ABCG1 Polyclonal antibody

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  WB analysis of RAW 264.7 using 13578-1-AP

  WB result of ABCG1 antibody (13578-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABCG1 transfected RAW 264.7 cells.

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  WB analysis using 13578-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13578-1-AP (ABCG1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13578-1-AP

  IP result of anti-ABCG1 (IP:13578-1-AP, 4ug; Detection:13578-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse placenta using 13578-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 13578-1-AP (ABCG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse placenta using 13578-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 13578-1-AP (ABCG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 13578-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13578-1-AP (ABCG1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervical cancer using 13578-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13578-1-AP (ABCG1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human tonsillitis using 13578-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13578-1-AP (ABCG1 Antibody) at dilution of 1:50.

• Featured Product
• KD/KO Validated

ABCG1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 13578-1-AP

Publications(57)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

ABC8, ABCG1, White protein homolog, WHITE1, WHT1

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Tested Applications

• Positive WB detected in: HUVEC cells, RAW 264.7 cells, HeLa cells, HepG2 cells, mouse kidney tissue, rat kidney tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse placenta tissue, human tonsillitis tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 50 publications below
• IHC: See 7 publications below
• IF: See 10 publications below

Product Information

13578-1-AP targets ABCG1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABCG1 fusion protein Ag4479
• Full Name: ATP-binding cassette, sub-family G (WHITE), member 1
• Calculated Molecular Weight: 678 aa, 76 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC029158
• Gene Symbol: ABCG1
• Gene ID (NCBI): 9619
• RRID: AB_2220174
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P45844
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABCG1 is a cholesterol and phospholipid transporter expressed in lung, brain, spleen, and macrophages. In liver, ABCG1 is expressed mainly in Kupffer cells. The monomer of ABCG1 is around 60-65 kDa, while multiple forms of ABCG1 can be observed on SDS-PAGE, including 130 kDa (dimer), 90-95 kDa, 110 kDa. It was not clear whether the glycosylation or splicing accounts for the differences in mass. (PMID: 16702602)

Publications

Species	Application	Title
human	WB	Cancer Res; PRMT1 sustains de novo fatty acid synthesis by methylating PHGDH to drive chemoresistance in triple-negative breast cancer; Authors - Takehiro Yamamoto
mouse	WB	Autophagy; Metabolic effects of RUBCN/Rubicon deficiency in kidney proximal tubular epithelial cells.; Authors - Jun Matsuda
human	WB	Br J Pharmacol; Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy.; Authors - Chuanrui Ma
human	IF	J Pharm Anal; Targeted delivery of rosuvastatin enhances treatment of hyperhomocysteinemia-induced atherosclerosis using macrophage membrane-coated nanoparticles; Authors - Dayue Liu
mouse	WB,IF	Phytomedicine; MaiJiTong granule attenuates atherosclerosis by reducing ferroptosis via activating STAT6-mediated inhibition of DMT1 and SOCS1/p53 pathways in LDLR-/- mice; Authors - Jia Shi
mouse	IHC	Phytomedicine; Rice bran active peptide (RBAP) inhibited macrophage differentiation to foam cell and atherosclerosis in mice via regulating cholesterol efflux; Authors - Jianfei Mu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shiford (Verified Customer) (09-24-2023)

We sorted cancer stem cell subpopulations from multiple cell lines by flow cytometry and used a cluster of antibodies related to stem cell characteristics to detect. ABCG1 is one of the possible stem cell markers, with clear bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1：1500
• Cell Tissue Type: Cancer cell lines from various tissues

ABCG1 Antibody Western Blot validation (1：1500 dilution) in Cancer cell lines from various tissues (Cat no:13578-1-AP)