Anticorps Polyclonal de lapin anti-SLC7A11/xCT

• Phare
• Validé par KD/KO

SLC7A11/xCT Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(573)

Avis (5)

N° de cat : 26864-1-AP

Synonymes

SLC7A11, xCT, Amino acid transport system xc, Amino acid transport system xc-, CCBR1

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Galerie de données de validation

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  WB analysis using 26864-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26864-1-AP (SLC7A11/xCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 26864-1-AP

  IP result of anti-SLC7A11/xCT (IP:26864-1-AP, 4ug; Detection:26864-1-AP 1:800) with A549 cells lysate 960 ug.

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  IP experiment of HeLa using 26864-1-AP

  IP result of anti-SLC7A11/xCT (IP:26864-1-AP, 4ug; Detection:26864-1-AP 1:3000) with HeLa cells lysate 1280 ug.

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  IP experiment of HepG2 using 26864-1-AP

  IP result of anti-SLC7A11/xCT (IP:26864-1-AP, 4ug; Detection:26864-1-AP 1:800) with HepG2 cells lysate 1720 ug.

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  IHC staining of human renal cell carcinoma using 26864-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26864-1-AP (SLC7A11/xCT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 26864-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26864-1-AP (SLC7A11/xCT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 26864-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26864-1-AP (SLC7A11/xCT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa non bouillies, cellules HepG2 non bouillies
• Résultats positifs en IP: cellules HeLa, cellules A549, cellules HepG2
• Résultats positifs en IHC: tissu de carcinome à cellules rénales humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 12 publications below
• WB: See 517 publications below
• IHC: See 106 publications below
• IF: See 56 publications below
• IP: See 3 publications below
• CoIP: See 8 publications below
• RIP: See 1 publications below

Informations sur le produit

26864-1-AP cible SLC7A11/xCT dans les applications de WB, IHC, IF, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: bovin, Chèvre, Humain, poulet
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC7A11/xCT Protéine recombinante Ag25431
• Nom complet: solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC012087
• Symbole du gène: SLC7A11
• Identification du gène (NCBI): 23657
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC7A11 (also known as xCT) is a membrane transporter involved in the uptake of cystine. It promotes glutathione synthesis through the uptake of cystine and the concomitant release of glutamate. This, in turn, protects cells from oxidative stress, maintains cellular redox balance, and prevents cell death induced by lipid peroxidation. SLC7A11 has been identified as a potential target for cancer therapeutics. It is overexpressed in multiple malignant tumors and is closely associated with the growth, prognosis, metastasis, and treatment response of various cancers including lung cancer. The SLC7A11 protein appears at two molecular weights, 55 kDa and 35 kDa, and the 55 kDa protein is ubiquitinated(PMID: 32188872). 26864-1-AP recognizes the 35-40 kDa protein similar to the paper published (PMID: 36747082).

Publications

Species	Application	Title
human	WB	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
	WB	Cell Host Microbe; Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice; Authors - Yunong Zeng
human	WB	Drug Resist Updat; Upregulation of CoQ shifts ferroptosis dependence from GPX4 to FSP1 in acquired radioresistance; Authors - Xu Lin
human	WB	Nat Immunol; Glutathione peroxidase 4-regulated neutrophil ferroptosis induces systemic autoimmunity.; Authors - Pengchong Li
human	WB	ACS Nano; Engineering MMP-2 Activated Nanoparticles Carrying B7-H3 Bispecific Antibodies for Ferroptosis-Enhanced Glioblastoma Immunotherapy; Authors - Rangrang Fan
	WB	Nat Commun; Injectable ECM-mimetic dynamic hydrogels abolish ferroptosis-induced post-discectomy herniation through delivering nucleus pulposus progenitor cell-derived exosomes; Authors - Wenkai Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Vignesh (Verified Customer) (09-15-2025)

The results are excellent; bands are clear and loaded 10µg sample.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEPG2A cells

FH

Chloe (Verified Customer) (11-01-2023)

Great antibody works pretty well for a western blot with 1:1000 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Breast Cancer Cell Lines

SLC7A11/xCT Antibody Western Blot validation (1:1000 dilution) in Breast Cancer Cell Lines (Cat no:26864-1-AP)

FH

James (Verified Customer) (11-18-2022)

Detection of SLC7A11 in liver protein extracts

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Liver

SLC7A11/xCT Antibody Western Blot validation (1/1000 dilution) in Liver (Cat no:26864-1-AP)

FH

Melanie (Verified Customer) (05-11-2022)

Tested the antibody in Hek293T cells. Had to dilute the antibody 1:50000, since the signal was to strong. Very clear gang at the calculated MW. Good antibody!

• Applications: Western Blot
• Cell Tissue Type: Hek293T

FH

Amélie (Verified Customer) (05-03-2022)

We used the AB on Bone Marrow derived Macrophages, where Slc7a11 is KO (validate by RT PCR), in Western blot. We did not found a specific staining in the right size.

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: Bone Marrow derived macrophages from WT or KO mice

SLC7A11/xCT Antibody Western Blot validation (1000 dilution) in Bone Marrow derived macrophages from WT or KO mice (Cat no:26864-1-AP)

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