Anticorps Polyclonal de lapin anti-pan-keratin

pan-keratin Polyclonal Antibody for IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF

Conjugaison

CoraLite®555 Fluorescent Dye

N° de cat : CL555-26411

Synonymes

58 kDa cytokeratin, CK 5, CK5, Cytokeratin 5, K5, keratin 5, KRT5, KRT5A, pan-keratin, Type II keratin Kb5

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Galerie de données de validation

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  IF Staining of human colon cancer using CL555-26411

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200.

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  IF Staining of human colon cancer using CL555-26411

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200.

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  IF Staining of human lung cancer using CL555-26411

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200, CoraLite® Plus 488 CD3 antibody (CL488-60181, Clone: 3F3A1, green), CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3, Magenta).

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  IF Staining of human lung cancer using CL555-26411

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200, CoraLite® Plus 488 CD3 antibody (CL488-60181, Clone: 3F3A1, green), CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3, Magenta).

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  IF Staining of A431 using CL555-26411

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200.

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  IF Staining of A431 using CL555-26411

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200.

• 

  IF Staining of human lung cancer using CL555-26411

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200.

• 

  IF Staining of human lung cancer using CL555-26411

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200.

• 

  IF Staining of human breast cancer using CL555-26411

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200.

• 

  IF Staining of human breast cancer using CL555-26411

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200.

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Applications testées

• Résultats positifs en IF: tissu de cancer du côlon humain, cellules A431, tissu de cancer du poumon humain, tissu de cancer du sein humain

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-26411 cible pan-keratin dans les applications de IF et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: pan-keratin Protéine recombinante Ag24184
• Nom complet: keratin 5
• Masse moléculaire calculée: 590 aa, 62 kDa
• Numéro d’acquisition GenBank: BC024292
• Symbole du gène: KRT5
• Identification du gène (NCBI): 3852
• Conjugaison: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers. Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases. This antibody is a pan-keratin antibody.