5-Methylcytosine (5-mC) antibody (mAb) (Clone A1)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Not Species Specific

Applications

DB, IF, MeDIP

CloneNo.

A1

Activ Motif logo

Cat No : 61479,61480 61479

Synonyms

5-methylcytidine, methyl cytidine, 5 MeCyd,5-mC, 5mC, 5mc, 5-Me, 5 MeC, MeDIP, methylated DNA immunoprecipitation, DNA methylation, monoclonal, mouse, mAb, antibody, antibodies, methylcytosine, stem cell, stem cells, sample



Product Information

Tested Applications DB, IF, MeDIP

Applications Validated by Active Motif: MeDIP: 1 - 2 ug per IP MeDIP-Seq: 1 - 2 ug each DB: 0.5 - 2 ug/ml dilution In MeDIP, the DNA must be denatured when using this antibody. For 5-methylcytosine, we also offer AbFlex 5-methylcytosine Recombinant Antibody (rAb). For details, see Catalog No. 91187.

Tested Reactivity Human, Not Species Specific
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
ModificationMethylated
Immunogen This 5-Methylcytosine (5-mC) antibody was raised against 5-Methyl-cytidine conjugated to KLH and recognizes 5-Methylcytosine.
Full Name 5-Methylcytosine (5-mC) antibody (mAb) (Clone A1)
Synonyms 5-methylcytidine, methyl cytidine, 5 MeCyd,5-mC, 5mC, 5mc, 5-Me, 5 MeC, MeDIP, methylated DNA immunoprecipitation, DNA methylation, monoclonal, mouse, mAb, antibody, antibodies, methylcytosine, stem cell, stem cells, sample
Molecular weight
GenBank accession numberN/A
RRIDAB_2793653
Purification Method Protein A Chromatography
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

5-Methylcytosine (5-mC) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. The 5-methylcytidine antibody (Clone A1) has been developed to discriminate between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis.