ABCG1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ABCG1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

28

Cat no : 13578-1-AP

Synonyms

ABC8, ABCG1, White protein homolog, WHITE1, WHT1

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Validation Data Gallery

• 

  WB analysis of HeLa using 13578-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13578-1-AP (ABCG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 13578-1-AP

  WB result of ABCG1 antibody (13578-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABCG1 transfected RAW 264.7 cells.

• 

  WB analysis of HepG2 using 13578-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13578-1-AP (ABCG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 13578-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 13578-1-AP (ABCG1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13578-1-AP

  IP Result of anti-ABCG1 (IP:13578-1-AP, 4ug; Detection:13578-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human cervical cancer using 13578-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13578-1-AP (ABCG1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 13578-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13578-1-AP (ABCG1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human tonsillitis using 13578-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13578-1-AP (ABCG1 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: HeLa cells, RAW 264.7 cells, HepG2 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human cervical cancer tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 26 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

13578-1-AP targets ABCG1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABCG1 fusion protein Ag4479
• Full Name: ATP-binding cassette, sub-family G (WHITE), member 1
• Calculated molecular weight: 678 aa, 76 kDa
• Observed molecular weight: 90-100 kDa
• GenBank accession number: BC029158
• Gene symbol: ABCG1
• Gene ID (NCBI): 9619
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ABCG1 is a cholesterol and phospholipid transporter expressed in lung, brain, spleen, and macrophages. In liver, ABCG1 is expressed mainly in Kupffer cells. The monomer of ABCG1 is around 60-65 kDa, while multiple forms of ABCG1 can be observed on SDS-PAGE, including 130 kDa (dimer), 90-95 kDa, 110 kDa. It was not clear whether the glycosylation or splicing accounts for the differences in mass. (PMID: 16702602)

Publications

Species	Application	Title
mouse	WB	Biochem Biophys Res Commun; ALDH2 deficiency inhibits Ox-LDL induced foam cell formation via suppressing CD36 expression.; Authors - Shujian Wei
mouse	WB	Environ Int; Low-dose PCB126 compromises circadian rhythms associated with disordered glucose and lipid metabolism in mice.; Authors - Xinming Shen
human	WB	Signal Transduct Target Ther; The circFASN/miR-33a pathway participates in tacrolimus-induced dysregulation of hepatic triglyceride homeostasis.; Authors - Chenzhi Zhang
mouse	WB	Biochim Biophys Acta; DNA topoisomerase II inhibitors induce macrophage ABCA1 expression and cholesterol efflux-an LXR-dependent mechanism.; Authors - Zhang Ling L
human	WB	Br J Pharmacol; Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy.; Authors - Chuanrui Ma
human	WB	Signal Transduct Target Ther; The circFASN/miR-33a pathway participates in tacrolimus-induced dysregulation of hepatic triglyceride homeostasis.; Authors - Chenzhi Zhang

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