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Recombinant Human CD55 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg4246

Validation Data Gallery

  • Purity of Recombinant Human CD55 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CD55 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human CD55 protein Asp35-Ser353 (Accession# P08174) with a rabbit IgG Fc tag at the C-terminus.
GeneID 1604
Accession P08174
PredictedSize 61.2 kDa
SDS-PAGE 65-80 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD55, also known as decay-accelerating factor (DAF), is a membrane lipid microstructural domain-associated GPI-anchored protein with a molecular weight ranging from 50 to 100 kDa depending on the cell type. CD55 protects cells from complement-mediated attack by accelerating the decay of C3 and C5, and its absence has been linked to a number of pathologies resulting from over-activation of the complement system. CD55 can signal within cells to promote malignant transformation, cell survival and inhibit apoptosis. CD55 can signal intracellularly to promote malignant transformation, cancer progression, cell survival, angiogenesis, and inhibition of apoptosis. CD55 is enriched in cancer stem cell (CSC) ecosystems in a variety of tumors including breast, ovarian, and cervical cancers and can be induced by chemotherapy and hypoxic environments. (PMID: 36216147)

References:

1. Bharti R,et al. Cancer Lett. 2022;551:215935.                                                         
2. González-Del-Barrio L, et al. FASEB J. 2023;37(11):e23256.                                  
3. Kurolap A, et al. Hum Genet. 2023;142(5):683-690.