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  • KD/KO Validated

4EBP1 Monoclonal antibody

4EBP1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b


human, rat, pig, mouse and More (1)







Cat no : 60246-1-Ig


4E BP1, 4EBP1, BP 1, eIF4E binding protein 1, EIF4EBP1, PHAS I

Tested Applications

Positive WB detected inK-562 cells, RAW 264.7 cells, NCI-H1299 cells, HCT 116 cells, C6 cells, K562
Positive IHC detected inhuman pancreas cancer tissue, mouse liver tissue, rat liver tissue, rat pancreas tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60246-1-Ig targets 4EBP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, pig, mouse samples.

Tested Reactivity human, rat, pig, mouse
Cited Reactivityhuman, chicken
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen 4EBP1 fusion protein Ag18985
Full Name eukaryotic translation initiation factor 4E binding protein 1
Calculated Molecular Weight 118 aa, 12 kDa
Observed Molecular Weight 15-24 kDa
GenBank Accession NumberBC004459
Gene Symbol EIF4EBP1
Gene ID (NCBI) 1978
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eukaryotic translation initiation factor 4E-binding protein 1(4EBP1), is a member of 4EBPs family, which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E, thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors, hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1, alpha, beta and gamma.typical pattern seen on Western blots for phosphorylated heat-acid stabled protein 4EBP1 from rat gastrocnemius muscle. Three distinct bands are noted, with the most rapidly migrating band having an apparent molecular mass of ; 20 kDa and the slowest migrating band of; 24 kDa. The 3 bands are designated by convention asalpha, beta and gamma. (PMID: 10913029)


Product Specific Protocols
WB protocol for 4EBP1 antibody 60246-1-IgDownload protocol
IHC protocol for 4EBP1 antibody 60246-1-IgDownload protocol
IF protocol for 4EBP1 antibody 60246-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.

Authors - Xiao-Yi Lu

J Hepatol

OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.

Authors - Weiqi Dai

Mol Cancer

The circular RNA circ-ERBIN promotes growth and metastasis of colorectal cancer by miR-125a-5p and miR-138-5p/4EBP-1 mediated cap-independent HIF-1α translation.

Authors - Liang-Yan Chen

Cell Death Differ

Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration

Authors - Huaizhen Liang


STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I.

Authors - Cefan Zhou


Toxicity mechanism of peri-implantation pesticide beta-cypermethrin exposure on endometrial remodeling in early pregnant mice

Authors - Yong-Jiang Zhou


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Tom (Verified Customer) (03-02-2021)

10ug of HEK293 lysate resolved on 4-20% gradient acrylamide gel. Membrane blocked (5% BSA) then incubated overnight with antibody (1:2,000). Incubated with secondary HRP antibody for visulization.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HEK293
4EBP1 Antibody Western Blot validation (1:2000 dilution) in HEK293 (Cat no:60246-1-Ig)

Yifei (Verified Customer) (11-01-2017)

It works well for Western blot

  • Applications: Western Blot