• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Recombinant Mouse CCL8/MCP-2 protein (rFc Tag)(HPLC verified)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2771

Validation Data Gallery

  • Purity of Recombinant Mouse CCL8/MCP-2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse CCL8/MCP-2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Mouse CCL8/MCP-2 was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse CCL8/MCP-2 was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse CCL8/MCP-2 protein Gly24-Pro97 (Accession# Q9Z121) with a rabbit IgG Fc tag at the C-terminus.
GeneID 20307
Accession Q9Z121
PredictedSize 34.5 kDa
SDS-PAGE 32-40 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

The cys-cys (C-C) motif chemokine ligand 8 (CCL8; also known as MCP2) is implicated in various inflammatory processes. In bone marrow transplantation, circulating CCL8 levels positively correlate with severity of graft-versus-host disease. In solid organ transplantation, however, the role of CCL8 is less clear.

References:

1. Vilgelm AE. et al. (2019) Front Immunol. 10: 333.
2. Yamamoto M. et al. (2011) Exp Hematol. 39: 1101–1112.
3. Klementowicz JE. et al. (2017) J Immunol. 199: 27–32.
4. Hori T. et al. (2008) Blood. 111: 4403–4412.