AACS Polyclonal antibody

AACS Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 13815-1-AP

Synonyms

AACS, acetoacetyl CoA synthetase, ACSF1, Protein sur 5 homolog, SUR 5



Tested Applications

Positive WB detected inmouse heart tissue, mouse liver tissue
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13815-1-AP targets AACS in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AACS fusion protein Ag4899
Full Name acetoacetyl-CoA synthetase
Calculated molecular weight 672 aa, 75 kDa
Observed molecular weight 67 kDa, 30 kDa
GenBank accession numberBC040490
Gene symbol AACS
Gene ID (NCBI) 65985
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AACS(acetoacetyl-CoA synthetase) is also named as ACSF1 and belonogs to the ATP-dependent AMP-binding enzyme family. In mammals, AACS is present in the cytosolic fractions of various tissues, especially lipogenic ones, and acetoacetate is known to be effectively incorporated into cholesterol and fatty acids. These results indicate that AACS may supply acetyl units to cytosolic compartment for cholesterol and/or fatty acid biosynthesis. It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for AACS antibody 13815-1-APDownload protocol
IF protocol for AACS antibody 13815-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols