ACADL-Specific Polyclonal antibody

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  WB analysis using 17526-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17526-1-AP (ACADL-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17526-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17526-1-AP (ACADL-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 17526-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17526-1-AP (ACADL-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 17526-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17526-1-AP (ACADL-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 17526-1-AP

  IP result of anti-ACADL-Specific (IP:17526-1-AP, 4ug; Detection:17526-1-AP 1:10000) with mouse kidney tissue lysate 2160 ug.

• Featured Product
• KD/KO Validated

ACADL-Specific Polyclonal Antibody for WB, IP, ELISA

Cat No. 17526-1-AP

Publications(56)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

ACADL, Long-chain specific acyl-CoA dehydrogenase, mitochondrial, LCAD, EC:1.3.8.8, ACAD4

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse heart tissue, mouse liver tissue, mouse lung tissue
• Positive IP detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 51 publications below
• IHC: See 5 publications below
• IF: See 2 publications below
• IP: See 4 publications below

Product Information

17526-1-AP targets ACADL-Specific in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, sheep, ewes
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: acyl-Coenzyme A dehydrogenase, long chain
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: NM_001608
• Gene Symbol: ACADL
• Gene ID (NCBI): 33
• RRID: AB_2219661
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P28330
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACADL(Long-chain specific acyl-CoA dehydrogenase, mitochondrial) is also named as LCAD and belongs to the acyl-CoA dehydrogenase family.It catalyzes the first reaction of the mitochondrial β-oxidation of fatty acids and is synthesized in the cytosol as a precursor that is larger than its mature from. The size difference between the precursor and mature rat LCAD has been estimated to be 3 kDa on the basis of SDS-PAGE(PMID:1774065). Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD).This antibody is specific to ACADL.

Publications

Species	Application	Title
mouse	WB	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao
mouse	WB	Circ Res; NAD+ Repletion Reverses Heart Failure With Preserved Ejection Fraction.; Authors - Dan Tong
human	WB,IF	Int J Biol Sci; Bulk and single-cell transcriptome profiling reveal extracellular matrix mechanical regulation of lipid metabolism reprograming through YAP/TEAD4/ACADL axis in hepatocellular carcinoma; Authors - Jingwei Cai
mouse	WB	Diabetes; Pivotal Role of O-GlcNAc Modification in Cold-Induced Thermogenesis by Brown Adipose Tissue Through Mitochondrial Biogenesis.; Authors - Natsuko Ohashi
rat	WB	Phytomedicine; Shen-Shuai-II-Recipe inhibits tubular inflammation by PPARα-mediated fatty acid oxidation to attenuate fibroblast activation in fibrotic kidneys; Authors - Meng Wang
mouse, human	WB, IHC	Cell Rep; HIF-1-Mediated Suppression of Acyl-CoA Dehydrogenases and Fatty Acid Oxidation Is Critical for Cancer Progression.; Authors - De Huang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rebeca (Verified Customer) (03-11-2024)

I had a great experience ordering this product and it arrived within a few days.

• Applications: Western Blot

FH

Wu (Verified Customer) (04-10-2021)

LCAD antibody worked good in IF assay at 1:50 dilution.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Liver

ACADL-Specific Antibody Immunofluorescence validation (1:50 dilution) in Liver (Cat no:17526-1-AP)

FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain