ACADM Polyclonal antibody

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  WB analysis using 55210-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55210-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 55210-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 55210-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 55210-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 55210-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55210-1-AP (ACADM antibody) at dilution of 1:150 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 55210-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55210-1-AP (ACADM antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 55210-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55210-1-AP (ACADM antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 55210-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55210-1-AP (ACADM antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

ACADM Polyclonal Antibody for WB, IHC, ELISA

Cat No. 55210-1-AP

Publications(62)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

ACAD, ACAD 1, ACAD1, EC:1.3.8.7, MCAD

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Tested Applications

• Positive WB detected in: HEK-293 cells, K-562 cells, HepG2 cells, rat heart tissue, MDA-MB-453s cells, mouse liver tissue, rat liver tissue, mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: human liver tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 58 publications below
• IHC: See 8 publications below
• IP: See 1 publications below

Product Information

55210-1-AP targets ACADM in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: NM_000016
• Gene Symbol: ACADM
• Gene ID (NCBI): 34
• RRID: AB_10837361
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P11310
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao
mouse	IHC	Nat Cell Biol; BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma.; Authors - Jin-Tao Li
mouse	WB	Circ Res; NAD+ Repletion Reverses Heart Failure With Preserved Ejection Fraction.; Authors - Dan Tong
mouse	WB	Redox Biol; Increased fatty acid metabolism attenuates cardiac resistance to β-adrenoceptor activation via mitochondrial reactive oxygen species: A potential mechanism of hypoglycemia-induced myocardial injury in diabetes.; Authors - Lishan Huang
mouse	WB	Cell Biosci; Hif-2α regulates lipid metabolism in alcoholic fatty liver disease through mitophagy; Authors - Mei-Fei Wu
human	WB	EMBO Rep; Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.; Authors - Yang Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yasuyo (Verified Customer) (07-22-2021)

Works good consistently.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:150
• Cell Tissue Type: liver

ACADM Antibody Immunohistochemistry validation (1:150 dilution) in liver (Cat no:55210-1-AP)

FH

chao (Verified Customer) (01-30-2020)

Single strong, clear band for heart lysates

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart