|Positive WB detected in||mouse liver tissue, HEK-293 cells, mouse heart tissue, HepG2 cells, rat heart tissue, K-562 cells|
|Positive IHC detected in||human liver tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
55210-1-AP targets ACADM in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain|
|Calculated molecular weight||47 kDa|
|Observed molecular weight||45 kDa|
|GenBank accession number||NM_000016|
|Gene ID (NCBI)||34|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
chao (Verified Customer) (01-30-2020)
Single strong, clear band for heart lysates