Acetylcholinesterase Polyclonal antibody
Acetylcholinesterase Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IHC, ELISA
Cat no : 17975-1-AP
|Positive WB detected in||mouse liver tissue, HEK-293 cells|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17975-1-AP targets Acetylcholinesterase in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Acetylcholinesterase fusion protein Ag12146|
|Full Name||acetylcholinesterase (Yt blood group)|
|Calculated molecular weight||614aa,68 kDa; 640aa,71 kDa|
|Observed molecular weight||66-72 kDa|
|GenBank accession number||BC094752|
|Gene ID (NCBI)||43|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The classical role of acetylcholinesterase (AChE) is to terminate cholinergic neurotransmission by rapid hydrolysis of the neurotransmitter acetylcholine (ACh) at cholinergic synapses and neuromuscular junctions(PMID:20054652). AChE activity is significantly lower in aspirates from squamous cell carcinomas and the molecular distribution of AChE in both bronchial cells and fluids consisted of amphiphilic monomers and dimers(PMID:18211261). It has 4 isoforms produced by alternative splicing.
|Product Specific Protocols|
|WB protocol for Acetylcholinesterase antibody 17975-1-AP||Download protocol|
|IHC protocol for Acetylcholinesterase antibody 17975-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Near-Infrared Fluorescence Probe for Evaluating Acetylcholinesterase Activity in PC12 Cells and In Situ Tracing AChE Distribution in Zebrafish.
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Lithium Attenuates Scopolamine-Induced Memory Deficits with Inhibition of GSK-3β and Preservation of Postsynaptic Components.
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Quantification of heat shock protein 70 and acetylcholinesterase over a time course suggests environmental adaptation in a foundational molluscan species.
Aging (Albany NY)
A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Zulaikha (Verified Customer) (09-21-2022)
Consistent and reproducible results.
Dr Shane (Verified Customer) (08-17-2020)
Very good antibody and worked ok.