Name: ACADM Rabbit Polyclonal antibody
Brand: Proteintech

Tested Applications

Applications:

WB, IHC, ELISA
Application not tested? Please see our Guarantee

ACADM Antibody WB mouse liver tissue 55210-1-AP

mouse liver tissue were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

Species specificity:

human, mouse, rat

Positive WB detected in:

mouse liver tissue, HEK-293 cells, mouse heart tissue, HepG2 cells, rat heart tissue, K-562 cells

Positive IHC detected in:

human liver tissue, human heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution:

WB : 1:1000-1:4000

IHC : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

Peptide

Full name:

acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain

Calculated molecular weight:

47 kDa

Observed molecular weight:

45 kDa

GenBank accession number:

NM_000016

Gene ID (NCBI):

Gene symbol

ACADM

Synonyms

ACAD1, ACADM, MCAD, MCADH

Background

ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.

Showing 1- [6] of [10] Publications

Author:		De Huang
Pubmed ID:		25242319
Journal:		Cell Rep
Title:		HIF-1-Mediated Suppression of Acyl-CoA Dehydrogenases and Fatty Acid Oxidation Is Critical for Cancer Progression.
Application:		WB, IHC
KD validated
Species:		mouse, human

Author:		Deshi Dong
Pubmed ID:		25310017
Journal:		Food Funct
Title:		Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.
Application:		WB

Species:		rat

Author:		Min Liu
Pubmed ID:		25609476
Journal:		Sci Rep
Title:		Potent effects of dioscin against obesity in mice.
Application:		WB

Species:		mouse

Author:		Mingming Fang
Pubmed ID:		26619800
Journal:		Biochim Biophys Acta
Title:		HDAC4 mediates IFN-γ induced disruption of energy expenditure-related gene expression by repressing SIRT1 transcription in skeletal muscle cells.
Application:		WB

Species:		human

Author:		Guolin Li
Pubmed ID:		29146411
Journal:		Mol Metab
Title:		Metabolic adaptation to intermittent fasting is independent of peroxisome proliferator-activated receptor alpha.
Application:		WB

Species:		mouse

Author:		Xi Yang
Pubmed ID:		29350678
Journal:		Exp Mol Med
Title:		PIK3R3 regulates PPARα expression to stimulate fatty acid β-oxidation and decrease hepatosteatosis.
Application:		WB,IHC

Species:		mouse

WB Figures

ACADM Antibody WB HEK-293 cells 55210-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 55210-1-AP(ACADM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

ACADM Antibody WB HepG2 cells 55210-1-AP

HepG2 cells were subjected to SDS PAGE followed by western blot with 55210-1-AP(ACADM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

ACADM Antibody WB K-562 cells 55210-1-AP

K-562 cells were subjected to SDS PAGE followed by western blot with 55210-1-AP(ACADM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

ACADM Antibody WB mouse heart tissue 55210-1-AP

mouse heart tissue were subjected to SDS PAGE followed by western blot with 55210-1-AP(ACADM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse liver tissue were subjected to SDS PAGE followed by western blot with 55210-1-AP(ACADM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse liver tissue were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

ACADM Antibody WB rat heart tissue 55210-1-AP

rat heart tissue were subjected to SDS PAGE followed by western blot with 55210-1-AP( ACADM Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

ACADM Antibody 10 Publications

Rabbit Polyclonal| Catalog number: 55210-1-AP

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background

Showing 1- [6] of [10] Publications

WB Figures

ACADM Antibody 10 Publications

Rabbit Polyclonal| Catalog number: 55210-1-AP

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Special offer: Add control/tag antibody for $99

Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background