Tested Applications
| Positive WB detected in | PC-12 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18279-1-AP targets PCDHGA1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PCDHGA1 fusion protein Ag13107 Predict reactive species |
| Full Name | protocadherin gamma subfamily A, 1 |
| Calculated Molecular Weight | 823aa,90 kDa; 931aa,101 kDa |
| Observed Molecular Weight | 113 kDa |
| GenBank Accession Number | BC112082 |
| Gene Symbol | PCDHGA1 |
| Gene ID (NCBI) | 56114 |
| RRID | AB_3669297 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y5H4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Protocadherins, which constitute the largest subgroup within the cadherin superfamily, are predominantly expressed in the nervous system and are probably involved in the regulation of neuronal recognition and connectivity (PMID: 17936607; 12231349; 17133224). The protocadherin subfamily can be further subdivided into three groups: the clustered protocadherins, comprising α-, β- and γ-protocadherins; δ-protocadherins; and others, many of which are solitary (PMID: 17133224). PCDHGA1 (protocadherin gamma subfamily A1) is a potential calcium-dependent cell-adhesion protein. It may be involved in establishing and maintaining specific neuronal connections in the brain. PCDHGA1 has four glycosylation sites.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PCDHGA1 antibody 18279-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
