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ACADVL Polyclonal antibody

ACADVL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14527-1-AP



Tested Applications

Positive WB detected inmouse liver tissue, L02 cells, HeLa cells, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14527-1-AP targets ACADVL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACADVL fusion protein Ag6016
Full Name acyl-Coenzyme A dehydrogenase, very long chain
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 66-73 kDa
GenBank Accession NumberBC012912
Gene Symbol ACADVL
Gene ID (NCBI) 37
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Very long-chain acyl-CoA dehydrogenase (VLCAD) is one of four flavoproteins which catalyze the initial step of the mitochondrial b-oxidation spiral. It belongs to the acyl-CoA dehydrogenase family and is a homodimer of a 71-kDa polypeptide which contains 2 mol of FAD/mol of enzyme. The molecular mass of the nondenatured trypsinized VLCAD is 98 kDa, by gel filtration chromatography, indicating that it is a homodimer of the 48 kDa(tryptic digest) polypeptide(PMID:9461620). Defects in ACADVL are the cause of acyl-CoA dehydrogenase very long chain deficiency (ACADVLD). It has 2 isoforms(70 kDa and 68 kDa ) produced by alternative splicing and a transit peptide. This antibody is speicifc to ACADVL.


Product Specific Protocols
WB protocol for ACADVL antibody 14527-1-APDownload protocol
IHC protocol for ACADVL antibody 14527-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Oxid Med Cell Longev

Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.

Authors - Wei Wang

Cell Rep

HIF-1-Mediated Suppression of Acyl-CoA Dehydrogenases and Fatty Acid Oxidation Is Critical for Cancer Progression.

Authors - De Huang

Front Immunol

Impaired Autophagy and Defective T Cell Homeostasis in Mice with T Cell-Specific Deletion of Receptor for Activated C Kinase 1.

Authors - Guihua Qiu

Biochim Biophys Acta

Succinate accumulation impairs cardiac pyruvate dehydrogenase activity through GRP91-dependent and independent signaling pathways: therapeutic effects of ginsenoside Rb1.

Authors - Jia Li

Front Oncol

Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma.

Authors - Jinghui Lu

Biochim Biophys Acta

Pharmacological inhibition of NOX4 ameliorates alcohol-induced liver injury in mice through improving oxidative stress and mitochondrial function.

Authors - Qian Sun


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Tom (Verified Customer) (09-28-2020)

HEK293T lysate (10ug) ran on 4-20% gradient gel. Blocking in 5% BSA for 1 hour, followed by ACADVL antibody (1:1000 in block) overnight at 4 degrees. Goat anti-rabbit HRP (1:10,000) for 1 hour in block.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
ACADVL Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:14527-1-AP)