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Recombinant Human TL1A/TNFSF15 protein (His Tag)

Species

Human

Purity

>95 %, SDS-PAGE

Tag

His Tag

Activity

EC50: 2-9 ng/mL

Cat no : Eg32092

Validation Data Gallery

  • Purity of Recombinant Human TL1A was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human TL1A was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • Immobilized Human TL1A (His tag) at 0.5 μg/mL (100 μL/well) can bind Human DcR3 (His tag) with a linear range of 2-9 ng/mL.

    Immobilized Human TL1A (His tag) at 0.5 μg/mL (100 μL/well) can bind Human DcR3 (His tag) with a linear range of 2-9 ng/mL.

Product Information

Purity >95 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Human TL1A (His tag) at 0.5 μg/mL (100 μL/well) can bind Human DcR3 (His tag) with a linear range of 2-9 ng/mL.
Expression HEK293-derived Human TL1A protein Leu72-Leu251 (Accession# O95150-1) with a His tag at the N-terminus.
GeneID 9966
Accession O95150-1
PredictedSize 21.3 kDa
SDS-PAGE 25-29 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Tumor necrosis factor ligand superfamily member 15 (TNFSF15) is also named as TL1 and VEGI. And it belongs to the tumor necrosis factor family. It is a receptor for TNFRSF25 and TNFRSF6B. VEGI induced degradation of IκB alpha, and nuclear translocation of p65 subunit of NFκB by activating NFκB. TL1A mediates activation and apoptosis of NFκB in DR3-expressing cell lines. Overexpression by cancer cells of a secretable VEGI fusion protein resulted in abrogation of xenograft tumor progression. The isoforms show endothelial cell-specific expression and are generated from a 17 kb human gene by alternative splicing. In addition, VEGI can inhibit vascular endothelial growth and angiogenesis (in vitro).

References:

1.Haridas V. et al. (1999). Oncogene. 18(47):6496-504. 
2.Migone TS. et al. (2002). Immunity. 16(3):479-92
3. Chew LJ. et al. (2002). FASEB J. 16(7):742-4.
4. Zhai Y. et al. (1999). FASEB J. 13(1):181-9.