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ACADL-Specific Antibody 11 Publications

Rabbit Polyclonal| Catalog number: 17526-1-AP

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Tested Applications

Applications:
WB, IHC, ELISA
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ACADL-Specific Antibody WB mouse kidney tissue 17526-1-AP

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17526-1-AP (ACADL-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

ACADL-Specific Antibody IHC human colon tissue 17526-1-AP

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17526-1-AP (ACADL-Specific antibody) at dilution of 1:1000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ACADL-Specific Antibody IHC human colon tissue 17526-1-AP

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17526-1-AP (ACADL-Specific antibody) at dilution of 1:1000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse kidney tissue, mouse lung tissue, mouse heart tissue
Positive IHC detected in:
human colon tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:1000-1:4000
IHC : 1:500-1:2000
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
acyl-Coenzyme A dehydrogenase, long chain
Calculated molecular weight:
48 kDa
Observed molecular weight:
48 kDa
GenBank accession number:
NM_001608
Gene ID (NCBI):
33
Gene symbol
ACADL
Synonyms
ACAD4, ACADL, LCAD

Background

ACADL(Long-chain specific acyl-CoA dehydrogenase, mitochondrial) is also named as LCAD and belongs to the acyl-CoA dehydrogenase family.It catalyzes the first reaction of the mitochondrial β-oxidation of fatty acids and is synthesized in the cytosol as a precursor that is larger than its mature from. The size difference between the precursor and mature rat LCAD has been estimated to be 3 kDa on the basis of SDS-PAGE(PMID:1774065).Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD).This antibody is specific to ACADL.


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