Tested Applications
| Positive WB detected in | Caco-2 cells, Jurkat cells, mouse kidney tissue, RAW 264.7 cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17138-1-AP targets ACSS1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10896 Product name: Recombinant human ACSS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 340-689 aa of BC039261 Sequence: ITGHSYVVYGPLCNGATSVLFESTPVYPNAGRYWETVERLKINQFYGAPTAVRLLLKYGDAWVKKYDRSSLRTLGSVGEPINCEAWEWLHRVVGDSRCTLVDTWWQTETGGICIAPRPSEEGAEILPAMAMRPFFGIVPVLMDEKGSVMEGSNVSGALCISQAWPGMARTIYGDHQRFVDAYFKAYPGYYFTGDGAYRTEGGYYQITGRMDDVINISGHRLGTAEIEDAIADHPAVPESAVIGYPHDIKGEAAFAFIVVKDSAGDSDVVVQELKSMVATKIAKYAVPDEILVVKRLPKTRSGKVMRRLLRKIITSEAQELGDTTTLEDPSIIAEILSVYQKCKDKQAAAK Predict reactive species |
| Full Name | acyl-CoA synthetase short-chain family member 1 |
| Calculated Molecular Weight | 689 aa, 75 kDa |
| Observed Molecular Weight | 70-75 kDa |
| GenBank Accession Number | BC039261 |
| Gene Symbol | ACSS1 |
| Gene ID (NCBI) | 84532 |
| RRID | AB_2289182 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NUB1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The ACSS (acetyl-CoA synthetase) enzyme is the sole known mammalian enzyme that can catalyze the conversion of free acetate into acetyl coenzyme A (acetyl-CoA). The three known isoforms of human ACSS are termed ACSS1, ACSS2, and ACSS3. The main substrate of ACSS1 and ACSS2 is acetate, while the preferential substrate of ACSS3 is propionate. Two acetate related enzymes, ACSS1(GeneID: 84532) and ACSS2 (GeneID:55902) difer in their tissue distribution and subcellular localization. On the one hand, as a mitochondrial matrix enzyme, ACSS1 is expressed mainly in cardiac and skeletal muscle as well as brown adipose tissue. On the other hand, as a nuclear and cytoplasmic enzyme, ACSS2 is strongly expressed in the liver, kidney and heart and moderately expressed in the brain and testis.ACSS2 participates in lipid synthesis and facilitates protein acetylation by generating acetyl-CoA, while ACSS1 is involved in acetate oxidation. The functional diferences in these enzymes involve energy production through the tricarboxylic acid (TCA) cycle. Due to its more thorough utilization of intracellular acetate, ACSS2 is expressed in almost all cell types under diferent physiological conditions.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for ACSS1 antibody 17138-1-AP | Download protocol |
| WB protocol for ACSS1 antibody 17138-1-AP | Download protocol |
| IHC protocol for ACSS1 antibody 17138-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ACSS1 Polyclonal antibody (Cat# 17138-1-AP) has 32 citations appearing in journals such as Nature, Cell Metabolism, Nature Cancer, Nature Metabolism, Cancer Research, Nature Communications, Cell Reports, EMBO Journal, Cell Death & Disease, and BMC Genomics. Associated research fields include acetate and acetyl-CoA metabolism, epigenetic regulation, cancer biology (breast cancer, glioblastoma, melanoma, lymphoma, colorectal cancer), ferroptosis, lipid metabolism, immune regulation, liver disease, and metabolic reprogramming across human and mouse models.
| Species | Application | Title |
|---|---|---|
Cell Metab Metabolic regulation of the glioblastoma stem cell epitranscriptome by malate dehydrogenase 2 | ||
Cell Metab Acetate enables metabolic fitness and cognitive performance during sleep disruption | ||
Nat Cancer Acetate acts as a metabolic immunomodulator by bolstering T-cell effector function and potentiating antitumor immunity in breast cancer | ||
Nat Metab Acetate reprogrammes tumour metabolism and promotes PD-L1 expression and immune evasion by upregulating c-Myc | ||
Cancer Res Mitochondrial Calcium Uniporter Links Acetyl-CoA Metabolism and H3K27 Acetylation to Maintain Glioblastoma Stem Cells
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Reviews
What customers say
Two reviewers rated this antibody for Western Blot. One reported satisfactory performance at a 1:500 dilution in mouse brain tissue. The other, also using 1:500 in macrophages, observed unexpected banding patterns with no signal at the expected 70 kDa, expressing uncertainty about whether the issue stemmed from the antibody or low target expression in their cell line. Overall, feedback is mixed, with one positive and one concerning experience.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Marina (Verified Customer) (12-18-2020) | I got different bands and none at 70kDa. I'm not sure if it is related to ACSS1 antibody quality, or if my cells don't express this protein...
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Tanusree (Verified Customer) (12-18-2019) | Product worked well in WB at 1:500 dilution
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