• Featured Product
  • KD/KO Validated

ACSS1 Polyclonal antibody

ACSS1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17138-1-AP

Synonyms

ACAS2L, AceCS2, AceCS2L, Acetate CoA ligase 2, Acetyl CoA synthetase 2, ACSS1, KIAA1846



Tested Applications

Positive WB detected inCaco-2 cells, Jurkat cells, mouse kidney tissue, RAW 264.7 cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

17138-1-AP targets ACSS1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACSS1 fusion protein Ag10896
Full Name acyl-CoA synthetase short-chain family member 1
Calculated molecular weight 689 aa, 75 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC039261
Gene symbol ACSS1
Gene ID (NCBI) 84532
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACSS1 antibody 17138-1-APDownload protocol
IHC protocol for ACSS1 antibody 17138-1-APDownload protocol
IP protocol for ACSS1 antibody 17138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Biochem J

NRF2/ACSS2 axis mediates the metabolic effect of alcohol drinking on esophageal squamous cell carcinoma.

Authors - Joab Otieno Odera
humanWB

J Biol Chem

Glucose-independent acetate metabolism promotes melanoma cell survival and tumor growth.

Authors - Alexander J Lakhter
  • KD Validated
ratWB

Front Pharmacol

Integrated Metabolomics and Network Pharmacology Approach to Explain Possible Action Mechanisms of Xin-Sheng-Hua Granule for Treating Anemia.

Authors - Han-Qing Pang
humanWB, IHC

PLoS Comput Biol

Inferring latent temporal progression and regulatory networks from cross-sectional transcriptomic data of cancer samples.

Authors - Xiaoqiang Sun
pigIF,WB

J Cell Physiol

Acetyl-CoA synthases are essential for maintaining histone acetylation under metabolic stress during zygotic genome activation in pigs.

Authors - Wenjun Zhou
  • KO Validated
humanWB

EMBO J

Sex-specific, reciprocal regulation of ERα and miR-22 controls muscle lipid metabolism in male mice.

Authors - Judith Schweisgut

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Marina (Verified Customer) (12-18-2020)

I got different bands and none at 70kDa. I'm not sure if it is related to ACSS1 antibody quality, or if my cells don't express this protein...

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Macrophages
ACSS1 Antibody Western Blot validation (1:500 dilution) in Macrophages (Cat no:17138-1-AP)
FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain