|Positive WB detected in||mouse liver tissue, HEK-293 cells|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17975-1-AP targets Acetylcholinesterase in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Acetylcholinesterase fusion protein Ag12146|
|Full Name||acetylcholinesterase (Yt blood group)|
|Calculated molecular weight||614aa,68 kDa; 640aa,71 kDa|
|Observed molecular weight||66-72 kDa|
|GenBank accession number||BC094752|
|Gene ID (NCBI)||43|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The classical role of acetylcholinesterase (AChE) is to terminate cholinergic neurotransmission by rapid hydrolysis of the neurotransmitter acetylcholine (ACh) at cholinergic synapses and neuromuscular junctions(PMID:20054652). AChE activity is significantly lower in aspirates from squamous cell carcinomas and the molecular distribution of AChE in both bronchial cells and fluids consisted of amphiphilic monomers and dimers(PMID:18211261). It has 4 isoforms produced by alternative splicing.
Aging (Albany NY)
A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.
Ecotoxicol Environ Saf
Quantification of heat shock protein 70 and acetylcholinesterase over a time course suggests environmental adaptation in a foundational molluscan species.
J Alzheimers Dis
Lithium Attenuates Scopolamine-Induced Memory Deficits with Inhibition of GSK-3β and Preservation of Postsynaptic Components.
Pyridostigmine Protects Against Diabetic Cardiomyopathy by Regulating Vagal Activity, Gut Microbiota, and Branched-Chain Amino Acid Catabolism in Diabetic Mice.
A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota.
Near-Infrared Fluorescence Probe for Evaluating Acetylcholinesterase Activity in PC12 Cells and In Situ Tracing AChE Distribution in Zebrafish.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Dr Shane (Verified Customer) (08-17-2020)
Very good antibody and worked ok.