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ACSS1 Polyclonal antibody
ACSS1 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 17138-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Caco-2 cells, Jurkat cells, mouse kidney tissue, RAW 264.7 cells |
Positive IP detected in | mouse kidney tissue |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 8 publications below |
IHC | See 2 publications below |
IF | See 1 publications below |
Product Information
17138-1-AP targets ACSS1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ACSS1 fusion protein Ag10896 |
Full Name | acyl-CoA synthetase short-chain family member 1 |
Calculated molecular weight | 689 aa, 75 kDa |
Observed molecular weight | 70-75 kDa |
GenBank accession number | BC039261 |
Gene symbol | ACSS1 |
Gene ID (NCBI) | 84532 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for ACSS1 antibody 17138-1-AP | Download protocol |
IHC protocol for ACSS1 antibody 17138-1-AP | Download protocol |
IP protocol for ACSS1 antibody 17138-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
EMBO J Sex-specific, reciprocal regulation of ERα and miR-22 controls muscle lipid metabolism in male mice. | ||
J Cell Physiol Acetyl-CoA synthases are essential for maintaining histone acetylation under metabolic stress during zygotic genome activation in pigs.
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PLoS Comput Biol Inferring latent temporal progression and regulatory networks from cross-sectional transcriptomic data of cancer samples. | ||
Front Pharmacol Integrated Metabolomics and Network Pharmacology Approach to Explain Possible Action Mechanisms of Xin-Sheng-Hua Granule for Treating Anemia. | ||
J Biol Chem Glucose-independent acetate metabolism promotes melanoma cell survival and tumor growth.
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Marina (Verified Customer) (12-18-2020) | I got different bands and none at 70kDa. I'm not sure if it is related to ACSS1 antibody quality, or if my cells don't express this protein...
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FH Tanusree (Verified Customer) (12-18-2019) | Product worked well in WB at 1:500 dilution
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