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ACSS1 Polyclonal antibody
ACSS1 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (1)
WB, IP, IHC, IF, ELISA
Cat no : 17138-1-AP
Validation Data Gallery
|Positive WB detected in||Caco-2 cells, Jurkat cells, mouse kidney tissue, RAW 264.7 cells|
|Positive IP detected in||mouse kidney tissue|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 2 publications below|
|WB||See 8 publications below|
|IHC||See 2 publications below|
|IF||See 1 publications below|
17138-1-AP targets ACSS1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ACSS1 fusion protein Ag10896|
|Full Name||acyl-CoA synthetase short-chain family member 1|
|Calculated molecular weight||689 aa, 75 kDa|
|Observed molecular weight||70-75 kDa|
|GenBank accession number||BC039261|
|Gene ID (NCBI)||84532|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
|Product Specific Protocols|
|WB protocol for ACSS1 antibody 17138-1-AP||Download protocol|
|IHC protocol for ACSS1 antibody 17138-1-AP||Download protocol|
|IP protocol for ACSS1 antibody 17138-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Sex-specific, reciprocal regulation of ERα and miR-22 controls muscle lipid metabolism in male mice.
J Cell Physiol
Acetyl-CoA synthases are essential for maintaining histone acetylation under metabolic stress during zygotic genome activation in pigs.
PLoS Comput Biol
Inferring latent temporal progression and regulatory networks from cross-sectional transcriptomic data of cancer samples.
Integrated Metabolomics and Network Pharmacology Approach to Explain Possible Action Mechanisms of Xin-Sheng-Hua Granule for Treating Anemia.
J Biol Chem
Glucose-independent acetate metabolism promotes melanoma cell survival and tumor growth.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Marina (Verified Customer) (12-18-2020)
I got different bands and none at 70kDa. I'm not sure if it is related to ACSS1 antibody quality, or if my cells don't express this protein...
Tanusree (Verified Customer) (12-18-2019)
Product worked well in WB at 1:500 dilution