- Featured Product
- KD/KO Validated
ABCG1 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
IHC, IP, WB, ELISA
Cat no : 13578-1-AP
ABC8, ABCG1, White protein homolog, WHITE1, WHT1
|Positive WB detected in||HeLa cells, RAW 264.7 cells, HepG2 cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human cervical cancer tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
13578-1-AP targets ABCG1 in IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.
|Reactivity||human, mouse, rat|
|Cited Species||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ABCG1 fusion protein Ag4479|
|Full Name||ATP-binding cassette, sub-family G (WHITE), member 1|
|Calculated molecular weight||678aa,76 kDa|
|Observed molecular weight||90-100 kDa|
|GenBank accession number||BC029158|
|Gene ID (NCBI)||9619|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20oC. Aliquoting is unnecessary for -20oC storage.|
ABCG1 is a cholesterol and phospholipid transporter expressed in lung, brain, spleen, and macrophages. In liver, ABCG1 is expressed mainly in Kupffer cells. The monomer of ABCG1 is around 60-65 kDa, while multiple forms of ABCG1 can be observed on SDS-PAGE, including 130 kDa (dimer), 90-95 kDa, 110 kDa. It was not clear whether the glycosylation or splicing accounts for the differences in mass. (PMID: 16702602)
Biochem Biophys Res Commun
ALDH2 deficiency inhibits Ox-LDL induced foam cell formation via suppressing CD36 expression.
Low-dose PCB126 compromises circadian rhythms associated with disordered glucose and lipid metabolism in mice.
Signal Transduct Target Ther
The circFASN/miR-33a pathway participates in tacrolimus-induced dysregulation of hepatic triglyceride homeostasis.
Biochim Biophys Acta
DNA topoisomerase II inhibitors induce macrophage ABCA1 expression and cholesterol efflux-an LXR-dependent mechanism.
Br J Pharmacol
Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy.
Arch Med Sci
Effects of Porphyromonas gingivalis lipopolysaccharide on the expression of key genes involved in cholesterol metabolism in macrophages.