• Featured Product
  • KD/KO Validated

ACADL-Specific Polyclonal antibody

ACADL-Specific Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17526-1-AP

Synonyms

ACAD4, ACADL, LCAD



Tested Applications

Positive WB detected inmouse kidney tissue, mouse lung tissue, mouse heart tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

17526-1-AP targets ACADL-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name acyl-Coenzyme A dehydrogenase, long chain
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberNM_001608
Gene symbol ACADL
Gene ID (NCBI) 33
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACADL(Long-chain specific acyl-CoA dehydrogenase, mitochondrial) is also named as LCAD and belongs to the acyl-CoA dehydrogenase family.It catalyzes the first reaction of the mitochondrial β-oxidation of fatty acids and is synthesized in the cytosol as a precursor that is larger than its mature from. The size difference between the precursor and mature rat LCAD has been estimated to be 3 kDa on the basis of SDS-PAGE(PMID:1774065).Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD).This antibody is specific to ACADL.

Protocols

Product Specific Protocols
WB protocol for ACADL-Specific antibody 17526-1-APDownload protocol
IHC protocol for ACADL-Specific antibody 17526-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

FASEB J

Berberine alleviates nonalcoholic fatty liver induced by a high-fat diet in mice by activating SIRT3.

Authors - Xi Xu
humanIHC

Front Cell Dev Biol

Reprogramming of Lipid Metabolism as a New Driving Force Behind Tauroursodeoxycholic Acid-Induced Neural Stem Cell Proliferation.

Authors - Marta B Fernandes
mouseWB

J Mol Med (Berl)

Exogenous H2S switches cardiac energy substrate metabolism by regulating SIRT3 expression in db/db mice.

Authors - Yu Sun
mouseWB

J Pharm Pharmacol

Enzogenol improves diabetes-related metabolic change in C57BL/KsJ-db/db mice, a model of type 2 diabetes mellitus.

Authors - Chae-Young Bang
WB

J Physiol Biochem

GCN5L1 controls renal lipotoxicity through regulating acetylation of fatty acid oxidation enzymes.

Authors - Tingting Lv
mouseWB

Am J Physiol Heart Circ Physiol

Acetylation of mitochondrial proteins by GCN5L1 promotes enhanced fatty acid oxidation in the heart.

Authors - Dharendra Thapa

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain