|Positive WB detected in||Caco-2 cells, Jurkat cells, mouse kidney tissue, RAW 264.7 cells|
|Positive IP detected in||mouse kidney tissue|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Sample-dependent, check data in validation data gallery|
17138-1-AP targets ACSS1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, pig, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ACSS1 fusion protein Ag10896|
|Full Name||acyl-CoA synthetase short-chain family member 1|
|Calculated molecular weight||689 aa, 75 kDa|
|Observed molecular weight||70-75 kDa|
|GenBank accession number||BC039261|
|Gene ID (NCBI)||84532|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Marina (Verified Customer) (12-18-2020)
I got different bands and none at 70kDa. I'm not sure if it is related to ACSS1 antibody quality, or if my cells don't express this protein...
Tanusree (Verified Customer) (12-18-2019)
Product worked well in WB at 1:500 dilution