AACS Polyclonal antibody

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  WB analysis of mouse heart using 13815-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13815-1-AP (AACS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 13815-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13815-1-AP (AACS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 13815-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13815-1-AP (AACS antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 13815-1-AP

  Immunofluorescent analysis of MCF-7 cells using 13815-1-AP (AACS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

AACS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13815-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Protein sur 5 homolog, EC:6.2.1.16, Acyl-CoA synthetase family member 1, ACSF1, ACSF 1

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse liver tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

13815-1-AP targets AACS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AACS fusion protein Ag4899
• Full Name: acetoacetyl-CoA synthetase
• Calculated Molecular Weight: 672 aa, 75 kDa
• Observed Molecular Weight: 67 kDa, 30 kDa
• GenBank Accession Number: BC040490
• Gene Symbol: AACS
• Gene ID (NCBI): 65985
• RRID: AB_2877977
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86V21
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AACS(acetoacetyl-CoA synthetase) is also named as ACSF1 and belonogs to the ATP-dependent AMP-binding enzyme family. In mammals, AACS is present in the cytosolic fractions of various tissues, especially lipogenic ones, and acetoacetate is known to be effectively incorporated into cholesterol and fatty acids. These results indicate that AACS may supply acetyl units to cytosolic compartment for cholesterol and/or fatty acid biosynthesis. It has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	Sci Adv; Ketogenesis supports hepatic polyunsaturated fatty acid homeostasis via fatty acid elongation; Authors - Eric D Queathem
mouse	WB	iScience; Hepatocyte activity of the cholesterol sensor smoothened regulates cholesterol and bile acid homeostasis in mice.; Authors - George D Dalton
mouse	WB	bioRxiv; Ketogenesis supports hepatic polyunsaturated fatty acid homeostasis via fatty acid elongation; Authors - Eric D Queathem
mouse	WB	bioRxiv; An alternative route for β-hydroxybutyrate metabolism supports fatty acid synthesis in cancer cells; Authors - Faith C Kaluba ; KD Validated