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Recombinant Mouse CD14 protein (His Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0847

Validation Data Gallery

  • Purity of Recombinant Mouse CD14 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse CD14 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse CD14 protein Ser16-Pro345 (Accession# P10810) with a His tag at the C-terminus.
GeneID 12475
Accession P10810
PredictedSize 36.5 kDa
SDS-PAGE 42-55 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein. CD14 is preferentially expressed on monocytes and macrophages. CD14 acts as a co-receptor (along with TLR4 and MD-2) for bacterial liposaccharides (LPS). It plays a major role in the inflammatory response of monocytes to LPS. Soluble forms of CD14 (sCD14) have been detected in cell culture supernatants and in human serum and urine, either shedded from the cell surface or released from intracellular pools. Increased release of sCD14 from monocytes is observed after stimulation with various agents, and sCD14 may therefore be a marker for activation of monocytes/macrophages.

References:

1.W A Nockher, et al. (1994) Clin Exp Immunol. 98(3):369-74.
2.A Haziot, et al. (1988) J Immunol. 141(2):547-52.
3.E Lien, et al. (1998) Blood. 92(6):2084-92. 
4.E A Kurt-Jones, et al. (2000) Nat Immunol. 1(5):398-401.