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  • KD/KO Validated

ABL2 Polyclonal antibody

ABL2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17693-1-AP

Synonyms

Abelson related gene protein, ABL2, ABLL, ARG, Tyrosine protein kinase ABL2, Tyrosine protein kinase ARG



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

17693-1-AP targets ABL2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABL2 fusion protein Ag11860
Full Name v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene)
Calculated molecular weight 1167 aa, 127 kDa
Observed molecular weight 128 kDa-140 kDa
GenBank accession numberBC065912
Gene symbol ABL2
Gene ID (NCBI) 27
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ABL2(Abelson tyrosine-protein kinase 2) is also named as ABLL, ARG and belongs to the protein kinase superfamily. It is a membrane-anchored nonreceptor tyrosine kinase that is a novel mediator of SEMA3F-induced RhoA inactivation and collapsing activity and it has a role in cytoskeletal rearrangements by its C-terminal F-actin- and microtubule-binding sequences. ABL2 can be polyubiquitinated and the polyubiquitination of ABL2 leads to degradation. This protein has 9 isoforms produced by alternative splicing with the molecular weight from 114 kDa to 128 kDa. The antibody is specific to the others except the 60 kDa isoform of ABL2.

Protocols

Product Specific Protocols
WB protocol for ABL2 antibody 17693-1-APDownload protocol
IHC protocol for ABL2 antibody 17693-1-APDownload protocol
IF protocol for ABL2 antibody 17693-1-APDownload protocol
IP protocol for ABL2 antibody 17693-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Rep

Timely Inhibitory Circuit Formation Controlled by Abl1 Regulates Innate Olfactory Behaviors in Mouse.

Authors - Jae Yeon Kim
humanWB

Kaohsiung J Med Sci

miR-143 inhibits renal cell carcinoma cells metastatic potential by suppressing ABL2.

Authors - Bin Xu
humanWB

Biochim Biophys Acta

Abl2 kinase phosphorylates Bi-organellar regulator MNRR1 in mitochondria, stimulating respiration.

Authors - Siddhesh Aras
humanWB

Elife

Abelson tyrosine-protein kinase 2 regulates myoblast proliferation and controls muscle fiber length.

Authors - Jennifer K Lee
  • KO Validated