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Recombinant Mouse CD155/PVR protein (His Tag)

Species

Mouse

Purity

>95 %, SDS-PAGE

Tag

His Tag

Activity

EC50: 0.6-3 ng/mL

Cat no : Eg0903

Validation Data Gallery

  • Purity of Recombinant Mouse CD155 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse CD155 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Mouse CD155 (His tag) at 1 μg/mL (100 μL/well) can bind Mouse TIGIT (hFc tag) with a linear range of 0.6-3 ng/mL.

    Immobilized Mouse CD155 (His tag) at 1 μg/mL (100 μL/well) can bind Mouse TIGIT (hFc tag) with a linear range of 0.6-3 ng/mL.

Product Information

Purity >95 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Mouse CD155 (His tag) at 1 μg/mL (100 μL/well) can bind Mouse TIGIT (hFc tag) with a linear range of 0.6-3 ng/mL.
Expression HEK293-derived Mouse CD155 protein Asp29-Leu348 (Accession# Q8K094) with His tag at the C-terminus.
GeneID 52118
Accession Q8K094
PredictedSize 35.6 kDa
SDS-PAGE 48-65 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD155, also known as poliovirus receptor (PVR) or nectin-like protein 5 (NECL-5), is a type I transmembrane glycoprotein which belongs to the nectin and nectin-like family of immunoglobulin superfamily receptors. Mature mouse CD155 consists of a 318 aa extracellular domain, a 24 aa transmembrane segment, and a 38 aa cytoplasmic tail. CD155 plays roles in cell motility, and natural killer and T cell-mediated immunity. It binds to two receptors of NK cells, CD96 and CD226, and accumulates at cell-cell contact sites, leading to the formation of mature immune synapses between NK cells and target cells.

References:

1.Mendelsohn CL. et al. (1989). Cell. 56(5):855-865.
2.Pende D. et al. (2005). Mol Immunol. 42(4):463-469.
3.Fuchs A. et al. (2004). J Immunol. 172(7):3994-8.