ACADM Antibody

• Featured Product
• KD/KO Validated

ACADM Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Publications

17

Reviews (1)

Cat no : 55210-1-AP

Synonyms

ACAD1, ACADM, MCAD, MCADH

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Validation Data Gallery

• 

  WB analysis of mouse liver tissue using 55210-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

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Tested Applications

• Positive WB detected in: mouse liver tissue, HEK-293 cells, mouse heart tissue, HepG2 cells, rat heart tissue, K-562 cells
• Positive IHC detected in: human liver tissue, human heart tissue; ; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 3 publications below

Product Information

55210-1-AP targets ACADM in IHC, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Reactivity: human, mouse, rat
• Cited Species: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
• Calculated molecular weight: 47 kDa
• Observed molecular weight: 45 kDa
• GenBank accession number: NM_000016
• Gene symbol: ACADM
• Gene ID (NCBI): 34
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Background Information

ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.

Publications

Species	Application	Title
human	WB	Sci Rep; New Self-assembled Supramolecular Bowls as Potent Anticancer Agents for Human Hepatocellular Carcinoma.; Authors - Hae Seong Song
mouse	WB	Am J Physiol Gastrointest Liver Physiol; Inhibition of soluble epoxide hydrolase ameliorates hyperhomocysteinemia-induced hepatic steatosis by enhancing β-oxidation of fatty acid in mice.; Authors - Liu Yao
human	WB	Cell Death Dis; The contrary intracellular and extracellular functions of PEDF in HCC development.; Authors - Cen Li
human	WB	Biochim Biophys Acta; HDAC4 mediates IFN-γ induced disruption of energy expenditure-related gene expression by repressing SIRT1 transcription in skeletal muscle cells.; Authors - Mingming Fang
rat	WB	Food Funct; Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.; Authors - Deshi Dong
mouse	WB	Sci Rep; Potent effects of dioscin against obesity in mice.; Authors - Min Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

chao chen (01-30-2020)

Single strong, clear band for heart lysates

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart

No image submitted with review