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ABCG1 Antibody

ABCG1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 13578-1-AP

Synonyms

ABC8, ABCG1, White protein homolog, WHITE1, WHT1



Tested Applications

Positive WB detected inHeLa cells, RAW 264.7 cells, HepG2 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman cervical cancer tissue, human tonsillitis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13578-1-AP targets ABCG1 in IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABCG1 fusion protein Ag4479
Full Name ATP-binding cassette, sub-family G (WHITE), member 1
Calculated molecular weight 678aa,76 kDa
Observed molecular weight 90-100 kDa
GenBank accession number BC029158
Gene symbol ABCG1
Gene ID (NCBI) 9619
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

ABCG1 is a cholesterol and phospholipid transporter expressed in lung, brain, spleen, and macrophages. In liver, ABCG1 is expressed mainly in Kupffer cells. The monomer of ABCG1 is around 60-65 kDa, while multiple forms of ABCG1 can be observed on SDS-PAGE, including 130 kDa (dimer), 90-95 kDa, 110 kDa. It was not clear whether the glycosylation or splicing accounts for the differences in mass. (PMID: 16702602)

Protocols

Product Specific Protocols
WB protocol for ABCG1 antibody 13578-1-APDownload protocol
IHC protocol for ABCG1 antibody 13578-1-APDownload protocol
IP protocol for ABCG1 antibody 13578-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biochem Biophys Res Commun

ALDH2 deficiency inhibits Ox-LDL induced foam cell formation via suppressing CD36 expression.

Authors - Shujian Wei
mouseWB

Environ Int

Low-dose PCB126 compromises circadian rhythms associated with disordered glucose and lipid metabolism in mice.

Authors - Xinming Shen
humanWB

Signal Transduct Target Ther

The circFASN/miR-33a pathway participates in tacrolimus-induced dysregulation of hepatic triglyceride homeostasis.

Authors - Chenzhi Zhang
mouseWB

Biochim Biophys Acta

DNA topoisomerase II inhibitors induce macrophage ABCA1 expression and cholesterol efflux-an LXR-dependent mechanism.

Authors - Zhang Ling L
humanWB

Br J Pharmacol

Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy.

Authors - Chuanrui Ma
humanWB

Arch Med Sci

Effects of Porphyromonas gingivalis lipopolysaccharide on the expression of key genes involved in cholesterol metabolism in macrophages.

Authors - Fen Liu