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Acetylcholinesterase Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 17975-1-AP

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Con: 25 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

Acetylcholinesterase Antibody WB mouse liver tissue 17975-1-AP

mouse liver tissue were subjected to SDS PAGE followed by western blot with 17975-1-AP(Acetylcholinesterase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Acetylcholinesterase Antibody IHC human liver tissue 17975-1-AP

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17975-1-AP( Acetylcholinesterase antibody at dilution of 1:50 (under 10x lens)

Acetylcholinesterase Antibody IHC human liver tissue 17975-1-AP

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17975-1-AP( Acetylcholinesterase antibody at dilution of 1:50 (under 40x lens)

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse liver tissue, NIH/3T3 cells
Positive IHC detected in:
human liver tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Acetylcholinesterase fusion protein Ag12146
Full name:
acetylcholinesterase (Yt blood group)
Calculated molecular weight:
614aa,68 kDa;640aa,71 kDa
Observed molecular weight:
66-72 kDa
GenBank accession number:
BC094752
Gene ID (NCBI):
43
Gene symbol
ACHE
Synonyms
Acetylcholinesterase, ACHE, ARACHE, N ACHE, YT

Background

The classical role of acetylcholinesterase (AChE) is to terminate cholinergic neurotransmission by rapid hydrolysis of the neurotransmitter acetylcholine (ACh) at cholinergic synapses and neuromuscular junctions(PMID:20054652). AChE activity is significantly lower in aspirates from squamous cell carcinomas and the molecular distribution of AChE in both bronchial cells and fluids consisted of amphiphilic monomers and dimers(PMID:18211261). It has 4 isoforms produced by alternative splicing.


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