Aconitase 2 Polyclonal antibody

Aconitase 2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

20

Reviews (2)

Cat no : 11134-1-AP

Synonyms

ACO2, ACO 2, EC:4.2.1.3, Citrate hydro-lyase, Aconitase2

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Validation Data Gallery

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  WB analysis using 11134-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11134-1-AP (Aconitase 2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 11134-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11134-1-AP (Aconitase 2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11134-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11134-1-AP (Aconitase 2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 11134-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11134-1-AP (Aconitase 2 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 11134-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11134-1-AP (Aconitase 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11134-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11134-1-AP (Aconitase 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11134-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11134-1-AP (Aconitase 2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 11134-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11134-1-AP (Aconitase 2 antibody) at dilution of 1:50 (under 25x lens).

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  IF Staining of HeLa using 11134-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Aconitase 2 antibody (11134-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: mouse heart tissue, human liver tissue, human skeletal muscle tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue
• Positive IHC detected in: mouse kidney tissue, human colon tissue, human lung cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:200-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 19 publications below
• IF: See 3 publications below

Product Information

11134-1-AP targets Aconitase 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Aconitase 2 fusion protein Ag1590
• Full Name: aconitase 2, mitochondrial
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC014092
• Gene Symbol: ACO2
• Gene ID (NCBI): 50
• RRID: AB_2289288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACO2(aconitate hydratase, mitochondrial) is also named as citrate hydro-lyase and belongs to the aconitase/IPM isomerase family. It plays a key function in cellular energy production, and loss of its activity has a major impact on cellular and organismal survival. Western blot shows two bands of 83 kDa and 40 kDa. The 40 kDa fragment decreases with age and oxidative stress, whereas other fragmentation products with molecular weights between 40 and 83 kDa increased with age and MnSOD(mitochondrial manganese superoxide dismutase) deficiency(PMID:12459471). Defects in ACO2 are the cause of infantile cerebellar-retinal degeneration (ICRD).

Publications

Species	Application	Title
human	WB	Cell Metab; Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.; Authors - Yi-Ping Wang
mouse	WB	Aging Dis; Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging; Authors - Minghao Yuan
human	WB	Cell Rep; A LON-ClpP Proteolytic Axis Degrades Complex I to Extinguish ROS Production in Depolarized Mitochondria.; Authors - Kenneth Robert Pryde
human	WB	EMBO Rep; UBXN1 maintains ER proteostasis and represses UPR activation by modulating translation; Authors - Brittany A Ahlstedt
human	WB	Cell Biosci; Hinokitiol-iron complex is a ferroptosis inducer to inhibit triple-negative breast tumor growth; Authors - Hongting Zhao
human	WB	Mol Cell Proteomics; A combined proteomics and metabolomics profiling of gastric cardia cancer reveals characteristic dysregulations in glucose metabolism.; Authors - Cai Zhen Z

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

FH

Svitlana (Verified Customer) (03-19-2019)

SDS-PAGE: mitochondrial lysate 10 ug protein/well, 4-12% Bis-Tris.Transfer: Immobilon-FL transfer membranes O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1h at room temperature.Secondary Ab: IRDye 800CW Goat anti-Rabbit 1 h at room temperature.Lines on WB:1. BioRad Precision Plus Protein standard2. Lysate of mitochondrial fraction.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293t mitochondria

Aconitase 2 Antibody Western Blot, validation (1:1000 dilution) in HEK293t mitochondria (Cat no:11134-1-AP)