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Recombinant Human CSPG4 protein (Myc Tag, His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

Myc Tag, His Tag

Activity

not tested

Cat no : Eg0201

Validation Data Gallery

  • Purity of Recombinant Human CSPG4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CSPG4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human CSPG4 protein Ser1583-Ser2224 (Accession# Q6UVK1) with a Myc tag and a His tag at the C-terminus.
GeneID 1464
Accession Q6UVK1
PredictedSize 73.2 kDa
SDS-PAGE 90-125 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CSPG4, also named as HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16 and NG2, is a proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. CSPG4 may inhibit neurite outgrowth and growth cone collapse during axon regeneration. It is cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. CSPG4 is also a signal-transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. It is a target of specific diagnosis and immunotherapy with antiidiotypic antibodies due, in part, to its roles in growth control, adhesion, cell-substratum interactions, and cell-cell contacts.

References:

1. Matthew A Price, et al. (2011) Pigment Cell Melanoma Res. 24(6):1148-57.
2. J Iida, et al. (2001) J Biol Chem. 276(22):18786-94.
3. Jianbo Yang, et al. (2004) J Cell Biol. 165(6):881-91.
4. K M Eisenmann, et al. (1999) Nat Cell Biol. 1(8):507-13.
5. Valeria Rolih, et al. (2017) J Transl Med. 15(1):151.