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  • Western Blot (WB) analysis of various lysates using NADK Recombinant antibody (84996-1-RR)

    WB analysis using 84996-1-RR (same clone as 84996-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84996-1-RR (NADK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84996-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84996-1-RR

    Affinity and Kinetic Characterization of 84996-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84996-1-RR against Human NADK were performed. The affinity constant is 2.25 nM.

NADK Recombinant antibody, PBS Only

NADK Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 84996-1-PBS
Clone No.242502B7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

242502B7, EC:2.7.1.23, NAD kinase, Poly(P)/ATP NAD kinase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Product Information

84996-1-PBS targets NADK in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NADK fusion protein Ag7906 Predict reactive species
Full Name NAD kinase
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49-52 kDa
GenBank Accession NumberBC001709
Gene Symbol NADK
Gene ID (NCBI) 65220
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95544
Storage Buffer PBS only, pH 7.3.
Storage ConditionsStore at -80°C.

Background Information

NAD kinase (NADK) is the sole NADP+-biosynthetic enzyme that catalyzes phosphorylation of NAD+ to yield NADP+ using ATP as a phosphoryl donor, and thus, plays a vital role in the cell and represents a potentially powerful antimicrobial drug target (PMID:21526340). It belongs to the NAD kinase family. NADK has 3 isoforms with the molecular mass of 49, 63 and 46 kDa. The catalytically active human NADK is a homotetramer (PMID:11594753).