|Positive WB detected in||human brain tissue, COS-7 cells, HEK-293 cells, HepG2 cells, mouse kidney tissue, mouse liver tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human kidney tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 16623-1-AP is ACADS Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat, monkey|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ACADS fusion protein Ag9922|
|Full Name||acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain|
|Calculated molecular weight||412 aa, 44 kDa|
|Observed molecular weight||42 kDa|
|GenBank accession number||BC025963|
|Gene ID (NCBI)||35|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ACADS is also named as SCAD(Short-chain specific acyl-CoA dehydrogenase, mitochondrial) and belongs to the acyl-CoA dehydrogenase family. It is a tetrameric mitochondrial flavoprotein. It is synthesized as a 44 kDa precursor, transported into mitochondria, and proteolytically processed to its 41 kDa mature form. Acyl-CoA de- hydrogenases (ACADs) are a family of flavoproteins that are involved in theβ-oxidation of the fatty acyl-CoA derivatives.
Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.
Potent effects of dioscin against obesity in mice.
Am J Physiol Heart Circ Physiol
Acetylation of mitochondrial proteins by GCN5L1 promotes enhanced fatty acid oxidation in the heart.
Correction: Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.
Biochim Biophys Acta Mol Cell Biol Lipids
Dietary iron overload mitigates atherosclerosis in high-fat diet-fed apolipoprotein E knockout mice: Role of dysregulated hepatic fatty acid metabolism.
Protective effects of dioscin against alcohol-induced liver injury.