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ACADL-Specific Antibody 13 Publications

Rabbit Polyclonal| Catalog number: 17526-1-AP

Featured Product KD/KO validated

1 customer review

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Con: 40 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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ACADL-Specific Antibody WB mouse kidney tissue 17526-1-AP

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17526-1-AP (ACADL-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

ACADL-Specific Antibody IHC human colon tissue 17526-1-AP

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17526-1-AP (ACADL-Specific antibody) at dilution of 1:1000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ACADL-Specific Antibody IHC human colon tissue 17526-1-AP

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17526-1-AP (ACADL-Specific antibody) at dilution of 1:1000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse kidney tissue, mouse lung tissue, mouse heart tissue
Positive IHC detected in:
human colon tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:1000-1:4000
IHC : 1:500-1:2000
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
acyl-Coenzyme A dehydrogenase, long chain
Calculated molecular weight:
48 kDa
Observed molecular weight:
48 kDa
GenBank accession number:
NM_001608
Gene ID (NCBI):
33
Gene symbol
ACADL
Synonyms
ACAD4, ACADL, LCAD

Background

ACADL(Long-chain specific acyl-CoA dehydrogenase, mitochondrial) is also named as LCAD and belongs to the acyl-CoA dehydrogenase family.It catalyzes the first reaction of the mitochondrial β-oxidation of fatty acids and is synthesized in the cytosol as a precursor that is larger than its mature from. The size difference between the precursor and mature rat LCAD has been estimated to be 3 kDa on the basis of SDS-PAGE(PMID:1774065).Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD).This antibody is specific to ACADL.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

ACADL-Specific Rabbit Polyclonal antibody

Tanusree (Verified Customer), 12-18-2019
Applications
Western Blot,
Primary Antibody Dilution
1:500
No image submitted with review

No image submitted with review

Cell/Tissue Type
Mouse brain
Lot Number
00051417
Product worked well in WB at 1:500 dilution

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