|Positive WB detected in||mouse kidney tissue, mouse lung tissue, mouse heart tissue|
|Positive IHC detected in||human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
17526-1-AP targets ACADL-Specific in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, pig|
|Host / Isotype||Rabbit / IgG|
|Full Name||acyl-Coenzyme A dehydrogenase, long chain|
|Calculated molecular weight||48 kDa|
|Observed molecular weight||48 kDa|
|GenBank accession number||NM_001608|
|Gene ID (NCBI)||33|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ACADL(Long-chain specific acyl-CoA dehydrogenase, mitochondrial) is also named as LCAD and belongs to the acyl-CoA dehydrogenase family.It catalyzes the first reaction of the mitochondrial β-oxidation of fatty acids and is synthesized in the cytosol as a precursor that is larger than its mature from. The size difference between the precursor and mature rat LCAD has been estimated to be 3 kDa on the basis of SDS-PAGE(PMID:1774065).Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD).This antibody is specific to ACADL.
Berberine alleviates nonalcoholic fatty liver induced by a high-fat diet in mice by activating SIRT3.
Front Cell Dev Biol
Reprogramming of Lipid Metabolism as a New Driving Force Behind Tauroursodeoxycholic Acid-Induced Neural Stem Cell Proliferation.
J Mol Med (Berl)
Exogenous H2S switches cardiac energy substrate metabolism by regulating SIRT3 expression in db/db mice.
J Pharm Pharmacol
Enzogenol improves diabetes-related metabolic change in C57BL/KsJ-db/db mice, a model of type 2 diabetes mellitus.
J Physiol Biochem
GCN5L1 controls renal lipotoxicity through regulating acetylation of fatty acid oxidation enzymes.
Am J Physiol Heart Circ Physiol
Acetylation of mitochondrial proteins by GCN5L1 promotes enhanced fatty acid oxidation in the heart.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tanusree (Verified Customer) (12-18-2019)
Product worked well in WB at 1:500 dilution