AACS Polyclonal antibody

AACS Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 13815-1-AP

Synonyms

AACS, acetoacetyl CoA synthetase, ACSF1, Protein sur 5 homolog, SUR 5

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Validation Data Gallery

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  WB analysis of mouse heart using 13815-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13815-1-AP (AACS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 13815-1-AP

  Immunofluorescent analysis of MCF-7 cells using 13815-1-AP (AACS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse liver tissue
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Product Information

13815-1-AP targets AACS in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AACS fusion protein Ag4899
• Full Name: acetoacetyl-CoA synthetase
• Calculated molecular weight: 672 aa, 75 kDa
• Observed molecular weight: 67 kDa, 30 kDa
• GenBank accession number: BC040490
• Gene symbol: AACS
• Gene ID (NCBI): 65985
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

AACS(acetoacetyl-CoA synthetase) is also named as ACSF1 and belonogs to the ATP-dependent AMP-binding enzyme family. In mammals, AACS is present in the cytosolic fractions of various tissues, especially lipogenic ones, and acetoacetate is known to be effectively incorporated into cholesterol and fatty acids. These results indicate that AACS may supply acetyl units to cytosolic compartment for cholesterol and/or fatty acid biosynthesis. It has 3 isoforms produced by alternative splicing.