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  • KD/KO Validated

ACADS Polyclonal antibody

ACADS Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16623-1-AP

Synonyms

ACAD3, ACADS, Butyryl CoA dehydrogenase, SCAD



Tested Applications

Positive WB detected inmouse kidney tissue, COS-7 cells, mouse liver tissue, rat kidney tissue, rat liver tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 16623-1-AP is ACADS Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACADS fusion protein Ag9922
Full Name acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain
Calculated molecular weight 412 aa, 44 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC025963
Gene symbol ACADS
Gene ID (NCBI) 35
RRIDAB_10666165
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACADS is also named as SCAD(Short-chain specific acyl-CoA dehydrogenase, mitochondrial) and belongs to the acyl-CoA dehydrogenase family. It is a tetrameric mitochondrial flavoprotein. It is synthesized as a 44 kDa precursor, transported into mitochondria, and proteolytically processed to its 41 kDa mature form. Acyl-CoA de- hydrogenases (ACADs) are a family of flavoproteins that are involved in theβ-oxidation of the fatty acyl-CoA derivatives.

Protocols

Product Specific Protocols
WB protocol for ACADS antibody 16623-1-APDownload protocol
IHC protocol for ACADS antibody 16623-1-APDownload protocol
IP protocol for ACADS antibody 16623-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO Rep

Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.

Authors - Yang Zhang
humanWB

Cell Rep

HIF-1-Mediated Suppression of Acyl-CoA Dehydrogenases and Fatty Acid Oxidation Is Critical for Cancer Progression.

Authors - De Huang
humanWB

Aging (Albany NY)

ACADS acts as a potential methylation biomarker associated with the proliferation and metastasis of hepatocellular carcinomas.

Authors - Diyu Chen
  • KD Validated
mouse, ratWB

Arch Toxicol

Protective effects of dioscin against alcohol-induced liver injury.

Authors - Xu Tingting T
mouseWB

Biochim Biophys Acta Mol Cell Biol Lipids

Dietary iron overload mitigates atherosclerosis in high-fat diet-fed apolipoprotein E knockout mice: Role of dysregulated hepatic fatty acid metabolism.

Authors - Lin Xiao
ratWB

Food Funct

Correction: Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.

Authors - Deshi Dong