide 1
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 65-75 kDa, 80-85 kDa
• Numéro d’acquisition GenBank: BC053365
• Symbole du gène: RPS6KB1
• Identification du gène (NCBI): 6198
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RPS6KB1(Ribosomal protein S6 kinase beta-1) is also named as STK14A, p70 S6KA, and belongs to the S6 kinase subfamily. RPS6KB1 is a major substrate of mTOR and acts as a crucial effector of mTOR signaling pathway. It plays a key role in cell growth and proliferation by regulating INS sensitivity, metabolism, protein synthesis, and cell cycle. RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy (PMID:22684641). The Rps6kb1 gene encodes the 70 kDa ribosomal protein S6 kinase (p70S6K). The PI3K/mTOR signalling pathway is one of the major mechanisms for controlling cell survival, proliferation, and metabolism and is the central regulator of translation of some components of the protein synthesis system. Due to alternative translation, two isoform S6K1 proteins are known to exist in mammalian cells: p85 S6K1 and p70 S6K1, which is identical to p85 S6K but lacks its first 23 amino acids. In addition, mammalian cells express a second S6K1 isoform spanning 316 amino acids (p31 S6K1).

Publications

Species	Application	Title
human,mouse	WB	Nature; Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.; Authors - Xiao-Yi Lu
mouse	WB	Cell Stem Cell; Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2α axis.; Authors - Changzheng Li
mouse	IF	Small Methods; PROTAC Degraders of Androgen Receptor-Integrated Dissolving Microneedles for Androgenetic Alopecia and Recrudescence Treatment via Single Topical Administration; Authors - Ruxuan Wang
human	WB	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang
human,mouse	WB	Redox Biol; Aberrant hyper-expression of the RNA binding protein GIGYF2 in endothelial cells modulates vascular aging and function; Authors - Fanglin Niu
mouse	WB	Autophagy; SMCR8 negatively regulates AKT and MTORC1 signaling to modulate lysosome biogenesis and tissue homeostasis.; Authors - Yungang Lan

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