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Recombinant Human IL-6R alpha protein (Myc Tag, His Tag)

Species

Human

Purity

>95 %, SDS-PAGE

Tag

Myc Tag, His Tag

Activity

EC50: 32-130 ng/mL

Cat no : Eg0180

Validation Data Gallery

  • Purity of Recombinant Human IL-6R alpha was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human IL-6R alpha was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human IL-6 (Myc tag, His tag) at 1 μg/mL (100 μL/well) can bind Human IL-6R alpha (Myc tag, His tag) with a linear range of 32-130 ng/mL.

    Immobilized Human IL-6 (Myc tag, His tag) at 1 μg/mL (100 μL/well) can bind Human IL-6R alpha (Myc tag, His tag) with a linear range of 32-130 ng/mL.

Product Information

Purity >95 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Human IL-6 (Myc tag, His tag) at 1 μg/mL (100 μL/well) can bind Human IL-6R alpha (Myc tag, His tag) with a linear range of 32-130 ng/mL.
Expression HEK293-derived Human IL-6R alpha protein Leu20-Pro365 (Accession# P08887) with a Myc tag and a His tag at the C-terminus.
GeneID 3570
Accession P08887
PredictedSize 41 kDa
SDS-PAGE 60-75 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge. IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor. Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells). Soluble form of IL-6R alpha has been found in human serum and urine.

References:

1. Tilg, H et al. Blood vol. 83,1 (1994): 113-8.
2. Heinrich, P C et al. The Biochemical journal vol. 334 ( Pt 2),Pt 2 (1998): 297-314.
3. Jones, S A et al. FASEB journal : official publication of the Federation of 4. American Societies for Experimental Biology vol. 15,1 (2001): 43-58.
5. Novick, D et al. The Journal of experimental medicine vol. 170,4 (1989): 1409-14. 
6. Honda, M et al. Journal of immunology (Baltimore, Md. : 1950) vol. 148,7 (1992): 2175-80.