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AACS Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 13815-1-AP

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Con: 70 μg/150 μl

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Tested Applications

Applications:
WB, IF, ELISA
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Images See all

AACS Antibody WB mouse heart tissue 13815-1-AP

mouse heart tissue were subjected to SDS PAGE followed by western blot with 13815-1-AP( AACS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

AACS Antibody IF MCF-7 cells 13815-1-AP

Immunofluorescent analysis of MCF-7 cells using 13815-1-AP(AACS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse heart tissue, mouse liver tissue
Positive IF detected in:
MCF-7 cells
Recommended dilution:
WB : 1:200-1:1000
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
AACS fusion protein Ag4899
Full name:
acetoacetyl-CoA synthetase
Calculated molecular weight:
672aa,75 kDa
Observed molecular weight:
67 kDa, 30 kDa
GenBank accession number:
BC040490
Gene ID (NCBI):
65985
Gene symbol
AACS
Synonyms
AACS, acetoacetyl CoA synthetase, ACSF1, Protein sur 5 homolog, SUR 5

Background

AACS(acetoacetyl-CoA synthetase) is also named as ACSF1 and belonogs to the ATP-dependent AMP-binding enzyme family. In mammals, AACS is present in the cytosolic fractions of various tissues, especially lipogenic ones, and acetoacetate is known to be effectively incorporated into cholesterol and fatty acids. These results indicate that AACS may supply acetyl units to cytosolic compartment for cholesterol and/or fatty acid biosynthesis. It has 3 isoforms produced by alternative splicing.


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