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ACADM Antibody

ACADM Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 55210-1-AP

Synonyms

ACAD1, ACADM, MCAD, MCADH



Tested Applications

Positive WB detected inmouse liver tissue, HEK-293 cells, mouse heart tissue, HepG2 cells, rat heart tissue, K-562 cells
Positive IHC detected inhuman liver tissue, human heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

55210-1-AP targets ACADM in IHC, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
Calculated molecular weight 47 kDa
Observed molecular weight 45 kDa
GenBank accession number NM_000016
Gene symbol ACADM
Gene ID (NCBI) 34
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.

Protocols

Product Specific Protocols
WB protocol for ACADM antibody 55210-1-APDownload protocol
IHC protocol for ACADM antibody 55210-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

New Self-assembled Supramolecular Bowls as Potent Anticancer Agents for Human Hepatocellular Carcinoma.

Authors - Hae Seong Song
mouseWB

Am J Physiol Gastrointest Liver Physiol

Inhibition of soluble epoxide hydrolase ameliorates hyperhomocysteinemia-induced hepatic steatosis by enhancing β-oxidation of fatty acid in mice.

Authors - Liu Yao
humanWB

Cell Death Dis

The contrary intracellular and extracellular functions of PEDF in HCC development.

Authors - Cen Li
humanWB

Biochim Biophys Acta

HDAC4 mediates IFN-γ induced disruption of energy expenditure-related gene expression by repressing SIRT1 transcription in skeletal muscle cells.

Authors - Mingming Fang
ratWB

Food Funct

Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.

Authors - Deshi Dong
mouseWB

Sci Rep

Potent effects of dioscin against obesity in mice.

Authors - Min Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

chao chen (01-30-2020)

Single strong, clear band for heart lysates

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart
No image submitted with review