ACSS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ACSS1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (2)

Cat no : 17138-1-AP

Synonyms

ACAS2L, AceCS2, AceCS2L, Acetate CoA ligase 2, Acetyl CoA synthetase 2, ACSS1, KIAA1846

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Validation Data Gallery

• 

  WB analysis using 17138-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17138-1-AP (ACSS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 17138-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 17138-1-AP (ACSS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 17138-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 17138-1-AP (ACSS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17138-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17138-1-AP (ACSS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17138-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17138-1-AP (ACSS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17138-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17138-1-AP (ACSS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 17138-1-AP

  IP Result of anti-ACSS1 (IP:17138-1-AP, 4ug; Detection:17138-1-AP 1:700) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human liver cancer using 17138-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17138-1-AP (ACSS1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 17138-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17138-1-AP (ACSS1 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: Caco-2 cells, Jurkat cells, mouse kidney tissue, RAW 264.7 cells
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:100-1:400

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

17138-1-AP targets ACSS1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACSS1 fusion protein Ag10896
• Full Name: acyl-CoA synthetase short-chain family member 1
• Calculated molecular weight: 689 aa, 75 kDa
• Observed molecular weight: 70-75 kDa
• GenBank accession number: BC039261
• Gene symbol: ACSS1
• Gene ID (NCBI): 84532
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Nat Commun; PMID: 27357947; Authors - Xue Gao
human	WB	EMBO J; Sex-specific, reciprocal regulation of ERα and miR-22 controls muscle lipid metabolism in male mice.; Authors - Judith Schweisgut
pig	IF,WB	J Cell Physiol; Acetyl-CoA synthases are essential for maintaining histone acetylation under metabolic stress during zygotic genome activation in pigs.; Authors - Wenjun Zhou ; KO Validated
human	WB, IHC	PLoS Comput Biol; Inferring latent temporal progression and regulatory networks from cross-sectional transcriptomic data of cancer samples.; Authors - Xiaoqiang Sun
rat	WB	Front Pharmacol; Integrated Metabolomics and Network Pharmacology Approach to Explain Possible Action Mechanisms of Xin-Sheng-Hua Granule for Treating Anemia.; Authors - Han-Qing Pang
human	WB	J Biol Chem; Glucose-independent acetate metabolism promotes melanoma cell survival and tumor growth.; Authors - Alexander J Lakhter ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Marina (Verified Customer) (12-18-2020)

I got different bands and none at 70kDa. I'm not sure if it is related to ACSS1 antibody quality, or if my cells don't express this protein...

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Macrophages

ACSS1 Antibody Western Blot validation (1:500 dilution) in Macrophages (Cat no:17138-1-AP)

FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain