SARS-CoV-2 Envelope Protein Polyclonal antibody

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  ELISA experiment of Recombinant protein using 28904-1-AP

  SARS-CoV-2 Envelope protein Antibody (28904-1-AP) tested by ELISA. SARS-CoV-2 Envelope protein was coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of SARS-CoV-2 Envelope protein Antibody (28904-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

SARS-CoV-2 Envelope Protein Polyclonal Antibody for ELISA

Cat No. 28904-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

virus and More (1)

Applications

WB, IF, ELISA

Synonyms

COV19, COV-19, COVID19, COVID-19 E protein, E Protein, envelope protein, SARS-COV-2 E Protein, SARS-CoV-2 Envelope Protein

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Tested Applications

• Positive ELISA detected in: Recombinant protein

Recommended dilution

• Enzyme-linked Immunosorbent Assay (ELISA): ELISA :

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 3 publications below

Product Information

28904-1-AP targets SARS-CoV-2 Envelope Protein in WB, IF, ELISA applications and shows reactivity with virus samples.

• Tested Reactivity: virus
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SARS-CoV-2 Envelope Protein fusion protein Ag30690
• Full Name: COVID-19 E Protein
• GenBank Accession Number: NC_045512
• Gene Symbol: SARS-CoV-2 Envelope Protein
• Gene ID (NCBI): 43740570
• RRID: AB_2881232
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P0DTC4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Envelope protein of coronaviruses is a structural protein existing in both monomeric and homopentameric form. It is a minor component of the virus membrane though it is deemed to be important for many stages including virus infection, replication, dissemination and immune response stimulation. Sequence comparison has shown that this protein is identical to the counterparts of specific Bat and Pangolin coronavirus isolates, even though the Sars-CoV-2 sequence seems to possess specific modifications and characteristics with respect to other Sars CoVs(PMID:32596311). The SARS-CoV-2 envelope protein provide a strategy to assess cross-protection against COVID-19 (PMID: 32446902).

Publications

Species	Application	Title
	WB,IF	bioRxiv; Ubiquitin Ligase ITCH Regulates Life Cycle of SARS-CoV-2 Virus; Authors - Qiwang Xiang
human	IF	iScience; TLR2/NF-κB signaling in macrophage/microglia mediated COVID-pain induced by SARS-CoV-2 envelope protein; Authors - Huan Cui
human	IF	Front Cell Neurosci; The SARS-CoV-2 envelope protein disrupts barrier function in an in vitro human blood-brain barrier model; Authors - Jiahang Ju