ACSS1 Polyclonal antibody

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  WB analysis using 17138-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17138-1-AP (ACSS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17138-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17138-1-AP (ACSS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 17138-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17138-1-AP (ACSS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 17138-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 17138-1-AP (ACSS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17138-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17138-1-AP (ACSS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 17138-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 17138-1-AP (ACSS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 17138-1-AP

  IP result of anti-ACSS1 (IP:17138-1-AP, 4ug; Detection:17138-1-AP 1:700) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human liver cancer using 17138-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17138-1-AP (ACSS1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 17138-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17138-1-AP (ACSS1 Antibody) at dilution of 1:200 (under 40x lens).

• Featured Product
• KD/KO Validated

ACSS1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 17138-1-AP

Publications(18)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

ACAS2L, ACECS1, AceCS2L, Acetyl coenzyme A synthetase 2 like, mitochondrial, ACSS1, KIAA1846

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Tested Applications

• Positive WB detected in: Caco-2 cells, Jurkat cells, mouse kidney tissue, RAW 264.7 cells
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

17138-1-AP targets ACSS1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACSS1 fusion protein Ag10896
• Full Name: acyl-CoA synthetase short-chain family member 1
• Calculated Molecular Weight: 689 aa, 75 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC039261
• Gene Symbol: ACSS1
• Gene ID (NCBI): 84532
• RRID: AB_2289182
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NUB1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ACSS (acetyl-CoA synthetase) enzyme is the sole known mammalian enzyme that can catalyze the conversion of free acetate into acetyl coenzyme A (acetyl-CoA). The three known isoforms of human ACSS are termed ACSS1, ACSS2, and ACSS3. The main substrate of ACSS1 and ACSS2 is acetate, while the preferential substrate of ACSS3 is propionate. Two acetate related enzymes, ACSS1(GeneID: 84532) and ACSS2 (GeneID:55902) difer in their tissue distribution and subcellular localization. On the one hand, as a mitochondrial matrix enzyme, ACSS1 is expressed mainly in cardiac and skeletal muscle as well as brown adipose tissue. On the other hand, as a nuclear and cytoplasmic enzyme, ACSS2 is strongly expressed in the liver, kidney and heart and moderately expressed in the brain and testis.ACSS2 participates in lipid synthesis and facilitates protein acetylation by generating acetyl-CoA, while ACSS1 is involved in acetate oxidation. The functional diferences in these enzymes involve energy production through the tricarboxylic acid (TCA) cycle. Due to its more thorough utilization of intracellular acetate, ACSS2 is expressed in almost all cell types under diferent physiological conditions.

Publications

Species	Application	Title
mouse	WB,IF	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
human	WB,IHC	Nat Commun; PMID: 27357947; Authors - Xue Gao
human	WB	EMBO J; Sex-specific, reciprocal regulation of ERα and miR-22 controls muscle lipid metabolism in male mice.; Authors - Judith Schweisgut
pig	IF,WB	J Cell Physiol; Acetyl-CoA synthases are essential for maintaining histone acetylation under metabolic stress during zygotic genome activation in pigs.; Authors - Wenjun Zhou ; KO Validated
human	WB, IHC	PLoS Comput Biol; Inferring latent temporal progression and regulatory networks from cross-sectional transcriptomic data of cancer samples.; Authors - Xiaoqiang Sun
rat	WB	Front Pharmacol; Integrated Metabolomics and Network Pharmacology Approach to Explain Possible Action Mechanisms of Xin-Sheng-Hua Granule for Treating Anemia.; Authors - Han-Qing Pang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marina (Verified Customer) (12-18-2020)

I got different bands and none at 70kDa. I'm not sure if it is related to ACSS1 antibody quality, or if my cells don't express this protein...

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Macrophages

ACSS1 Antibody Western Blot validation (1:500 dilution) in Macrophages (Cat no:17138-1-AP)

FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain