Tested Applications
| Positive WB detected in | HUVEC cells, HeLa cells, HepG2 cells, mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | mouse placenta tissue, human tonsillitis tissue, rat pancreas tissue, human placenta tissue, mouse kidney tissue, human cervical cancer tissue, mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13578-1-AP targets ABCG1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4479 Product name: Recombinant human ABCG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-353 aa of BC029158 Sequence: MACLMAAFSVGTAMNASSYSAEMTEPKSVCVSVDEVVSSNMEATETDLLNGHLKKVDNNLTEAQRFSSLPRRAAVNIEFRDLSYSVPEGPWWRKKGYKTLLKGISGKFNSGELVAIMGPSGAGKSTLMNILAGYRETGMKGAVLINGLPRDLRCFRKVSCYIMQDDMLLPHLTVQEAMMVSAHLKLQEKDEGRREMVKEILTALGLLSCANTRTGSLSGGQRKRLAIALELVNNPPVMFFDEPTSGLDSASCFQVVSLMKGLAQGGRSIICTIHQPSAKLFELFDQLYVLSQGQCVYRGKVCNLVPYLRDLGLNCPTYHNPADFVMEVASGEYGDQNSRLVRAVREGMCDSDH Predict reactive species |
| Full Name | ATP-binding cassette, sub-family G (WHITE), member 1 |
| Calculated Molecular Weight | 678 aa, 76 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC029158 |
| Gene Symbol | ABCG1 |
| Gene ID (NCBI) | 9619 |
| RRID | AB_2220174 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P45844 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ABCG1 is a cholesterol and phospholipid transporter expressed in lung, brain, spleen, and macrophages. In liver, ABCG1 is expressed mainly in Kupffer cells. The monomer of ABCG1 is around 60-65 kDa, while multiple forms of ABCG1 can be observed on SDS-PAGE, including 130 kDa (dimer), 90-95 kDa, 110 kDa. It was not clear whether the glycosylation or splicing accounts for the differences in mass. (PMID: 16702602)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ABCG1 antibody 13578-1-AP | Download protocol |
| WB protocol for ABCG1 antibody 13578-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ABCG1 antibody (Cat# 13578-1-AP) has 93 citations. These appear across journals including Nature Communications, Cancer Research, Autophagy, Theranostics, Acta Pharmaceutica Sinica B, British Journal of Pharmacology, Cell Death & Disease, Journal of Nanobiotechnology, Molecules, iScience, and Frontiers in Pharmacology. Research fields are dominated by atherosclerosis, cholesterol/lipid metabolism, and macrophage foam cell formation, with additional work in cancer, NAFLD, and neurology. Applications are mainly Western blot, with some IHC and IF.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther The circFASN/miR-33a pathway participates in tacrolimus-induced dysregulation of hepatic triglyceride homeostasis. | ||
Cancer Res ANO1 reprograms cholesterol metabolism and the tumor microenvironment to promote cancer metastasis | ||
Cancer Res PRMT1 sustains de novo fatty acid synthesis by methylating PHGDH to drive chemoresistance in triple-negative breast cancer | ||
Autophagy Metabolic effects of RUBCN/Rubicon deficiency in kidney proximal tubular epithelial cells. | ||
Nat Commun Cyclic fasting bolsters cholesterol biosynthesis inhibitors' anticancer activity | ||
J Adv Res Plasma apolipoprotein E protein attenuates pulmonary fibrosis through LRP1 and PLAU dual receptor-mediated TGF-β/Smad inhibition. |
Reviews
What customers say
The single review rates the product 5 stars. The reviewer used it at a 1:1500 dilution for Western Blot on cancer cell lines from various tissues. They sorted cancer stem cell subpopulations by flow cytometry and used a panel of stem cell–related antibodies for detection. ABCG1, one of the candidate stem cell markers, produced clear bands, indicating reliable performance in their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Shiford (Verified Customer) (09-24-2023) | We sorted cancer stem cell subpopulations from multiple cell lines by flow cytometry and used a cluster of antibodies related to stem cell characteristics to detect. ABCG1 is one of the possible stem cell markers, with clear bands.
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