4EBP1 Antibody 3 Publications

Mouse Monoclonal| Catalog number: 60246-1-Ig |CloneNo.: 2C3F3

Featured Product KD/KO validated

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Con: 240 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
K-562 cells, K562 , RAW 264.7 cells

Positive IHC detected in:
human pancreas tissue, human colon cancer tissue

Positive IF detected in:
HeLa cells

Recommended dilution:
WB : 1:1000-1:4000
IHC : 1:50-1:500
IF : 1:20-1:200

Product Information


Purification method:
Protein A purification


PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Full name:
eukaryotic translation initiation factor 4E binding protein 1

Calculated molecular weight:
118aa,12 kDa

Observed molecular weight:
15-24 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol

4E BP1, 4EBP1, BP 1, eIF4E binding protein 1, EIF4EBP1, PHAS I

Eukaryotic translation initiation factor 4E-binding protein 1(4EBP1), is a member of 4EBPs family, which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E, thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors, hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1, alpha, beta and gamma.typical pattern seen on Western blots for phosphorylated heat-acid stabled protein 4EBP1 from rat gastrocnemius muscle. Three distinct bands are noted, with the most rapidly migrating band having an apparent molecular mass of ; 20 kDa and the slowest migrating band of; 24 kDa. The 3 bands are designated by convention asalpha, beta and gamma. (PMID: 10913029)

4EBP1 Mouse Monoclonal antibody

Yifei Liao, 11-01-2017

Western Blot
Lot Number

It works well for Western blot

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