Recombinant Human CD80/B7-1 protein (C-rFc)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
C-rFc
Activity
not tested
Cat no : Eg3505
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <1.0 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human CD80/B7-1 protein Val35-Asn242 (Accession# P33681-1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 941 |
| Accession | P33681-1 |
| PredictedSize | 50.1 kDa |
| SDS-PAGE | |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B.
References:
1. Peach, R J et al. The Journal of biological chemistry vol. 270,36 (1995): 21181-7. 2. Vasilevko, Vitaly et al. DNA and cell biology vol. 21,3 (2002): 137-49. 3. Short, Joshua J et al. Virus research vol. 122,1-2 (2006): 144-53.