5-Formylcytosine (5-fC) antibody (pAb)
Host / Isotype
Rabbit / Serum
Reactivity
Not Species Specific
Applications
DB, ICC, IF, IHC, WB
Cat No : 61223,61224 61223
Synonyms
Validation Data Gallery
Product Information
Tested Applications |
DB, ICC, IF, IHC, WB
Applications Validated by Active Motif: DB: 1:5,000 dilution |
Tested Reactivity | Not Species Specific |
Host / Isotype | Rabbit / Serum |
Class | Polyclonal |
Type | Antibody |
Modification | Methylated |
Immunogen | This 5-Formylcytosine antibody was raised against 5-formylcytidine conjugated to KLH and recognizes 5-formylcytosine. |
Full Name | 5-Formylcytosine (5-fC) antibody (pAb) |
Synonyms | 5-Formylcytidine, 5-formylcytosine, 5-fC, 5fc, TET, dna methylation, stem cell, stem cells, embryogenesis, sample |
Molecular weight | |
GenBank accession number | N/A |
RRID | AB_2687953 |
Purification Method | None |
Buffer | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC into 5-formylcytosine and 5-carboxylcytosine. An increase in levels of 5-formylcytosine and 5-carboxylcytosine are detected in the mouse male pronucleus following fertilization, which is gradually diluted by DNA replication.