AACS Polyclonal antibody
AACS Polyclonal Antibody for IF, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF,ELISA
Conjugate
Unconjugated
Cat no : 13815-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse heart tissue, mouse liver tissue |
Positive IF detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:200-1:1000 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Product Information
13815-1-AP targets AACS in WB, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | AACS fusion protein Ag4899 |
Full Name | acetoacetyl-CoA synthetase |
Calculated molecular weight | 672 aa, 75 kDa |
Observed molecular weight | 67 kDa, 30 kDa |
GenBank accession number | BC040490 |
Gene symbol | AACS |
Gene ID (NCBI) | 65985 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AACS(acetoacetyl-CoA synthetase) is also named as ACSF1 and belonogs to the ATP-dependent AMP-binding enzyme family. In mammals, AACS is present in the cytosolic fractions of various tissues, especially lipogenic ones, and acetoacetate is known to be effectively incorporated into cholesterol and fatty acids. These results indicate that AACS may supply acetyl units to cytosolic compartment for cholesterol and/or fatty acid biosynthesis. It has 3 isoforms produced by alternative splicing.
Protocols
Product Specific Protocols | |
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WB protocol for AACS antibody 13815-1-AP | Download protocol |
IF protocol for AACS antibody 13815-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |