ACSL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ACSL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

53

Reviews (1)

Cat no : 13989-1-AP

Synonyms

EC:6.2.1.24, EC:6.2.1.15, Arachidonate--CoA ligase, Acyl-CoA synthetase 1, Acyl CoA synthetase 1

---

Validation Data Gallery

• 

  WB analysis using 13989-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13989-1-AP (ACSL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 13989-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 13989-1-AP (ACSL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 13989-1-AP

  IP result of anti-ACSL1 (IP:13989-1-AP, 4ug; Detection:13989-1-AP 1:1000) with mouse liver tissue lysate 3040 ug.

• 

  IHC staining of human lung squamous cell carcinoma using 13989-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 13989-1-AP (ACSL1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 13989-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13989-1-AP (ACSL1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13989-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13989-1-AP (ACSL1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 13989-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13989-1-AP (ACSL1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 13989-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 13989-1-AP (ACSL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: L02 cells, mouse cerebellum tissue, mouse liver tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human lung squamous cell carcinoma tissue, human heart tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 47 publications below
• IHC: See 7 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Product Information

13989-1-AP targets ACSL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACSL1 fusion protein Ag5059
• Full Name: acyl-CoA synthetase long-chain family member 1
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 68 kDa, 78 kDa
• GenBank Accession Number: BC050073
• Gene Symbol: ACSL1
• Gene ID (NCBI): 2180
• RRID: AB_2257870
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACSL1(Long-chain-fatty-acid--CoA ligase 1) is also named as FACL1, FACL2, LACS, LACS1, LACS2 and belongs to the ATP-dependent AMP-binding enzyme family. ACSL1 is a 75 kDa protein that is associated peripherally with the plasma membrane(Brian M Wiczer, etc., 2006). ACSL1 is abundantly expressed in tissues, such as liver and brown fat, that metabolize substantial amounts of triglycerides as fuel, and as such, a deficiency in ACSL1 function could have a more profound affect in those cells, resulting in hepatosteatosis and potentially increased very low density lipoprotein production by the liver or decreased thermogenic capacity in brown adipose tissue(PMID:19429676). An anti-rat ACSL1 antibody recognized a band of the predicted 68 kDa in high-speed supernatant from rat liver and in human and murine SMCs, monocyte-derived macrophages, and murine peritoneal macrophages (PMID:17259370). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Nature; 7-Dehydrocholesterol dictates ferroptosis sensitivity; Authors - Yaxu Li
mouse	IF	Nat Cell Biol; Functional multi-organelle units control inflammatory lipid metabolism of macrophages; Authors - Julia A Zimmermann
human	WB	Hepatology; Acetyl-CoA carboxylase α promotion of glucose-mediated fatty acid synthesis enhances survival of hepatocellular carcinoma in mice and patients.; Authors - Ming-Da Wang
human	IF	Theranostics; Mesenchymal glioblastoma constitutes a major ceRNA signature in the TGF-β pathway.; Authors - Qixue Wang
human	WB, IHC	Cancer Res; Long noncoding RNA HULC modulates abnormal lipid metabolism in hepatoma cells through an miR-9-mediated RXRA signaling pathway.; Authors - Ming Cui
mouse	WB	Oxid Med Cell Longev; Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.; Authors - Wei Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Thomas (Verified Customer) (10-23-2019)

Very good service, very good antibody

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: Rattus heart