• Featured Product
  • KD/KO Validated

ABLIM1 Polyclonal antibody

ABLIM1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15129-1-AP

Synonyms

ABLIM, abLIM 1, ABLIM1, actin binding LIM protein 1, KIAA0059, LIMAB1, LIMATIN



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, NCI-H1299 cells, Y79 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse skeletal muscle tissue, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15129-1-AP targets ABLIM1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABLIM1 fusion protein Ag7258
Full Name actin binding LIM protein 1
Calculated molecular weight 88 kDa
Observed molecular weight 48-52 kDa, 105-110 kDa
GenBank accession numberBC002448
Gene symbol ABLIM1
Gene ID (NCBI) 3983
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ABLIM1 (actin-binding LIM protein 1) is a member of the LIM-domain protein family, mediating interactions between actin filaments and cytoplasmic targets. ABLIM1 has been reported as a cell cortex protein specifically in non-erythrocytes, and is critical for the formation of dense interwoven cortical actin meshwork. Several isoforms of ABLIM1 exist due to the alternative splicing. This antibody recognized 110 kDa and 48 kDa forms of ABLIM1. The phosphorylation or binding with SUMO2 may result in higher MW (predicted MW 88 kDa).

Protocols

Product Specific Protocols
WB protocol for ABLIM1 antibody 15129-1-APDownload protocol
IHC protocol for ABLIM1 antibody 15129-1-APDownload protocol
IF protocol for ABLIM1 antibody 15129-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.

Authors - Cao Jingli J
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joleen (Verified Customer) (05-29-2019)

ABLIM should be around 87kD, but the 2 prominent bands do not come near that molecular weight. The top band is just above the 100kD molecular weight marker and the lower band is below the 75kD molecular weight marker. (BioRad Precision Plus Protein Standard used).

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: MDCK GII
ABLIM1 Antibody Western Blot, validation (1:1000 dilution) in MDCK GII (Cat no:15129-1-AP)