CoraLite® Plus 488-conjugated ACF1 Monoclonal antibody

ACF1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C4F8

Cat no : CL488-67947

Synonyms

ACF1, BAZ1A, CHRAC subunit ACF1, DKFZp586E0518, FLJ14383, hACF1, hWALp1, WALp1, WCRF180

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Validation Data Gallery

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  FC experiment of HeLa using CL488-67947

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-HUMAN ACF1 (CL488-67947, Clone:1C4F8) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive FC detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67947 targets ACF1 in FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ACF1 fusion protein Ag30607
• Full Name: bromodomain adjacent to zinc finger domain, 1A
• Calculated Molecular Weight: 179 kDa
• GenBank Accession Number: NM_013448
• Gene Symbol: BAZ1A
• Gene ID (NCBI): 11177
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Related Products

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011