ACE Polyclonal antibody

ACE Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC

Conjugate

Unconjugated

Cat no : 24743-1-AP

Synonyms

ACE, ACE1, Angiotensin converting enzyme, CD143, DCP, DCP1, Dipeptidyl carboxypeptidase I, Kininase II, MVCD3



Tested Applications

Positive WB detected inmouse heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman lung tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24743-1-AP is ACE Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACE fusion protein Ag20654
Full Name angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
Calculated molecular weight 150 kDa
Observed molecular weight 170 kDa
GenBank accession numberBC036375
Gene symbol ACE
Gene ID (NCBI) 1636
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACE(Angiotensin-converting enzyme) is also named as DCP, DCP1 and belongs to the peptidase M2 family. It is involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. ACE also has a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. It has 4 isoforms produced by alternative splicing. The full length protein has 15 glycosylation sites and it can show a molecular weight of 170 kDa(PMID:16804085).

Protocols

Product Specific Protocols
WB protocol for ACE antibody 24743-1-APDownload protocol
IHC protocol for ACE antibody 24743-1-APDownload protocol
IP protocol for ACE antibody 24743-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
rat

Thromb Res

RhoA-Rho associated kinase signaling leads to renin-angiotensin system imbalance and angiotensin converting enzyme 2 has a protective role in acute pulmonary embolism.

Authors - Xiaoling Xu
ratIHC

J Cell Mol Med

PMID: 32073745

Authors - Jianxia Wen
ratWB

Antioxidants (Basel)

MiR155-5p Inhibits Cell Migration and Oxidative Stress in Vascular Smooth Muscle Cells of Spontaneously Hypertensive Rats.

Authors - Nan Wu
ratWB

Biosci Rep

Jian-Pi-Yi-Shen formula enhances perindopril inhibition of chronic kidney disease progression by activation of SIRT3, modulation of mitochondrial dynamics, and antioxidant effects

Authors - Xinhui Liu

J Ethnopharmacol

Network pharmacology-based strategy for predicting active ingredients and potential targets of Yangxinshi tablet for treating heart failure.

Authors - Langdong Chen
mouse, humanWB

Front Med

H. sinensis mycelium inhibits epithelial-mesenchymal transition by inactivating the midkine pathway in pulmonary fibrosis.

Authors - Li Lu