Recombinant Human THPO protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg2941
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <1.0 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human THPO protein Ser22-Gly353 (Accession# P40225-1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 7066 |
| Accession | P40225-1 |
| PredictedSize | 61.0 kDa |
| SDS-PAGE | |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Thrombopoietin (TPO) is a primary regulator of megakaryocyte development and platelet production in mammals. Human TPO constitutively circulates and maintains thrombopoiesis through interaction with its cognate receptor, myeloproliferative leukemia protein(MPL). TPO also plays an important role in the maintenance and regulation of hematopoietic stem cells (HSCs). Germline mutations of THPO were reported as causes of hereditary thrombocythemia.
References:
1. Kuter, David J. International journal of hematology vol. 98,1 (2013): 10-23. 2. Kimura, Hiroyuki et al. Experimental hematology vol. 134 (2024): 104208. 3. Kaushansky, K. Journal of thrombosis and haemostasis : JTH vol. 1,7 (2003): 1587-92. 4.Decker, Matthew et al. Science (New York, N.Y.) vol. 360,6384 (2018): 106-110.