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ABCG1 Antibody 11 Publications

Rabbit Polyclonal| Catalog number: 13578-1-AP

Featured Product KD/KO validated

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Con: 36 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

ABCG1 Antibody WB HeLa cells 13578-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 13578-1-AP (ABCG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

ABCG1 Antibody IHC human cervical cancer tissue 13578-1-AP

Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13578-1-AP(ABCG1 antibody) at dilution of 1:100 (under 10x lens)

ABCG1 Antibody IHC human cervical cancer tissue 13578-1-AP

Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13578-1-AP(ABCG1 antibody) at dilution of 1:100 (under 40x lens)

ABCG1 Antibody IP mouse brain tissue 13578-1-AP

IP Result of anti-ABCG1 (IP:13578-1-AP, 4ug; Detection:13578-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
HeLa cells, RAW 264.7 cells, HepG2 cells
Positive IP detected in:
mouse brain tissue
Positive IHC detected in:
human cervical cancer tissue, human tonsillitis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ABCG1 fusion protein Ag4479
Full name:
ATP-binding cassette, sub-family G (WHITE), member 1
Calculated molecular weight:
678aa,76 kDa
Observed molecular weight:
90-100 kDa
GenBank accession number:
BC029158
Gene ID (NCBI):
9619
Gene symbol
ABCG1
Synonyms
ABC8, ABCG1, White protein homolog, WHITE1, WHT1

Background

ABCG1 is a cholesterol and phospholipid transporter expressed in lung, brain, spleen, and macrophages. In liver, ABCG1 is expressed mainly in Kupffer cells. The monomer of ABCG1 is around 60-65 kDa, while multiple forms of ABCG1 can be observed on SDS-PAGE, including 130 kDa (dimer), 90-95 kDa, 110 kDa. It was not clear whether the glycosylation or splicing accounts for the differences in mass. (PMID: 16702602)


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