Anticorps Polyclonal de lapin anti-Gamma Tubulin

Gamma Tubulin Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 26195-1-AP

Synonymes

Gamma 1 tubulin, Gamma Tubulin, GCP 1, TUBG, TUBG1, TUBGCP1, Tubulin gamma 1 chain, tubulin, gamma 1, tubulin-gamma

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Galerie de données de validation

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  WB analysis of mouse brain using 26195-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26195-1-AP (gamma tubulin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26195-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26195-1-AP (gamma tubulin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of C2C12 using 26195-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Gamma Tubulin antibody (26195-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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  IF Staining of C2C12 using 26195-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Gamma Tubulin antibody (26195-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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  IF Staining of MDCK using 26195-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 26195-1-AP (gamma tubulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MDCK using 26195-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 26195-1-AP (gamma tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MDCK using 26195-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 26195-1-AP (gamma tubulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa
• Résultats positifs en IF: cellules C2C12, cellules MDCK

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

26195-1-AP cible Gamma Tubulin dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons canin, Humain, souris

• Réactivité: canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Gamma Tubulin Protéine recombinante Ag23575
• Nom complet: tubulin, gamma 1
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC000619
• Symbole du gène: TUBG1
• Identification du gène (NCBI): 7283
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.