Validation Data Gallery
|Positive WB detected in
|mouse kidney tissue, COS-7 cells, mouse liver tissue, rat kidney tissue, rat liver tissue
|Positive IP detected in
|Positive IHC detected in
|human kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:1000-1:4000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:20-1:200
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
The immunogen of 16623-1-AP is ACADS Fusion Protein expressed in E. coli.
|human, mouse, rat, monkey
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|ACADS fusion protein Ag9922
|acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain
|Calculated molecular weight
|412 aa, 44 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
ACADS is also named as SCAD(Short-chain specific acyl-CoA dehydrogenase, mitochondrial) and belongs to the acyl-CoA dehydrogenase family. It is a tetrameric mitochondrial flavoprotein. It is synthesized as a 44 kDa precursor, transported into mitochondria, and proteolytically processed to its 41 kDa mature form. Acyl-CoA de- hydrogenases (ACADs) are a family of flavoproteins that are involved in theβ-oxidation of the fatty acyl-CoA derivatives.
Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.
HIF-1-Mediated Suppression of Acyl-CoA Dehydrogenases and Fatty Acid Oxidation Is Critical for Cancer Progression.
Aging (Albany NY)
ACADS acts as a potential methylation biomarker associated with the proliferation and metastasis of hepatocellular carcinomas.
Protective effects of dioscin against alcohol-induced liver injury.
Biochim Biophys Acta Mol Cell Biol Lipids
Dietary iron overload mitigates atherosclerosis in high-fat diet-fed apolipoprotein E knockout mice: Role of dysregulated hepatic fatty acid metabolism.
Correction: Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.