Validation Data Gallery
|Positive WB detected in||mouse heart tissue, human heart tissue, human liver tissue, human skeletal muscle tissue, HeLa cells, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue|
|Positive IHC detected in||human lung cancer tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11134-1-AP targets Aconitase 2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Aconitase 2 fusion protein Ag1590|
|Full Name||aconitase 2, mitochondrial|
|Calculated molecular weight||85 kDa|
|Observed molecular weight||85 kDa|
|GenBank accession number||BC014092|
|Gene ID (NCBI)||50|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ACO2(aconitate hydratase, mitochondrial) is also named as citrate hydro-lyase and belongs to the aconitase/IPM isomerase family. It plays a key function in cellular energy production, and loss of its activity has a major impact on cellular and organismal survival. Western blot shows two bands of 83 kDa and 40 kDa. The 40 kDa fragment decreases with age and oxidative stress, whereas other fragmentation products with molecular weights between 40 and 83 kDa increased with age and MnSOD(mitochondrial manganese superoxide dismutase) deficiency(PMID:12459471). Defects in ACO2 are the cause of infantile cerebellar-retinal degeneration (ICRD).
Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.
A LON-ClpP Proteolytic Axis Degrades Complex I to Extinguish ROS Production in Depolarized Mitochondria.
Mol Cell Proteomics
A combined proteomics and metabolomics profiling of gastric cardia cancer reveals characteristic dysregulations in glucose metabolism.
Cur@SF NPs alleviate Friedreich's ataxia in a mouse model through synergistic iron chelation and antioxidation.
Iron regulatory protein deficiency compromises mitochondrial function in murine embryonic fibroblasts.
The Symbiotic Relationship between the Neural Retina and Retinal Pigment Epithelium Is Supported by Utilizing Differential Metabolic Pathways.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tanusree (Verified Customer) (12-18-2019)
Product worked well in WB at 1:500 dilution
Svitlana (Verified Customer) (03-19-2019)
SDS-PAGE: mitochondrial lysate 10 ug protein/well, 4-12% Bis-Tris.Transfer: Immobilon-FL transfer membranes O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1h at room temperature.Secondary Ab: IRDye 800CW Goat anti-Rabbit 1 h at room temperature.Lines on WB:1. BioRad Precision Plus Protein standard2. Lysate of mitochondrial fraction.