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  • KD/KO Validated

ACADS Antibody

ACADS Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16623-1-AP

Synonyms

ACAD3, ACADS, Butyryl CoA dehydrogenase, SCAD



Tested Applications

Positive WB detected inhuman brain tissue, COS-7 cells, HEK-293 cells, HepG2 cells, mouse kidney tissue, mouse liver tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The 16623-1-AP immunogen is Fusion Protein ACADS protein expressed in E. coli.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACADS fusion protein Ag9922
Full Name acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain
Calculated molecular weight 412aa,44 kDa
Observed molecular weight 42 kDa
GenBank accession number BC025963
Gene symbol ACADS
Gene ID (NCBI) 35
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACADS is also named as SCAD(Short-chain specific acyl-CoA dehydrogenase, mitochondrial) and belongs to the acyl-CoA dehydrogenase family. It is a tetrameric mitochondrial flavoprotein. It is synthesized as a 44 kDa precursor, transported into mitochondria, and proteolytically processed to its 41 kDa mature form. Acyl-CoA de- hydrogenases (ACADs) are a family of flavoproteins that are involved in theβ-oxidation of the fatty acyl-CoA derivatives.

Protocols

Product Specific Protocols
WB protocol for ACADS antibody 16623-1-APDownload protocol
IHC protocol for ACADS antibody 16623-1-APDownload protocol
IP protocol for ACADS antibody 16623-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Food Funct

Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.

Authors - Deshi Dong
mouseWB

Sci Rep

Potent effects of dioscin against obesity in mice.

Authors - Min Liu
mouseWB

Am J Physiol Heart Circ Physiol

Acetylation of mitochondrial proteins by GCN5L1 promotes enhanced fatty acid oxidation in the heart.

Authors - Dharendra Thapa
mouse, ratWB

Arch Toxicol

Protective effects of dioscin against alcohol-induced liver injury.

Authors - Xu Tingting T
humanWB

Aging (Albany NY)

ACADS acts as a potential methylation biomarker associated with the proliferation and metastasis of hepatocellular carcinomas.

Authors - Diyu Chen
  • KD Validated
humanWB

Cell Rep

HIF-1-Mediated Suppression of Acyl-CoA Dehydrogenases and Fatty Acid Oxidation Is Critical for Cancer Progression.

Authors - De Huang