|Positive WB detected in||HeLa cells, Jurkat cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human liver cancer tissue, human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
17693-1-AP targets ABL2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ABL2 fusion protein Ag11860|
|Full Name||v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene)|
|Calculated molecular weight||1167 aa, 127 kDa|
|Observed molecular weight||128 kDa-140 kDa|
|GenBank accession number||BC065912|
|Gene ID (NCBI)||27|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ABL2(Abelson tyrosine-protein kinase 2) is also named as ABLL, ARG and belongs to the protein kinase superfamily. It is a membrane-anchored nonreceptor tyrosine kinase that is a novel mediator of SEMA3F-induced RhoA inactivation and collapsing activity and it has a role in cytoskeletal rearrangements by its C-terminal F-actin- and microtubule-binding sequences. ABL2 can be polyubiquitinated and the polyubiquitination of ABL2 leads to degradation. This protein has 9 isoforms produced by alternative splicing with the molecular weight from 114 kDa to 128 kDa. The antibody is specific to the others except the 60 kDa isoform of ABL2.
|Product Specific Protocols|
|WB protocol for ABL2 antibody 17693-1-AP||Download protocol|
|IHC protocol for ABL2 antibody 17693-1-AP||Download protocol|
|IF protocol for ABL2 antibody 17693-1-AP||Download protocol|
|IP protocol for ABL2 antibody 17693-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Timely Inhibitory Circuit Formation Controlled by Abl1 Regulates Innate Olfactory Behaviors in Mouse.
Kaohsiung J Med Sci
miR-143 inhibits renal cell carcinoma cells metastatic potential by suppressing ABL2.
Biochim Biophys Acta
Abl2 kinase phosphorylates Bi-organellar regulator MNRR1 in mitochondria, stimulating respiration.
Abelson tyrosine-protein kinase 2 regulates myoblast proliferation and controls muscle fiber length.