ABL2 Polyclonal antibody

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  WB analysis using 17693-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17693-1-AP (ABL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17693-1-AP

  IP result of anti-ABL2 (IP:17693-1-AP, 4ug; Detection:17693-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of human gliomas using 17693-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17693-1-AP (ABL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 17693-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17693-1-AP (ABL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

ABL2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 17693-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

Abelson tyrosine-protein kinase 2, Abelson-related gene protein, ABLL, ARG, EC:2.7.10.2

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

17693-1-AP targets ABL2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11860

  Product name: Recombinant human ABL2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 822-1167 aa of BC065912

  Sequence: SEESAAPSRERPKAKLLPRGATALPLRTPSGDLAITEKDPPGVGVAGVAAAPKGKEKNGGARLGMAGVPEDGEQPGWPSPAKAAPVLPTTHNHKVPVLISPTLKHTPADVQLIGTDSQGNKFKLLSEHQVTSSGDKDRPRRVKPKCAPPPPPVMRLLQHPSICSDPTEEPTALTAGQSTSETQEGGKKAALGAVPISGKAGRPVMPPPQVPLPTSSISPAKMANGTAGTKVALRKTKQAAEKISADKISKEALLECADLLSSALTEPVPNSQLVDTGHQLLDYCSGYVDCIPQTRNKFAFREAVSKLELSLQELQVSSAAAGVPGTNPVLNNLLSCVQEISDVVQR

• Full Name: v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene)
• Calculated Molecular Weight: 1167 aa, 127 kDa
• Observed Molecular Weight: 128-140 kDa
• GenBank Accession Number: BC065912
• Gene Symbol: ABL2
• Gene ID (NCBI): 27
• RRID: AB_2289025
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P42684
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABL2(Abelson tyrosine-protein kinase 2) is also named as ABLL, ARG and belongs to the protein kinase superfamily. It is a membrane-anchored nonreceptor tyrosine kinase that is a novel mediator of SEMA3F-induced RhoA inactivation and collapsing activity and it has a role in cytoskeletal rearrangements by its C-terminal F-actin- and microtubule-binding sequences. ABL2 can be polyubiquitinated and the polyubiquitination of ABL2 leads to degradation. This protein has 9 isoforms produced by alternative splicing with the molecular weight from 114 kDa to 128 kDa. The antibody is specific to the others except the 60 kDa isoform of ABL2.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	IF	Bioact Mater; High proportion strontium-doped micro-arc oxidation coatings enhance early osseointegration of titanium in osteoporosis by anti-oxidative stress pathway.; Authors - Xinkun Shen
rat	WB	Theranostics; Non-invasive photobiomodulation treatment in an Alzheimer Disease-like transgenic rat model.; Authors - Luodan Yang
human	WB	Elife; Abelson tyrosine-protein kinase 2 regulates myoblast proliferation and controls muscle fiber length.; Authors - Jennifer K Lee ; KO Validated
human	WB,IHC,CoIP	Oncogene; Phosphorylation of MICAL2 by ARG promotes head and neck cancer tumorigenesis by regulating skeletal rearrangement; Authors - Ze Zhang
human	WB	Genomics; Bone marrow mesenchymal stem cells-derived exosomal microRNA-19b-1-5p reduces proliferation and raises apoptosis of bladder cancer cells via targeting ABL2.; Authors - Dewang Fu
human	WB	Biochim Biophys Acta; Abl2 kinase phosphorylates Bi-organellar regulator MNRR1 in mitochondria, stimulating respiration.; Authors - Siddhesh Aras