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- KD/KO Validated
ABCG1 Polyclonal antibody
ABCG1 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 13578-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, RAW 264.7 cells, HepG2 cells |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human cervical cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 26 publications below |
IHC | See 3 publications below |
IF | See 3 publications below |
Product Information
13578-1-AP targets ABCG1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ABCG1 fusion protein Ag4479 |
Full Name | ATP-binding cassette, sub-family G (WHITE), member 1 |
Calculated molecular weight | 678 aa, 76 kDa |
Observed molecular weight | 90-100 kDa |
GenBank accession number | BC029158 |
Gene symbol | ABCG1 |
Gene ID (NCBI) | 9619 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ABCG1 is a cholesterol and phospholipid transporter expressed in lung, brain, spleen, and macrophages. In liver, ABCG1 is expressed mainly in Kupffer cells. The monomer of ABCG1 is around 60-65 kDa, while multiple forms of ABCG1 can be observed on SDS-PAGE, including 130 kDa (dimer), 90-95 kDa, 110 kDa. It was not clear whether the glycosylation or splicing accounts for the differences in mass. (PMID: 16702602)
Protocols
Product Specific Protocols | |
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WB protocol for ABCG1 antibody 13578-1-AP | Download protocol |
IHC protocol for ABCG1 antibody 13578-1-AP | Download protocol |
IP protocol for ABCG1 antibody 13578-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biochem Biophys Res Commun ALDH2 deficiency inhibits Ox-LDL induced foam cell formation via suppressing CD36 expression. | ||
Environ Int Low-dose PCB126 compromises circadian rhythms associated with disordered glucose and lipid metabolism in mice. | ||
Signal Transduct Target Ther The circFASN/miR-33a pathway participates in tacrolimus-induced dysregulation of hepatic triglyceride homeostasis. | ||
Biochim Biophys Acta DNA topoisomerase II inhibitors induce macrophage ABCA1 expression and cholesterol efflux-an LXR-dependent mechanism. | ||
Br J Pharmacol Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy. | ||
Signal Transduct Target Ther The circFASN/miR-33a pathway participates in tacrolimus-induced dysregulation of hepatic triglyceride homeostasis. |