Name: ABCG1 Rabbit Polyclonal antibody
Brand: Proteintech

Tested Applications

Applications:

WB, IP, IHC, IF, ELISA
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Images See all

ABCG1 Antibody WB multi-cells/tissue 13578-1-AP

WB result of 13578-1-ap(ABCG1 antibody) on multiple lysates.

ABCG1 Antibody IHC human cervical cancer tissue 13578-1-AP

Immunohistochemical of paraffin-embedded human cervical cancer using 13578-1-AP(ABCG1 antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human cervical cancer using 13578-1-AP(ABCG1 antibody) at dilution of 1:100 (under 40x lens)

ABCG1 Antibody IP mouse brain tissue 13578-1-AP

IP Result of anti-ABCG1 (IP:13578-1-AP, 4ug; Detection:13578-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

Species specificity:

human, mouse, rat

Positive WB detected in:

multi-cells/tissue, HeLa cells, Jurkat cells, mouse brain tissue, mouse lung tissue

Positive IP detected in:

mouse brain tissue

Positive IHC detected in:

human cervical cancer tissue, human tonsillitis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution:

WB : 1:500-1:2000

IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

IHC : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

ABCG1 fusion protein Ag4479

Full name:

ATP-binding cassette, sub-family G (WHITE), member 1

Calculated molecular weight:

678aa,76 kDa

Observed molecular weight:

60-65 kDa

GenBank accession number:

BC029158

Gene ID (NCBI):

9619

Gene symbol

ABCG1

Synonyms

ABC8, ABCG1, White protein homolog, WHITE1, WHT1

Background

ABCG1 is a cholesterol and phospholipid transporter expressed in lung, brain, spleen, and macrophages. In liver, ABCG1 is expressed mainly in Kupffer cells. The monomer of ABCG1 is around 60-65 kDa, while multiple forms of ABCG1 can be observed on SDS-PAGE, including 130 kDa (dimer), 90-95 kDa, 110 kDa. It was not clear whether the glycosylation or splicing accounts for the differences in mass. (PMID: 16702602)

Showing 1- [6] of [10] Publications

Author:		Xue Jiahong J
Pubmed ID:		23693076
Journal:		Diabetes Res Clin Pract
Title:		ATP-binding cassette transporter G1 protects against endothelial dysfunction induced by high glucose.
Application:		WB
KD validated
Species:		human

Author:		Xue Jiahong J
Pubmed ID:		23897420
Journal:		J Physiol Sci
Title:		ABCG1 deficiency promotes endothelial apoptosis by endoplasmic reticulum stress-dependent pathway.
Application:

Species:

Author:		Shuangying Hao
Pubmed ID:		26633327
Journal:		Molecules
Title:		Mitochondrion-Targeted Peptide SS-31 Inhibited Oxidized Low-Density Lipoproteins-Induced Foam Cell Formation through both ROS Scavenging and Inhibition of Cholesterol Influx in RAW264.7 Cells.
Application:		WB

Species:		human

Author:		Ling Zhang
Pubmed ID:		27365310
Journal:		Biochim Biophys Acta
Title:		MEK1/2 inhibitors activate macrophage ABCG1 expression and reverse cholesterol transport-An anti-atherogenic function of ERK1/2 inhibition.
Application:		WB,IF

Species:		mouse

Author:		Fen Liu
Pubmed ID:		27695485
Journal:		Arch Med Sci
Title:		Effects of Porphyromonas gingivalis lipopolysaccharide on the expression of key genes involved in cholesterol metabolism in macrophages.
Application:		WB

Species:		human

Author:		Ying Liu
Pubmed ID:		29298812
Journal:		J Lipid Res
Title:		25-Hydroxycholesterol activates the expression of cholesterol 25-hydroxylase in an LXR-dependent mechanism.
Application:		WB

Species:		human

WB Figures

HeLa cells were subjected to SDS PAGE followed by western blot with 13578-1-AP(ABCG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 13578-1-AP(ABCG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

ABCG1 Antibody WB Jurkat cells 13578-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 13578-1-AP(ABCG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

ABCG1 Antibody WB mouse brain tissue 13578-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 13578-1-AP(ABCG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

ABCG1 Antibody WB mouse lung tissue 13578-1-AP

mouse lung tissue were subjected to SDS PAGE followed by western blot with 13578-1-AP(ABCG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

WB result of 13578-1-ap(ABCG1 antibody) on multiple lysates.

RAW264.7 cells were subjected to SDS PAGE followed by western blot with 13578-1-AP(ABCG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemical of paraffin-embedded human cervical cancer using 13578-1-AP(ABCG1 antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human cervical cancer using 13578-1-AP(ABCG1 antibody) at dilution of 1:100 (under 40x lens)

ABCG1 Antibody IHC human tonsillitis tissue 13578-1-AP

Immunohistochemistry of paraffin-embedded human tonsillitis slide using 13578-1-AP( ABCG1 Antibody) at dilution of 1:50

IP Figures

IP Result of anti-ABCG1 (IP:13578-1-AP, 4ug; Detection:13578-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

ABCG1 Antibody 10 Publications

Rabbit Polyclonal| Catalog number: 13578-1-AP

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background

Showing 1- [6] of [10] Publications

WB Figures

IHC Figures

IP Figures

ABCG1 Antibody 10 Publications

Rabbit Polyclonal| Catalog number: 13578-1-AP

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Related Primary Antibodies

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background