Validation Data Gallery
|Positive WB detected in||L02 cells, mouse cerebellum tissue, mouse liver tissue, rat liver tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human lung cancer tissue, human liver cancer tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
13989-1-AP targets ACSL1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ACSL1 fusion protein Ag5059|
|Full Name||acyl-CoA synthetase long-chain family member 1|
|Calculated molecular weight||78 kDa|
|Observed molecular weight||68 kDa, 78 kDa|
|GenBank accession number||BC050073|
|Gene ID (NCBI)||2180|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ACSL1(Long-chain-fatty-acid--CoA ligase 1) is also named as FACL1, FACL2, LACS, LACS1, LACS2 and belongs to the ATP-dependent AMP-binding enzyme family. ACSL1 is a 75 kDa protein that is associated peripherally with the plasma membrane(Brian M Wiczer, etc., 2006). ACSL1 is abundantly expressed in tissues, such as liver and brown fat, that metabolize substantial amounts of triglycerides as fuel, and as such, a deficiency in ACSL1 function could have a more profound affect in those cells, resulting in hepatosteatosis and potentially increased very low density lipoprotein production by the liver or decreased thermogenic capacity in brown adipose tissue(PMID:19429676). An anti-rat ACSL1 antibody recognized a band of the predicted 68 kDa in high-speed supernatant from rat liver and in human and murine SMCs, monocyte-derived macrophages, and murine peritoneal macrophages (PMID:17259370). It has 2 isoforms produced by alternative splicing.
|Product Specific Protocols|
|WB protocol for ACSL1 antibody 13989-1-AP||Download protocol|
|IHC protocol for ACSL1 antibody 13989-1-AP||Download protocol|
|IF protocol for ACSL1 antibody 13989-1-AP||Download protocol|
|IP protocol for ACSL1 antibody 13989-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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Thomas (Verified Customer) (10-23-2019)
Very good service, very good antibody