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  • KD/KO Validated

ABLIM1 Polyclonal antibody

ABLIM1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 15129-1-AP


ABLIM, abLIM 1, ABLIM1, actin binding LIM protein 1, KIAA0059, LIMAB1, LIMATIN

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, NCI-H1299 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse skeletal muscle tissue, HEK-293 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15129-1-AP targets ABLIM1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABLIM1 fusion protein Ag7258
Full Name actin binding LIM protein 1
Calculated Molecular Weight 88 kDa
Observed Molecular Weight 48-52 kDa, 105-110 kDa
GenBank Accession NumberBC002448
Gene Symbol ABLIM1
Gene ID (NCBI) 3983
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABLIM1 (actin-binding LIM protein 1) is a member of the LIM-domain protein family, mediating interactions between actin filaments and cytoplasmic targets. ABLIM1 has been reported as a cell cortex protein specifically in non-erythrocytes, and is critical for the formation of dense interwoven cortical actin meshwork. Several isoforms of ABLIM1 exist due to the alternative splicing. This antibody recognized 110 kDa and 48 kDa forms of ABLIM1. The phosphorylation or binding with SUMO2 may result in higher MW (predicted MW 88 kDa).


Product Specific Protocols
WB protocol for ABLIM1 antibody 15129-1-APDownload protocol
IHC protocol for ABLIM1 antibody 15129-1-APDownload protocol
IF protocol for ABLIM1 antibody 15129-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Cell Biol

miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.

Authors - Cao Jingli J
  • KD Validated

J Cell Physiol

Actin binding LIM 1 (abLIM1) negatively controls osteoclastogenesis by regulating cell migration and fusion.

Authors - Haruna Narahara
  • KD Validated

Int J Biol Sci

Rictor promotes cell migration and actin polymerization through regulating ABLIM1 phosphorylation in Hepatocellular Carcinoma.

Authors - Xin Dong
  • KD Validated

Mol Biol Cell

Spatial proximity of proteins surrounding zyxin under force-bearing conditions.

Authors - Joleen S Cheah


Antigenic responses are hallmarks of fibrotic interstitial lung diseases independent of underlying etiologies

Authors - Young Me Yoon

Circ Res

GSK-3β Localizes to the Cardiac Z-Disc to Maintain Length Dependent Activation

Authors - Marisa J Stachowski-Doll


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Joleen (Verified Customer) (05-29-2019)

ABLIM should be around 87kD, but the 2 prominent bands do not come near that molecular weight. The top band is just above the 100kD molecular weight marker and the lower band is below the 75kD molecular weight marker. (BioRad Precision Plus Protein Standard used).

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: MDCK GII
ABLIM1 Antibody Western Blot, validation (1:1000 dilution) in MDCK GII (Cat no:15129-1-AP)