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Recombinant Human CEA/CD66e protein (His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0449

Validation Data Gallery

  • Purity of Recombinant Human CEA was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CEA was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human CEA protein Lys35-Ala685 (Accession# NP_004354.2) with a His tag at the C-terminus.
GeneID 1048
Accession NP_004354.2
PredictedSize 75.1 kDa
SDS-PAGE 85-120 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Carcinoembryonic antigen (CEA), also known as CD66e or CEACAM5, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding. CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung. CEA is a tumor marker and is routinely exploited for diagnosis.

References:

1.S Benchimol, et al. (1989) Cell. 57(2):327-34. 
2.Chaogu Zheng, et al. (2011) PLoS One. 6(6):e21146. 
3.Cosme Ordonez, et al. (2007) J Cell Physiol. 210(3):757-65.