5-Hydroxymethylcytosine (5-hmC) antibody (pAb)

Host / Isotype

Rabbit / Serum

Reactivity

Human, Mouse, Not Species Specific

Applications

DB, FC, ICC, IF, IHC, MeDIP

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Cat No : 39069,39769,39770 39769

Synonyms

5-Hydroxymethylcytidine, 5-hydroxymethylcytosine, 5-hmC, 5-Hydroxy methylcytidine, 5-hydroxy methyl cytidine, 5-hydroxymethylcytosine, 5-hmc, 5 hmc, 5hmc, hydroxy methyl cytosine, Hydroxymethylcytosine, Hydroxy methylcytosine, Hydroxy methyl cytosine, polyclonal, antibody, antibodies, pAb, epigenetics, epigenetic, hydroxy methyl cytosine, hydroxymethyl, 5-hydroxy-methyl-cytidine, hydroxy-methyl-cytosine, hydroxy-methylcytidine, DNA methylation, medip, sample



Product Information

Tested Applications DB, FC, ICC, IF, IHC, MeDIP

Applications Validated by Active Motif: MeDIP: 0.1 - 0.5 ul per IP DB: 1:10,000 dilution

Tested Reactivity Human, Mouse, Not Species Specific
Host / Isotype Rabbit / Serum
Class Polyclonal
Type Antibody
ModificationMethylated
Immunogen This 5-Hydroxymethylcytosine antibody was raised against 5-hydroxymethylcytidine conjugated to KLH and recognizes 5-hydroxymethylcytosine.
Full Name 5-Hydroxymethylcytosine (5-hmC) antibody (pAb)
Synonyms 5-Hydroxymethylcytidine, 5-hydroxymethylcytosine, 5-hmC, 5-Hydroxy methylcytidine, 5-hydroxy methyl cytidine, 5-hydroxymethylcytosine, 5-hmc, 5 hmc, 5hmc, hydroxy methyl cytosine, Hydroxymethylcytosine, Hydroxy methylcytosine, Hydroxy methyl cytosine, polyclonal, antibody, antibodies, pAb, epigenetics, epigenetic, hydroxy methyl cytosine, hydroxymethyl, 5-hydroxy-methyl-cytidine, hydroxy-methyl-cytosine, hydroxy-methylcytidine, DNA methylation, medip, sample
Molecular weight
GenBank accession numberN/A
RRIDAB_10013602
Purification Method None
Buffer Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Active Motif offers two polyclonal antibodies that recognize 5-hydroxymethylcytosine, a whole serum version (39769) and a purified IgG version (39791). Both are validated for use in methyl DNA immunoprecipitation (MeDIP). For customers who must quantitate the amount of IgG in the MeDIP reaction, the purified IgG version (39791) is recommended. The whole serum version (39769) is very high titre, so should be used carefully (0.1 - 0.5 ul per IP) to prevent non-specific background. The whole serum version (39769) has been used successfully in immunofluorescence (IF, Ito et al, 2010);the purified IgG version (39791) is likely to work in IF as well. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. This antibody was developed specifically to distinguish 5-hydroxymethylcytosine from 5-methylcytosine as conventional methods (enrichment by antibody or methyl DNA binding protein, enzymatic digestion and bisulfite sequencing) cannot do so. It is possible that 5-hydroxymethylcytosine (5-hmC) represents a pathway to demethylate DNA, as 5-hydroxymethylcytosine is repaired as mismatched DNA and replaced with unmethylated cytosine.