5-Hydroxymethylcytosine (5-hmC) antibody (mAb) (Clone 59.1)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Not Species Specific

Applications

DB, ICC, IF, IHC, MeDIP

CloneNo.

59.1

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Cat No : 39999,40000,40900 39999

Synonyms

5-Hydroxymethylcytidine, 5-hydroxymethylcytosine, 5-hmC, 5-Hydroxy methylcytidine, 5-hydroxy methyl cytidine, 5-hydroxymethylcytosine, 5-hmc, 5 hmc, 5hmc, hydroxy methyl cytosine, Hydroxymethylcytosine, Hydroxy methylcytosine, Hydroxy methyl cytosine, monoclonal, antibody, antibodies, mAb, epigenetics, epigenetic, hydroxy methyl cytosine, hydroxymethyl, 5-hydroxy-methyl-cytidine, hydroxy-methyl-cytosine, hydroxy-methylcytidine, DNA methylation, clone 59.1, medip, sample



Product Information

Tested Applications DB, ICC, IF, IHC, MeDIP

Applications Validated by Active Motif: MeDIP: 1 - 2 ug per IP MeDIP-chip: 10 ug per IP DB: 0.2 mg/ml dilution

Tested Reactivity Human, Mouse, Not Species Specific
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
ModificationMethylated
Immunogen This 5-Hydroxymethylcytosine antibody was raised against 5-hydroxymethylcytidine conjugated to KLH and recognizes 5-hydroxymethylcytosine.
Full Name 5-Hydroxymethylcytosine (5-hmC) antibody (mAb) (Clone 59.1)
Synonyms 5-Hydroxymethylcytidine, 5-hydroxymethylcytosine, 5-hmC, 5-Hydroxy methylcytidine, 5-hydroxy methyl cytidine, 5-hydroxymethylcytosine, 5-hmc, 5 hmc, 5hmc, hydroxy methyl cytosine, Hydroxymethylcytosine, Hydroxy methylcytosine, Hydroxy methyl cytosine, monoclonal, antibody, antibodies, mAb, epigenetics, epigenetic, hydroxy methyl cytosine, hydroxymethyl, 5-hydroxy-methyl-cytidine, hydroxy-methyl-cytosine, hydroxy-methylcytidine, DNA methylation, clone 59.1, medip, sample
Molecular weight
GenBank accession numberN/A
RRIDAB_2566808
Purification Method Protein G Chromatography
Buffer Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

In addition to this monoclonal, Active Motif offers two polyclonal antibodies that recognize 5-hydroxymethylcytosine, a whole serum version (39769) and a purified IgG version (39791).All are validated for use in methyl DNA immunoprecipitation (MeDIP). For customers that require the ability to quantitate the amount of IgG in the MeDIP reaction, we recommend either this monoclonal or the purified IgG polyclonal (39791). The whole serum version (39769) is very high titre and should be used carefully (0.1 - 0.5 ul per IP) as not to generate high non-specific background. The whole serum version (39769) has been used successfully in immunofluorescence (IF, Ito et al, 2010), and the purified IgG version (39791) is likely to work in this application as well. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. This antibody was developed specifically to distinguish 5-hydroxymethylcytosine from 5-methylcytosine as conventional methods (enrichment by antibody or methyl DNA binding protein, enzymatic digestion and bisulfite sequencing) cannot do so. It is possible that 5-hydroxymethylcytosine (5-hmC) represents a pathway to demethylate DNA, as 5-hydroxymethylcytosine is repaired as mismatched DNA and replaced with unmethylated cytosine.