ACE Polyclonal antibody

ACE Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 24743-1-AP


ACE, ACE1, Angiotensin converting enzyme, CD143, DCP, DCP1, Dipeptidyl carboxypeptidase I, Kininase II, MVCD3

Tested Applications

Positive WB detected inmouse heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman lung tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24743-1-AP is ACE Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACE fusion protein Ag20654
Full Name angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
Calculated Molecular Weight 150 kDa
Observed Molecular Weight 170 kDa
GenBank Accession NumberBC036375
Gene Symbol ACE
Gene ID (NCBI) 1636
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACE(Angiotensin-converting enzyme) is also named as DCP, DCP1 and belongs to the peptidase M2 family. It is involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. ACE also has a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. It has 4 isoforms produced by alternative splicing. The full length protein has 15 glycosylation sites and it can show a molecular weight of 170 kDa(PMID:16804085).


Product Specific Protocols
WB protocol for ACE antibody 24743-1-APDownload protocol
IHC protocol for ACE antibody 24743-1-APDownload protocol
IP protocol for ACE antibody 24743-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Oxid Med Cell Longev

Tissue Renin-Angiotensin System (tRAS) Induce Intervertebral Disc Degeneration by Activating Oxidative Stress and Inflammatory Reaction.

Authors - Kaiqiang Sun

J Cell Mol Med

PMID: 32073745

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Diabetol Metab Syndr

Celastrol attenuates streptozotocin-induced diabetic cardiomyopathy in mice by inhibiting the ACE / Ang II / AGTR1 signaling pathway

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ACS Omega

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Non-Invasive Local Acoustic Therapy Ameliorates Diabetic Heart Fibrosis by Suppressing ACE-Mediated Oxidative Stress and Inflammation in Cardiac Fibroblasts.

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Front Endocrinol (Lausanne)

TMPRSS2 Expression and Activity Modulation by Sex-Related Hormones in Lung Calu-3 Cells: Impact on Gender-Specific SARS-CoV-2 Infection.

Authors - Donatella Treppiedi